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Publication Type J
Authors Smith, TM; York, PH; Stanley, AM; Macreadie, PI; Keough, MJ; Ross, DJ; Sherman, CDH
Author Full Name Smith, Timothy M.; York, Paul H.; Stanley, Annalise M.; Macreadie, Peter I.; Keough, Michael J.; Ross, D. Jeff; Sherman, Craig D. H.
Title Microsatellite primer development for the seagrass Zostera nigricaulis (Zosteraceae)
Source CONSERVATION GENETICS RESOURCES
Language English
Document Type Article
Author Keywords Zostera nigricaulis; Microsatellite markers; Cross-amplification; Genetic structure; Seagrass
Keywords Plus AUSTRALIA; MARKERS
Abstract Seagrasses are marine angiosperms with a worldwide distribution that form conspicuous beds in nearshore habitats. Despite being universally recognised as a foundation species that performs a number of important ecosystems functions (incl. sediment stabilisation, facilitation of biodiversity, nutrient cycling and carbon sequestration), global seagrass habitats are in decline. Resilience-the ability to recover from disturbance without switching to an alternative state-is paramount to the maintenance and persistence of seagrass habitats. Genetic diversity is a key component of seagrass resilience and contributes to an understanding of population structure, connectivity between populations, and reproductive strategies. Microsatellite primers were developed to investigate the resilience of the seagrass Zostera nigricaulis, which dominates subtidal habitats in the bays of south-eastern Australia. We also tested for cross-amplification of markers between Z. nigricaulis and previously developed markers for the sympatric species Z. muelleri to assess their applicability for use in assessing patterns of genetic diversity, population structure, and mating system. Using next-generation sequencing we isolated 11 novel microsatellite loci for Z. nigricaulis, 8 of which were polymorphic for the samples tested. Allelic diversity ranged from 1 to 8. None of the primer pairs developed for Z. nigricaulis cross-amplified in Z. muelleri; but 14 of 24 primer pairs previously developed for Z. muelleri amplified clearly in Z. nigricaulis samples with six of these showing polymorphism. The results demonstrate the applicability of the Z. nigricaulis microsatellite primers for use in the study of population genetics and limited cross-amplification with Z. muelleri.
Author Address [Smith, Timothy M.; York, Paul H.; Stanley, Annalise M.; Sherman, Craig D. H.] Deakin Univ, Sch Life & Environm Sci, Ctr Integrat Ecol, Geelong, Vic 3217, Australia; [Macreadie, Peter I.] Univ Technol Sydney, Sch Environm, Plant Funct Biol & Climate Change Cluster C3, Sydney, NSW 2007, Australia; [Keough, Michael J.] Univ Melbourne, Dept Zool, Melbourne, Vic 3010, Australia; [Ross, D. Jeff] Univ Tasmania, Inst Marine & Antarctic Studies, Hobart, Tas 7053, Australia
Reprint Address Smith, TM (reprint author), Deakin Univ, Sch Life & Environm Sci, Ctr Integrat Ecol, Pigdons Rd, Geelong, Vic 3217, Australia.
E-mail Address tim.smith@deakin.edu.au
ResearcherID Number York, Paul/C-4120-2009; Sherman, Craig/A-2484-2010; Smith, Timothy/C-9283-2013; Ross, Donald/F-7607-2012
ORCID Number York, Paul/0000-0003-3530-4146; Sherman, Craig/0000-0003-2099-0462; Smith, Timothy/0000-0001-8612-8600; Ross, Donald/0000-0002-8659-3833
Funding Agency and Grant Number Victorian Department of Sustainability and Environment
Funding Text The authors would like to thank A. Hirst, N. Hutchinson, C. White and D. Hatton for assistance with specimen collection. This study was financially supported by a research grant from the Victorian Department of Sustainability and Environment: 'Seagrass resilience in Port Phillip Bay: developing better predictions of how seagrasses respond to environmental change'.
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Cited Reference Count 12
Times Cited 6
Total Times Cited Count (WoS, BCI, and CSCD) 6
Publisher SPRINGER
Publisher City DORDRECHT
Publisher Address VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
ISSN 1877-7252
29-Character Source Abbreviation CONSERV GENET RESOUR
ISO Source Abbreviation Conserv. Genet. Resour.
Publication Date SEP
Year Published 2013
Volume 5
Issue 3
Beginning Page 607
Ending Page 610
Digital Object Identifier (DOI) 10.1007/s12686-013-9862-3
Page Count 4
Web of Science Category Biodiversity Conservation; Genetics & Heredity
Subject Category Biodiversity & Conservation; Genetics & Heredity
Document Delivery Number 194GT
Unique Article Identifier WOS:000322619900003
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