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Publication Type J
Authors Ghneim-Herrera, T., M. G. Selvaraj, D. Meynard, D. Fabre, A. Pena, W. Ben Romdhane, R. Ben Saad, S. Ogawa, M. C. Rebolledo, M. Ishitani, J. Tohme, A. Al-Doss, E. Guiderdoni and A. Hassairi
Title Expression of the Aeluropus littoralis AlSAP Gene Enhances Rice Yield under Field Drought at the Reproductive Stage
Source Frontiers in Plant Science
Author Keywords AlSAP A20/AN1 stress-associated protein drought reproductive stage rice yield oryza-sativa l. a20/an1 zinc-finger abiotic stress-response water-deficit stress transgenic rice grain-yield transcription factor confers drought tolerance resistance
Abstract We evaluated the yields of Oryza sativa L. 'Nipponbare' rice lines expressing a gene encoding an A20/AN1 domain stress-associated protein, AlSAP, from the halophyte grass Aeluropus littoralis under the control of different promoters. Three independent field trials were conducted, with drought imposed at the reproductive stage. In all trials, the two transgenic lines, RN5 and RN6, consistently out-performed non-transgenic (NT) and wild-type (WT) controls, providing 50-90% increases in grain yield (GY). Enhancement of tillering and panicle fertility contributed to this improved GY under drought. In contrast with physiological records collected during previous greenhouse dry-down experiments, where drought was imposed at the early tillering stage, we did not observe significant differences in photosynthetic parameters, leaf water potential, or accumulation of antioxidants in flag leaves of AlSAP-lines subjected to drought at flowering. However, AlSAP expression alleviated leaf rolling and leaf drying induced by drought, resulting in increased accumulation of green biomass. Therefore, the observed enhanced performance of the AlSAP-lines subjected to drought at the reproductive stage can be tentatively ascribed to a primed status of the transgenic plants, resulting from a higher accumulation of biomass during vegetative growth, allowing reserve remobilization and maintenance of productive tillering and grain filling. Under irrigated conditions, the overall performance of AlSAP-lines was comparable with, or even significantly better than, the NT and WT controls. Thus, AlSAP expression inflicted no penalty on rice yields under optimal growth conditions. Our results support the use of AlSAP transgenics to reduce rice GY losses under drought conditions.
Author Address [Ghneim-Herrera, Thaura; Pena, Alexandra] Univ Icesi, Dept Ciencias Biol, Cali, Colombia. [Selvaraj, Michael G.; Ogawa, Satoshi; Rebolledo, Maria C.; Ishitani, Manabu; Tohme, Joe] Int Ctr Trop Agr, Cali, Colombia. [Meynard, Donaldo; Fabre, Denis; Guiderdoni, Emmanuel] Ctr Cooperat Int Rech Agron Dev, UMR Ameliorat Genet & Adaptat Plantes Mediterrane, Montpellier, France. [Ben Romdhane, Walid; Al-Doss, Abdullah] King Saud Univ, Dept Plant Prod, Coll Food & Agr Sci, Riyadh, Saudi Arabia. [Ben Saad, Rania] Univ Sfax, Ctr Biotechnol Sfax, Biotechnol & Plant Improvement Lab, Sfax, Tunisia. [Ogawa, Satoshi] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Global Agr Sci, Tokyo, Japan. [Hassairi, Afif] Ctr Biotechnol Sfax, Sfax, Tunisia. Ghneim-Herrera, T (reprint author), Univ Icesi, Dept Ciencias Biol, Cali, Colombia.; Selvaraj, MG (reprint author), Int Ctr Trop Agr, Cali, Colombia.; Guiderdoni, E (reprint author), Ctr Cooperat Int Rech Agron Dev, UMR Ameliorat Genet & Adaptat Plantes Mediterrane, Montpellier, France. tghneim@icesi.edu.co; m.selvaraj@cgiar.org; guiderdoni@cirad.fr
ISSN 1664-462X
ISBN 1664-462X
29-Character Source Abbreviation Front. Plant Sci.
Publication Date Jun
Year Published 2017
Volume 8
Digital Object Identifier (DOI) 10.3389/fpls.2017.00994
Unique Article Identifier WOS:000403043800001

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