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Publication Type J
Authors Abdallah, H. M. and A. Esmat
Title Antioxidant and anti-inflammatory activities of the major phenolics from Zygophyllum simplex L
Source Journal of Ethnopharmacology
Author Keywords Anti-inflammatory Anti-oxidant Flavonoids Zygophyllum simplex gene-expression constituents flavonoids luteolin
Abstract Ethnopharmacological relevance: Zygophyllum simplex L. is a halophyte plant that follows Zygophyllaceae. The plant is growing in arid and semiarid regions. It has been used traditionally in Arabic region to treat gout, asthma and inflammation. Aim of study: Although ant-inflammatory activity has been reported for this plant, this study aimed to isolate and identify the major constituents of Zygophyllum simplex L., as well as assessing their antioxidant and anti-inflammatory activities in-vitro. In this study, the mechanism of anti-inflammatory activity of the isolated compounds was assessed. Materials and methods: Defatted fraction of the total methanol extract of the aerial parts of Z. simplex was repeatedly chromatographed on Diaion HP-20, polyamide, and RP18 columns to give five major phenolic compounds. The identity of the purified compounds was established by NMR experiments and comparing with previously known analogs. Moreover, the antioxidant and anti-inflammatory activities of the purified phenolics were investigated in-vitro through measuring of NF kappa B, PGE(2), IL-6, IL-1 beta and TNF-alpha levels in human peripheral blood mononuclear cells (PBMC) stimulated with phytohaemagglutinin (PHA). Results: Phytochemical investigation of the flowering aerial parts of Z. simplex resulted in isolation of five major metabolites identified as isorhamnetin-3-O-beta-D-rutinoside (1), myricitrin (2), luteolin-7-O-beta-D-glucoside (3), isorhamnetin-3-O-beta-D-glucoside(4), and isorhamnetin (5). It is noteworthy to report that compounds 1-3 were isolated from the plant for the first time. It was reported that NF kappa B represents an important linkage between oxidative stress and inflammation. Compounds 2 and 3 have exhibited the highest antioxidant activity and showed the most efficient in decreasing NF kappa B p65 at the lowest concentration (1 mu M). Moreover; at 1 mu M concentrations, only compounds 2 and 3 significantly decreased IL-6, IL-1 beta and TNF-alpha levels from PHA treatment. Nevertheless, at 100 all isolated metabolites significantly decreased IL-6 compared to PHA treatment. Conclusion: Five major phenolic compounds were isolated from Z. simplex. Anti-inflammatory activity exhibited by the isolated compounds augment the traditional use of this plant as anti-inflammatory. The effect was mediated via inhibition of NF kappa B through antioxidant mechanism and subsequent inhibition to other inflammatory mediators like TNF-alpha, IL-1 beta and IL-6.
Author Address [Abdallah, Hossam M.] King Abdulaziz Univ, Fac Pharm, Dept Nat Prod & Alternat Med, Jeddah 21589, Saudi Arabia. [Abdallah, Hossam M.] Cairo Univ, Fac Pharm, Dept Pharmacognosy, Cairo 11562, Egypt. [Esmat, Ahmed] Ain Shams Univ, Fac Pharm, Dept Pharmacol & Toxicol, Cairo 11566, Egypt. Abdallah, HM (reprint author), King Abdulaziz Univ, Fac Pharm, Dept Nat Prod & Alternat Med, Jeddah 21589, Saudi Arabia. hmafifi2013@gmail.com
ISSN 0378-8741
ISBN 0378-8741
29-Character Source Abbreviation J. Ethnopharmacol.
Publication Date Jun
Year Published 2017
Volume 205
Beginning Page 51-56
Digital Object Identifier (DOI) 10.1016/j.jep.2017.04.022
Unique Article Identifier WOS:000404703900007

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