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Publication Type J
Authors Duro, N; Batista-Santos, P; da Costa, M; Maia, R; Castro, IV; Ramos, M; Ramalho, JC; Pawlowski, K; Maguas, C; Ribeiro-Barros, A
Author Full Name Duro, Nuno; Batista-Santos, Paula; da Costa, Mario; Maia, Rodrigo; Castro, Isabel V.; Ramos, Margarida; Ramalho, Jose C.; Pawlowski, Katharina; Maguas, Cristina; Ribeiro-Barros, Ana
Title The impact of salinity on the symbiosis between Casuarina glauca Sieb. ex Spreng. and N-2-fixing Frankia bacteria based on the analysis of Nitrogen and Carbon metabolism
Source PLANT AND SOIL
Language English
Document Type Article
Author Keywords Casuarina glauca; Frankia; Actinorhizal nodules; Salt stress
Keywords Plus ROOT-NODULE SYMBIOSIS; SALT STRESS; ACTINORHIZAL SYMBIOSES; ISOTOPE DISCRIMINATION; ASPARAGINE SYNTHETASE; OSMOTIC ADJUSTMENT; NATURAL-ABUNDANCE; HIGHER-PLANTS; WHOLE-PLANT; TOLERANCE
Abstract Casuarina glauca is an actinorhizal plant that establishes root-nodule symbiosis with N-2-fixing bacteria of the genus Frankia. This plant is highly recalcitrant to extreme environmental conditions such as salinity and drought. The aim of this study was to evaluate the impact of salt stress on the symbiotic relationship between C. glauca and Frankia Thr, focusing on N and C metabolism. Symbiotic and non-symbiotic plants were exposed to 0, 200, 400 and 600 mM NaCl. The following analyses were performed: stable carbon (delta C-13) and nitrogen (delta N-15) isotope signature; nitrogenase activity in nodules (acetylene reduction assay); and gene expression of a set of genes involved in nodule infection and N/C metabolism (qRT-PCR). Data were analysed using two-way ANOVA. Salt stress induced an enrichment in delta C-13 and delta N-15, reflecting a negative impact of salt in the relative water content and N-2 fixation, respectively. Furthermore, nitrogenase activity in nodules was insignificant already at 200 mM NaCl, consistent with the expression patterns of nifH as well as of plant genes involved in nodule induction and metabolism. The ability of C. glauca to thrive under highly saline environments is not dependent on the symbiosis with Frankia.
Author Address [Duro, Nuno; Batista-Santos, Paula; da Costa, Mario; Ramalho, Jose C.; Ribeiro-Barros, Ana] Univ Lisbon, ISA, Plant Stress & Biodivers Grp, Av Republ, P-2784505 Oeiras, Portugal; [Duro, Nuno; da Costa, Mario; Ribeiro-Barros, Ana] Univ Nova Lisboa, Inst Tecnol Quim & Biol, Av Republ,Quinta Marques, P-2780157 Oeiras, Portugal; [Maia, Rodrigo; Ramos, Margarida; Maguas, Cristina] Univ Lisbon, Fac Ciencias, Ctr Ecol Evolut & Environm Changes CE3c, P-1749016 Lisbon, Portugal; [Castro, Isabel V.] Inst Nacl Invest Agr & Vet, IP, Unidade Estrateg Invest Sistemas Agr & Florestais, Av Republ, P-2784159 Oeiras, Portugal; [Ramalho, Jose C.; Ribeiro-Barros, Ana] Univ Nova Lisboa, Fac Ciencias Tecnol, BioGeoTec, P-2829516 Caparica, Portugal; [Pawlowski, Katharina] Stockholm Univ, Dept Ecol Environm & Plant Sci, S-10691 Stockholm, Sweden
Reprint Address Ribeiro-Barros, A (reprint author), Univ Lisbon, ISA, Plant Stress & Biodivers Grp, Av Republ, P-2784505 Oeiras, Portugal.; Ribeiro-Barros, A (reprint author), Univ Nova Lisboa, Inst Tecnol Quim & Biol, Av Republ,Quinta Marques, P-2780157 Oeiras, Portugal.
E-mail Address aribeiro@itqb.unl.pt
ORCID Number RIBEIRO, ANA/0000-0002-6071-6460
Funding Agency and Grant Number Fundacao para a Ciencia e Tecnologia [PTDC/AGR-FOR/4218/2012, SFRH/BPD/78619/2011]
Funding Text This work was supported by Fundacao para a Ciencia e Tecnologia under the scope of the project PTDC/AGR-FOR/4218/2012 and grant SFRH/BPD/78619/2011 (P. Batista-Santos). The authors acknowledge Paula Alves for technical assistance.
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Cited Reference Count 59
Times Cited 4
Total Times Cited Count (WoS, BCI, and CSCD) 4
Publisher SPRINGER
Publisher City DORDRECHT
Publisher Address VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
ISSN 0032-079X
29-Character Source Abbreviation PLANT SOIL
ISO Source Abbreviation Plant Soil
Publication Date JAN
Year Published 2016
Volume 398
Issue 1-2
Beginning Page 327
Ending Page 337
Digital Object Identifier (DOI) 10.1007/s11104-015-2666-3
Page Count 11
Web of Science Category Agronomy; Plant Sciences; Soil Science
Subject Category Agriculture; Plant Sciences
Document Delivery Number DB5JP
Unique Article Identifier WOS:000368550700025
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