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Version 3.20
Publication Type J
Authors Jorge, TF; Florencio, MH; Ribeiro-Barros, AI; Antonio, C
Author Full Name Jorge, Tiago F.; Helena Florencio, M.; Ribeiro-Barros, Ana I.; Antonio, Carla
Title Quantification and structural characterization of raffinose family oligosaccharides in Casuarina glauca plant tissues by porous graphitic carbon electrospray quadrupole ion trap mass spectrometry
Language English
Document Type Article
Author Keywords Raffinose family oligosaccharides; Tandem mass spectrometry; Carbohydrate fragmentation; Porous graphitic carbon; Casuariana glauca; Salinity stress
Abstract Raffinose family oligosaccharides (RFOs) are non-structural, water-soluble carbohydrates widely distributed in the plant kingdom, and include the trisaccharide raffinose, the tetrasaccharide stachyose and the pentasaccharide verbascose. RFOs have been suggested to play a major role in storing carbohydrates in seeds and vegetative tissues, and in conferring osmoprotection against abiotic stresses, such as high salinity and drought. However, the high polarity nature of RFOs makes their analysis with typical reversed phase liquid chromatography (RP-LC) generally difficult, with these compounds eluting very close to the void volume with minimal retention. Moreover, carbohydrate-related compounds lack an inherent chromophore in the ultraviolet-visible (UV) region to make them suitable for UV detection. To overcome these issues, alternative analytical methods based on LC coupled to mass spectrometry (MS) have been developed and porous graphitic carbon (PGC) stationary phases described in the literature as good alternative to typical RP columns for retaining these highly polar compounds were used. In addition, PGC stationary phases allow the use of MS-compatible mobile phases for efficient on-line coupling with electrospray ionization (ESI), and when coupled to tandem MS (MSn) techniques, powerful structural information can be obtained. The present work focuses on the application of a PGC-ESI-QIT-MSn method for the quantitative analysis of RFOs in Casuariana glauca plant tissues under salt stress conditions. In addition, we describe the structural characterization of RFOs through collision induced dissociation (CID) and MSn experiments using a quadrupole ion trap mass spectrometer (QIT-MS) in the ESI positive ion mode. (C) 2016 Elsevier B.V. All rights reserved.
Author Address [Jorge, Tiago F.; Ribeiro-Barros, Ana I.; Antonio, Carla] Univ Nova Lisboa ITQB NOVA, Plant Metabol Lab, Inst Tecnol Quim & Biol Antonio Xavier, Av Republ, P-2780157 Oeiras, Portugal; [Helena Florencio, M.] Univ Lisbon, Fac Ciencias, Ctr Quim & Bioquim, P-1749016 Lisbon, Portugal; [Helena Florencio, M.] Univ Lisbon, Fac Ciencias, Dept Quim & Bioquim, P-1749016 Lisbon, Portugal; [Ribeiro-Barros, Ana I.] Univ Lisbon, Sch Agr ISA, Linking Landscape Environm Agr & Food LEAF, PlantStress&Biodivers Grp,DRAT, Av Republ, P-2784505 Oeiras, Portugal; [Ribeiro-Barros, Ana I.] Univ Nova Lisboa, Fac Ciencias & Tecnol, GeoBioTec, P-2829516 Caparica, Portugal
Reprint Address Antonio, C (reprint author), Univ Nova Lisboa ITQB NOVA, Plant Metabol Lab, Inst Tecnol Quim & Biol Antonio Xavier, Av Republ, P-2780157 Oeiras, Portugal.
E-mail Address antonio@itqb.unl.pt
Funding Agency and Grant Number FCT Investigator Programme from Fundacao para a Ciencia e a Tecnologia [IF/00376/2012/CP0165/CT0003]; ITQB NOVA RD unit GreenIT [UID/Multi/04551/2013]; FCT [PD/BD/113475/2015, PTDC/AGR-FOR/4218/2012]; ITQB NOVA International PhD Programme 'Plants for Life' [PD/00035/2013]; Thermo Fisher Scientific (Runcorn, Cheshire, UK); UNICAM Sistemas Analiticos (Linda-a-Velha, Portugal); Portuguese Mass Spectrometry Network (Rede Nacional de Espectrometria de Massa, RNEM)
Funding Text This work was supported by the FCT Investigator Programme (IF/00376/2012/CP0165/CT0003) from Fundacao para a Ciencia e a Tecnologia and the ITQB NOVA R&D unit GreenIT (UID/Multi/04551/2013). T.F.J. gratefully acknowledges FCT (PD/BD/113475/2015) and the ITQB NOVA International PhD Programme 'Plants for Life' (PD/00035/2013) for the PhD fellowship. A.I.R.-B. acknowledges FCT under the scope of the project PTDC/AGR-FOR/4218/2012. C.A. gratefully acknowledges Thermo Fisher Scientific (Runcorn, Cheshire, UK), UNICAM Sistemas Analiticos (Linda-a-Velha, Portugal), and the Portuguese Mass Spectrometry Network (Rede Nacional de Espectrometria de Massa, RNEM) for support.
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Cited Reference Count 30
Publisher City AMSTERDAM
Publisher Address PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
ISSN 1387-3806
29-Character Source Abbreviation INT J MASS SPECTROM
ISO Source Abbreviation Int. J. Mass Spectrom.
Publication Date FEB
Year Published 2017
Volume 413
Special Issue SI
Beginning Page 127
Ending Page 134
Digital Object Identifier (DOI) 10.1016/j.ijms.2016.05.004
Page Count 8
Web of Science Category Physics, Atomic, Molecular & Chemical; Spectroscopy
Subject Category Physics; Spectroscopy
Document Delivery Number ES1CG
Unique Article Identifier WOS:000399264900016
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