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Publication Type J
Authors Veluthakkal, R; Dasgupta, MG
Author Full Name Veluthakkal, R.; Dasgupta, M. G.
Title Isolation and characterization of pathogen defence-related class I chitinase from the actinorhizal tree Casuarina equisetifolia
Source FOREST PATHOLOGY
Language English
Document Type Article
Keywords Plus AMINO-ACID-SEQUENCE; CIS-ACTING ELEMENTS; PLANT CHITINASES; GENE-EXPRESSION; MESSENGER-RNA; MOLECULAR-CLONING; METHYL JASMONATE; NORWAY SPRUCE; REGULATORY ELEMENTS; HETEROBASIDION-ANNOSUM
Abstract Casuarina equisetifolia has the widest distribution of all Casuarina species and is a nitrogen-fixing tree of considerable social, economic and environmental importance. Trichosporium vesiculosum, a causal agent of blister bark disease, is a serious pathogen of C. equisetifolia. In this study, a cDNA clone encoding class I chitinase (CeChi1) belonging to PR-3 family was cloned and characterized from the needle tissues of C. equisetifolia challenged with the toxic exudate of the fungal pathogen T. vesiculosum. The CeChi1 open reading frame comprised 966 nucleotides that encoded 321 amino acid residues with the molecular mass of mature protein being approximately 34 kDa. Analysis of the predicted amino acid sequence revealed the similarity of CeChi1 protein to class I chitinase from other plant species containing a hydrophobic signal peptide domain and hinge domain. The sequence also harboured a cysteine-rich chitin-binding domain and lysozyme-like domain. A hydrophobic C-terminal domain similar to the vacuole targeting sequences of class I chitinases isolated from other plants was also detected. The genomic sequence of CeChi1 indicated that the coding region contained three exons and two introns. In silico analysis of the untranslated regions revealed the presence of several cis-acting regulatory elements associated with hormonal regulation and stress responses. Quantitative real-time PCR analyses at different time points showed upregulation of the transcript during pathogen elicitation and salicylic acid signalling. However, no significant expression of CeChi1 was observed during other abiotic stress condition including wounding, water deficit, salt and heat stress revealing the specific expression of the gene during pathogenesis. This is the first report on isolation of a gene from C. equisetifolia, and the detailed functional analyses of CeChi1 will help in understanding its specific role in defence against pathogens in this tropical tree species.
Author Address [Veluthakkal, R.; Dasgupta, M. G.] Inst Forest Genet & Tree Breeding, Div Plant Biotechnol, Coimbatore 641002, Tamil Nadu, India
Reprint Address Veluthakkal, R (reprint author), Inst Forest Genet & Tree Breeding, Div Plant Biotechnol, PB 1061,RS Puram PO, Coimbatore 641002, Tamil Nadu, India.
E-mail Address ghoshm@icfre.org; modhumitaghosh@hotmail.com
Funding Agency and Grant Number Department of Biotechnology, Ministry of Science and Technology Government of India
Funding Text The authors acknowledge Dr R. Viswanathan, Principal Scientist and Head (Plant Protection), Sugarcane Breeding Institute, Coimbatore, India, for providing facilities to conduct RT-qPCR and Dr V. Mohan, Scientist, Division of Forest Protection, Institute of Forest Genetics and Tree Breeding, Coimbatore, India, for providing the fungal strain. The authors thank the Department of Biotechnology, Ministry of Science and Technology Government of India, for the financial support.
Cited References ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999; ARAKI T, 1995, BIOSCI BIOTECH BIOCH, V59, P336; Bailey BA, 2005, PLANT SCI, V168, P1247, DOI 10.1016/j.plantsci.2005.01.002; BAKER CJ, 1995, ANNU REV PHYTOPATHOL, V33, P299, DOI 10.1146/annurev.py.33.090195.001503; Blom N, 1999, J MOL BIOL, V294, P1351, DOI 10.1006/jmbi.1999.3310; BOLLE C, 1994, PLANT J, V6, P513, DOI 10.1046/j.1365-313X.1994.6040513.x; BRADLEY DJ, 1992, CELL, V70, P21, DOI 10.1016/0092-8674(92)90530-P; Bravo JM, 2003, PLANT MOL BIOL, V52, P745, DOI 10.1023/A:1025016416951; BROGLIE K, 1991, SCIENCE, V254, P1194, DOI 10.1126/science.254.5035.1194; BROGLIE KE, 1989, PLANT CELL, V1, P599, DOI 10.1105/tpc.1.6.599; BROGLIE KE, 1986, P NATL ACAD SCI USA, V83, P6820, DOI 10.1073/pnas.83.18.6820; CASPAR T, 1993, PLANT J, V3, P161; Clarke HRG, 1998, PHYSIOL PLANTARUM, V103, P154, DOI 10.1034/j.1399-3054.1998.1030202.x; COLLINGE DB, 1993, PLANT J, V3, P31, DOI 10.1046/j.1365-313X.1993.t01-1-00999.x; DEJONG AJ, 1995, DEV GENET, V16, P332, DOI 10.1002/dvg.1020160406; Dong JZ, 1997, PLANTA, V201, P189, DOI 10.1007/BF01007703; Emanuelsson O, 2000, J MOL BIOL, V300, P1005, DOI 10.1006/jmbi.2000.3903; Floris M, 2009, INT J MOL SCI, V10, P3168, DOI 10.3390/ijms10073168; Fortunato A, 2007, PHYSIOL PLANTARUM, V130, P418, DOI 10.1111/j.1399-3054.2006.00864.x; Fossdal CG, 2006, TREE PHYSIOL, V26, P169; Fraire-Velazquez S, 2011, ABIOTIC STRESS RESPONSE IN PLANTS - PHYSIOLOGICAL, BIOCHEMICAL AND GENETIC PERSPECTIVES, P3; Fujimura T, 2005, CLIN EXP ALLERGY, V35, P234, DOI 10.1111/j.1365-2222.2005.02167.x; GALLOMEAGHER M, 1992, PLANT CELL, V4, P389; Garcia-Casado G, 1998, GLYCOBIOLOGY, V8, P1021, DOI 10.1093/glycob/8.10.1021; GAYNOR JJ, 1988, NUCLEIC ACIDS RES, V16, P5210, DOI 10.1093/nar/16.11.5210; Glazebrook J, 2005, ANNU REV PHYTOPATHOL, V43, P205, DOI 10.1146/annurev.phyto.43.040204.135923; Glazebrook J, 2001, CURR OPIN PLANT BIOL, V4, P301, DOI 10.1016/S1369-5266(00)00177-1; GRAHAM LS, 1994, CAN J BOT, V72, P1057; Gupta R., 2004, PREDICTION N GLYCOSY; Hammond-Kosack KE, 2003, CURR OPIN BIOTECH, V14, P177, DOI 10.1016/S0958-1669(03)00035-1; HENRISSAT B, 1991, BIOCHEM J, V280, P309; Hietala AM, 2004, APPL ENVIRON MICROB, V70, P3948, DOI 10.1128/AEM.70.7.3948-3953.2004; Higo K, 1999, NUCLEIC ACIDS RES, V27, P297, DOI 10.1093/nar/27.1.297; Hong JK, 2002, PHYSIOL PLANTARUM, V114, P549, DOI 10.1034/j.1399-3054.2002.1140407.x; Huang XQ, 1999, GENOME RES, V9, P868, DOI 10.1101/gr.9.9.868; Ibraheem O, 2010, COMPUT BIOL CHEM, V34, P268, DOI 10.1016/j.compbiolchem.2010.09.003; Islam MA, 2010, PHYTOPATHOLOGY, V100, P356, DOI 10.1094/PHYTO-100-4-0356; Johnk N, 2005, TREE PHYSIOL, V25, P1533; Kasprzewska A, 2003, CELL MOL BIOL LETT, V8, P809; KEEN NT, 1983, PLANT PHYSIOL, V71, P460, DOI 10.1104/pp.71.3.460; Kertesz S, 2006, NUCLEIC ACIDS RES, V34, P6147, DOI 10.1093/nar/gkl737; Keulen H, 2008, ENVIRON EXP BOT, V63, P281; Khan AA, 2004, PLANT SCI, V166, P753, DOI 10.1016/j.plantsci.2003.11.015; Kuc J. J., 1985, ARCH BIOCHEM BIOPHYS, V236, P445; Lescot M, 2002, NUCLEIC ACIDS RES, V30, P325, DOI 10.1093/nar/30.1.325; LEVINE A, 1994, CELL, V79, P583, DOI 10.1016/0092-8674(94)90544-4; Liu DL, 2008, J BIOL CHEM, V283, P28010, DOI 10.1074/jbc.M804895200; Liu Jun-Jun, 2005, Phytopathology, V95, P284, DOI 10.1094/PHYTO-95-0284; Livak KJ, 2001, METHODS, V25, P402, DOI 10.1006/meth.2001.1262; Loake G, 2007, CURR OPIN PLANT BIOL, V10, P466, DOI 10.1016/j.pbi.2007.08.008; LUTCKE HA, 1987, EMBO J, V6, P43; MATTON DP, 1993, PLANT MOL BIOL, V22, P279, DOI 10.1007/BF00014935; MAUCH F, 1988, PLANT PHYSIOL, V88, P936, DOI 10.1104/pp.88.3.936; Maximova SN, 2006, PLANTA, V224, P740, DOI 10.1007/s00425-005-0188-6; MELCHERS LS, 1994, PLANT J, V5, P469; Mohan V., 2001, CASUARINA IMPROVEMEN, P171; Mohanan C., 1993, Commonwealth Forestry Review, V72, P48; Mongkolsiriwatana C., 2009, Kasetsart Journal, Natural Sciences, V43, P164; Mur LAJ, 2006, J EXP BOT, V57, P489, DOI 10.1093/jxb/erj052; Neuhaus JM, 1999, PATHOGENESIS-RELATED PROTEINS IN PLANTS, P77; Neuhaus JM, 1996, PLANT MOL BIOL REP, V14, P102, DOI 10.1007/BF02684897; Ohnuma T, 2004, BIOSCI BIOTECH BIOCH, V68, P324, DOI 10.1271/bbb.68.324; Passarinho PA, 2001, PLANTA, V212, P556, DOI 10.1007/s004250000464; Petersen NH, 2011, NAT METHODS, V8, P785; Porat R, 2001, J PLANT PHYSIOL, V158, P1585, DOI 10.1078/0176-1617-00585; Pozo MJ, 2004, J PLANT GROWTH REGUL, V23, P211, DOI [10.1007/s00344-004-003105, 10.1007/s00344-004-0031-5]; Rakwal R, 2004, MOL BIOL REP, V31, P113, DOI 10.1023/B:MOLE.0000031407.18708.95; Rani V, 2007, J BIOSCIENCES, V32, P1325, DOI 10.1007/s12038-007-0142-9; RASMUSSEN U, 1992, PLANT MOL BIOL, V20, P277, DOI 10.1007/BF00014495; RIDE JP, 1975, PHYSIOL PLANT PATHOL, V5, P125, DOI 10.1016/0048-4059(75)90016-8; Rinaldi C, 2007, PLANT PHYSIOL, V144, P347, DOI 10.1104/pp.106.094987; Robert N, 2002, PLANT SCI, V162, P389, DOI 10.1016/S0168-9452(01)00576-3; Rozen S, 2000, Methods Mol Biol, V132, P365; SAHAI AS, 1993, FEMS MICROBIOL REV, V11, P317, DOI 10.1016/0168-6445(93)90004-S; SAMAC DA, 1990, PLANT PHYSIOL, V93, P907, DOI 10.1104/pp.93.3.907; Sambrook J., 1989, MOL CLONING LAB MANU; SCHLUMBAUM A, 1986, NATURE, V324, P365, DOI 10.1038/324365a0; SHAPIRO MB, 1987, NUCLEIC ACIDS RES, V15, P7155, DOI 10.1093/nar/15.17.7155; Sharma J. K., 1994, PATHOLOGICAL INVESTI; Sharma J. K., 1995, TAMP FINL 20 IUFRO W; SHINSHI H, 1990, PLANT MOL BIOL, V14, P357, DOI 10.1007/BF00028772; Shinya T, 2007, BIOCHEM BIOPH RES CO, V353, P311, DOI 10.1016/j.bbrc.2006.12.009; Singh KB, 2002, CURR OPIN PLANT BIOL, V5, P430, DOI 10.1016/S1369-5266(02)00289-3; Somssich IE, 1998, TRENDS PLANT SCI, V3, P86, DOI 10.1016/S1360-1385(98)01199-6; Tamura K, 2011, MOL BIOL EVOL, V28, P2731, DOI 10.1093/molbev/msr121; THOMPSON JD, 1994, NUCLEIC ACIDS RES, V22, P4673, DOI 10.1093/nar/22.22.4673; Titze J. F., 1983, CAS EC MAN UT P INT, P17; VANBUUREN M, 1992, MOL GEN GENET, V232, P460; Van Dahl C. C., 2007, J PLANT GROWTH REGUL, V26, P201; Velazhahan R, 1998, PHYSIOL PLANTARUM, V102, P21, DOI 10.1034/j.1399-3054.1998.1020104.x; Veluthakkal R, 2010, TREES-STRUCT FUNCT, V24, P993, DOI 10.1007/s00468-010-0489-7; Wiweger M, 2003, J EXP BOT, V54, P2691, DOI 10.1093/jxb/erg299; Wu CT, 2003, PLANT PHYSIOL, V133, P263, DOI 10.1104/pp.103.024687; Xiao YH, 2007, BIOSCI BIOTECH BIOCH, V71, P1211, DOI 10.1271/bbb.60658; Zarei A, 2011, PLANT MOL BIOL, V75, P321, DOI 10.1007/s11103-010-9728-y; ZHANG SQ, 1994, PLANT CELL, V6, P135; Zhang SQ, 2001, TRENDS PLANT SCI, V6, P520, DOI 10.1016/S1360-1385(01)02103-3; Zheng HQ, 2009, MOL GENET GENOMICS, V282, P381, DOI 10.1007/s00438-009-0471-5; ZHU Q, 1991, MOL GEN GENET, V226, P289, DOI 10.1007/BF00273615
Cited Reference Count 99
Times Cited 8
Total Times Cited Count (WoS, BCI, and CSCD) 8
Publisher WILEY-BLACKWELL
Publisher City HOBOKEN
Publisher Address 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
ISSN 1437-4781
29-Character Source Abbreviation FOREST PATHOL
ISO Source Abbreviation Forest Pathol.
Publication Date DEC
Year Published 2012
Volume 42
Issue 6
Beginning Page 467
Ending Page 480
Digital Object Identifier (DOI) 10.1111/j.1439-0329.2012.00781.x
Page Count 14
Web of Science Category Forestry
Subject Category Forestry
Document Delivery Number 051PF
Unique Article Identifier WOS:000312138800003
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