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Version 3.22
Publication Type J
Authors Veluthakkal, R; Dasgupta, MG
Author Full Name Veluthakkal, R.; Dasgupta, M. G.
Title Isolation and characterization of pathogen defence-related class I chitinase from the actinorhizal tree Casuarina equisetifolia
Language English
Document Type Article
Abstract Casuarina equisetifolia has the widest distribution of all Casuarina species and is a nitrogen-fixing tree of considerable social, economic and environmental importance. Trichosporium vesiculosum, a causal agent of blister bark disease, is a serious pathogen of C. equisetifolia. In this study, a cDNA clone encoding class I chitinase (CeChi1) belonging to PR-3 family was cloned and characterized from the needle tissues of C. equisetifolia challenged with the toxic exudate of the fungal pathogen T. vesiculosum. The CeChi1 open reading frame comprised 966 nucleotides that encoded 321 amino acid residues with the molecular mass of mature protein being approximately 34 kDa. Analysis of the predicted amino acid sequence revealed the similarity of CeChi1 protein to class I chitinase from other plant species containing a hydrophobic signal peptide domain and hinge domain. The sequence also harboured a cysteine-rich chitin-binding domain and lysozyme-like domain. A hydrophobic C-terminal domain similar to the vacuole targeting sequences of class I chitinases isolated from other plants was also detected. The genomic sequence of CeChi1 indicated that the coding region contained three exons and two introns. In silico analysis of the untranslated regions revealed the presence of several cis-acting regulatory elements associated with hormonal regulation and stress responses. Quantitative real-time PCR analyses at different time points showed upregulation of the transcript during pathogen elicitation and salicylic acid signalling. However, no significant expression of CeChi1 was observed during other abiotic stress condition including wounding, water deficit, salt and heat stress revealing the specific expression of the gene during pathogenesis. This is the first report on isolation of a gene from C. equisetifolia, and the detailed functional analyses of CeChi1 will help in understanding its specific role in defence against pathogens in this tropical tree species.
Author Address [Veluthakkal, R.; Dasgupta, M. G.] Inst Forest Genet & Tree Breeding, Div Plant Biotechnol, Coimbatore 641002, Tamil Nadu, India
Reprint Address Veluthakkal, R (reprint author), Inst Forest Genet & Tree Breeding, Div Plant Biotechnol, PB 1061,RS Puram PO, Coimbatore 641002, Tamil Nadu, India.
E-mail Address ghoshm@icfre.org; modhumitaghosh@hotmail.com
Funding Agency and Grant Number Department of Biotechnology, Ministry of Science and Technology Government of India
Funding Text The authors acknowledge Dr R. Viswanathan, Principal Scientist and Head (Plant Protection), Sugarcane Breeding Institute, Coimbatore, India, for providing facilities to conduct RT-qPCR and Dr V. Mohan, Scientist, Division of Forest Protection, Institute of Forest Genetics and Tree Breeding, Coimbatore, India, for providing the fungal strain. The authors thank the Department of Biotechnology, Ministry of Science and Technology Government of India, for the financial support.
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Cited Reference Count 99
Times Cited 8
Total Times Cited Count (WoS, BCI, and CSCD) 8
Publisher City HOBOKEN
Publisher Address 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
ISSN 1437-4781
29-Character Source Abbreviation FOREST PATHOL
ISO Source Abbreviation Forest Pathol.
Publication Date DEC
Year Published 2012
Volume 42
Issue 6
Beginning Page 467
Ending Page 480
Digital Object Identifier (DOI) 10.1111/j.1439-0329.2012.00781.x
Page Count 14
Web of Science Category Forestry
Subject Category Forestry
Document Delivery Number 051PF
Unique Article Identifier WOS:000312138800003
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