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Authors Ngom, M; Gray, K; Diagne, N; Oshone, R; Fardoux, J; Gherbi, H; Hocher, V; Svistoonoff, S; Laplaze, L; Tisa, LS; Sy, MO; Champion, A
Author Full Name Ngom, Mariama; Gray, Krystelle; Diagne, Nathalie; Oshone, Rediet; Fardoux, Joel; Gherbi, Hassen; Hocher, Valerie; Svistoonoff, Sergio; Laplaze, Laurent; Tisa, Louis S.; Sy, Mame O.; Champion, Antony
Title Symbiotic Performance of Diverse Frankia Strains on Salt-Stressed Casuarina glauca and Casuarina equisetifolia Plants
Source FRONTIERS IN PLANT SCIENCE
Language English
Document Type Article
Author Keywords salinity; Frankia; Casuarina glauca; Casuarina equisetifolia; root hair deformation; CgNIN; Cg12; proline
Keywords Plus ROOT-HAIR INFECTION; NITROGEN-FIXATION; SODIUM-CHLORIDE; ACTINORHIZAL SYMBIOSES; TOLERANT RHIZOBIA; MEDICAGO-SATIVA; N-2 FIXATION; NOD FACTOR; IN-VITRO; NIN GENE
Abstract Symbiotic nitrogen-fixing associations between Casuarina trees and the actinobacteria Frankia are widely used in agroforestry in particular for salinized land reclamation. The aim of this study was to analyze the effects of salinity on the establishment of the actinorhizal symbiosis between C. glauca and two contrasting Frankia strains (salt sensitive; Ccl3 vs. salt tolerant; CeD) and the role of these isolates in the salt tolerance of C. glauca and C. equisetifolia plants. We show that the number of root nodules decreased with increasing salinity levels in both plants inoculated with Ccl3 and CeD. Nodule formation did not occur in seedlings inoculated with Ccl3 and CeD, at NaCl concentrations above 100 and 200 mM, respectively. Salinity also affected the early deformation of plant root hairs and reduced their number and size. In addition, expression of symbiotic marker Cg12 gene, which codes for a subtilase, was reduced at 50 mM NaCl. These data suggest that the reduction of nodulation in C. glauca under salt stress is in part due to inhibition of early mechanisms of infection. We also show that prior inoculation of C. glauca and C. equisetifolia with Frankia strains Ccl3 and CeD significantly improved plant height, dry biomass, chlorophyll and proline contents at all levels of salinity tested, depending on the Casuarina-Frankia association. There was no correlation between in vitro salt tolerance of Frankia strains and efficiency in planta under salt stressed conditions. Our results strongly indicate that increased N nutrition, photosynthesis potential and proline accumulation are important factors responsible for salt tolerance of nodulated C. glauca and C. equisetifolia.
Author Address [Ngom, Mariama; Gray, Krystelle; Diagne, Nathalie; Svistoonoff, Sergio; Laplaze, Laurent; Sy, Mame O.; Champion, Antony] Ctr Rech Bel Air, Lab Mixte Int Adaptat Plantes & Microorganismes A, Dakar, Senegal; [Ngom, Mariama; Sy, Mame O.] Univ Cheikh Anta Diop, Fac Sci & Tech, Dept Biol Vegetale, Lab Campus Biotechnol Vegetales, Dakar, Senegal; [Ngom, Mariama; Gray, Krystelle; Svistoonoff, Sergio; Laplaze, Laurent] Univ Cheikh Anta Diop, Ctr Rech Bel Air, Inst Senegalais Rech Agr, Inst Rech Dev,Lab Commun Microbiol, Dakar, Senegal; [Gray, Krystelle; Laplaze, Laurent; Champion, Antony] Inst Rech Dev, UMR DIADE, Montpellier, France; [Diagne, Nathalie] Inst Senegalais Rech Agr, Ctr Natl Rech Agron, Bambey, Senegal; [Oshone, Rediet; Tisa, Louis S.] Univ New Hampshire, Dept Mol Cellular & Biomed Sci, Durham, NH 03824 USA; [Fardoux, Joel; Gherbi, Hassen; Hocher, Valerie; Svistoonoff, Sergio] Univ Montpellier Sup Agro, Inst Rech Dev INRA CIRAD, Lab Symbioses Trop & Mediterraneennes, Montpellier, France
Reprint Address Champion, A (reprint author), Ctr Rech Bel Air, Lab Mixte Int Adaptat Plantes & Microorganismes A, Dakar, Senegal.; Champion, A (reprint author), Inst Rech Dev, UMR DIADE, Montpellier, France.
E-mail Address antony.champion@ird.fr
ResearcherID Number Laplaze, Laurent/I-5435-2016
ORCID Number Laplaze, Laurent/0000-0002-6568-6504
Funding Agency and Grant Number IRD (Institut de Recherche pour le Developpement); AUF (Agence Universitaire de la Francophonie) through the inter-regional doctoral college in food and plant biotechnology (CD-BIOVEGAGRO); USDA National Institute of Food and Agriculture [Hatch 022821]; USDA AFRI [A1151 2014-03765]; MERS (Ministers de l'Enseignement Superieur et de la Recherche du Senegal); WFS (Word Federation of Scientists); IRD (ARTS PhD grant)
Funding Text This work was supported in part by the IRD (Institut de Recherche pour le Developpement) and the AUF (Agence Universitaire de la Francophonie) through the inter-regional doctoral college in food and plant biotechnology (CD-BIOVEGAGRO) and grants from the USDA National Institute of Food and Agriculture (Hatch 022821) and USDA AFRI (A1151 2014-03765). MN was supported by the MERS (Ministers de l'Enseignement Superieur et de la Recherche du Senegal, national grant), the WFS (Word Federation of Scientists, research allowance) and the IRD (ARTS PhD grant).
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Cited Reference Count 75
Times Cited 1
Total Times Cited Count (WoS, BCI, and CSCD) 1
Publisher FRONTIERS MEDIA SA
Publisher City LAUSANNE
Publisher Address PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND
ISSN 1664-462X
29-Character Source Abbreviation FRONT PLANT SCI
ISO Source Abbreviation Front. Plant Sci.
Publication Date AUG 31
Year Published 2016
Volume 7
Article Number 1331
Digital Object Identifier (DOI) 10.3389/fpls.2016.01331
Page Count 16
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number DU5WK
Unique Article Identifier WOS:000382284000003
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