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Publication Type J
Authors Omoruyi, BE; Bradley, G; Afolayan, AJ
Author Full Name Omoruyi, Beauty E.; Bradley, Graeme; Afolayan, Anthony J.
Title Antioxidant and phytochemical properties of Carpobrotus edulis (L.) bolus leaf used for the management of common infections in HIV/AIDS patients in Eastern Cape Province
Source BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
Language English
Document Type Article
Author Keywords Carpobrotus edulis; Solvent extraction; Antioxidant; Free radicals; Phytoconstituents
Keywords Plus POLYPHENOL CONTENTS; EXTRACTS; RATS
Abstract Background: Carpobrotus edulis (Mesembryanthemaceae), also known as igcukuma in Xhosa language is a medicinal plant used by the traditional healers to treat common infections in HIV/AIDS patients. Based on this information, we researched on the plant phytoconstituents, as well as its inhibitory effect using aqueous and three different organic solvent extracts in order to justify its therapeutic usage. Methods: Antioxidant activity of the extracts were investigated spectrophotometrically against 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) diammonium salt, hydrogen peroxide (H2O2), nitric oxide (NO), and ferric reducing power, Total phenols, flavonoids, flavonols, proanthocyanidins, tannins, alkaloids and saponins were also determined using the standard methods. Results: Quantitative phytochemical analysis of the four solvent extracts revealed a high percentage of phenolics (55.7 +/- 0.404%) in the acetone extract, with appreciable amount of proanthocyanidins (86.9 +/- 0.005%) and alkaloids (4.5 +/- 0.057%) in the aqueous extract, while tannin (48.9 +/- 0.28%) and saponin (4.5 +/- 0.262%) were major constituents of the ethanol extract. Flavonoids (0.12 +/- 0.05%) and flavonols (0.12 +/- 0.05%) were found at higher level in the hexane extract in comparison with the other extracts. The leaf extracts demonstrated strong hydrogen peroxide scavenging activity, with the exception of water and ethanol extracts. IC50 values of the aqueous and ethanolic extract against DPPH, ABTS, and NO were 0.018 and 0.016; 0.020 and 0.022; 0.05 and 0.023 mg/ml, respectively. The reducing power of the extract was found to be concentration dependent. Conclusion: The inhibitory effect of the extracts on free radicals may justify the traditional use of this plant in the management of common diseases in HIV/AIDs patients in Eastern Cape Province. Overall, both aqueous and ethanol were found to be the best solvents for antioxidant activity in C. edulis leaves.
Author Address [Afolayan, Anthony J.] Univ Ft Hare, Dept Bot, ZA-5700 Alice, South Africa; [Omoruyi, Beauty E.; Bradley, Graeme] Univ Ft Hare, Dept Biochem & Microbiol, ZA-5700 Alice, South Africa
Reprint Address Afolayan, AJ (reprint author), Univ Ft Hare, Dept Bot, Private Bag X1314, ZA-5700 Alice, South Africa.
E-mail Address aafolayan@ufh.ac.za
ResearcherID Number Bradley, Graeme/B-8604-2009
ORCID Number Bradley, Graeme/0000-0002-9036-9869
Funding Agency and Grant Number National Research Foundation of South Africa; Govan Mbeki Research and Development centre of the University of Fort Hare
Funding Text This work was supported by the National Research Foundation of South Africa and the Govan Mbeki Research and Development centre of the University of Fort Hare.
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Cited Reference Count 45
Times Cited 12
Total Times Cited Count (WoS, BCI, and CSCD) 12
Publisher BIOMED CENTRAL LTD
Publisher City LONDON
Publisher Address 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND
ISSN 1472-6882
29-Character Source Abbreviation BMC COMPLEM ALTERN M
ISO Source Abbreviation BMC Complement. Altern. Med.
Publication Date NOV 9
Year Published 2012
Volume 12
Article Number 215
Digital Object Identifier (DOI) 10.1186/1472-6882-12-215
Page Count 9
Web of Science Category Integrative & Complementary Medicine
Subject Category Integrative & Complementary Medicine
Document Delivery Number 059SN
Unique Article Identifier WOS:000312725700002
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