eHALOPH - Halophytes Database

Publication Type	J
Authors	Omoruyi, BE; Bradley, G; Afolayan, AJ
Author Full Name	Omoruyi, Beauty E.; Bradley, Graeme; Afolayan, Anthony J.
Title	Antioxidant and phytochemical properties of Carpobrotus edulis (L.) bolus leaf used for the management of common infections in HIV/AIDS patients in Eastern Cape Province
Source	BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
Language	English
Document Type	Article
Author Keywords	Carpobrotus edulis; Solvent extraction; Antioxidant; Free radicals; Phytoconstituents
Keywords Plus	POLYPHENOL CONTENTS; EXTRACTS; RATS
Abstract	Background: Carpobrotus edulis (Mesembryanthemaceae), also known as igcukuma in Xhosa language is a medicinal plant used by the traditional healers to treat common infections in HIV/AIDS patients. Based on this information, we researched on the plant phytoconstituents, as well as its inhibitory effect using aqueous and three different organic solvent extracts in order to justify its therapeutic usage. Methods: Antioxidant activity of the extracts were investigated spectrophotometrically against 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) diammonium salt, hydrogen peroxide (H2O2), nitric oxide (NO), and ferric reducing power, Total phenols, flavonoids, flavonols, proanthocyanidins, tannins, alkaloids and saponins were also determined using the standard methods. Results: Quantitative phytochemical analysis of the four solvent extracts revealed a high percentage of phenolics (55.7 +/- 0.404%) in the acetone extract, with appreciable amount of proanthocyanidins (86.9 +/- 0.005%) and alkaloids (4.5 +/- 0.057%) in the aqueous extract, while tannin (48.9 +/- 0.28%) and saponin (4.5 +/- 0.262%) were major constituents of the ethanol extract. Flavonoids (0.12 +/- 0.05%) and flavonols (0.12 +/- 0.05%) were found at higher level in the hexane extract in comparison with the other extracts. The leaf extracts demonstrated strong hydrogen peroxide scavenging activity, with the exception of water and ethanol extracts. IC50 values of the aqueous and ethanolic extract against DPPH, ABTS, and NO were 0.018 and 0.016; 0.020 and 0.022; 0.05 and 0.023 mg/ml, respectively. The reducing power of the extract was found to be concentration dependent. Conclusion: The inhibitory effect of the extracts on free radicals may justify the traditional use of this plant in the management of common diseases in HIV/AIDs patients in Eastern Cape Province. Overall, both aqueous and ethanol were found to be the best solvents for antioxidant activity in C. edulis leaves.
Author Address	[Afolayan, Anthony J.] Univ Ft Hare, Dept Bot, ZA-5700 Alice, South Africa; [Omoruyi, Beauty E.; Bradley, Graeme] Univ Ft Hare, Dept Biochem & Microbiol, ZA-5700 Alice, South Africa
Reprint Address	Afolayan, AJ (reprint author), Univ Ft Hare, Dept Bot, Private Bag X1314, ZA-5700 Alice, South Africa.
E-mail Address	aafolayan@ufh.ac.za
ResearcherID Number	Bradley, Graeme/B-8604-2009
ORCID Number	Bradley, Graeme/0000-0002-9036-9869
Funding Agency and Grant Number	National Research Foundation of South Africa; Govan Mbeki Research and Development centre of the University of Fort Hare
Funding Text	This work was supported by the National Research Foundation of South Africa and the Govan Mbeki Research and Development centre of the University of Fort Hare.
Cited References	Alex B., 2012, AM J PHARM TOXICOL, V7, P80; Ashafa AOT, 2011, AFR J PHARM PHARMACO, V5, P150; Banerjee S., 2011, Asian Journal of Experimental Biological Sciences, V2, P595; Brenes M, 2000, J AGR FOOD CHEM, V48, P5178, DOI 10.1021/jf000686e; Bursal E, 2011, FOOD RES INT, V44, P1482, DOI 10.1016/j.foodres.2011.03.031; Bushra Sultana, 2009, Molecules, V14, P2167, DOI 10.3390/molecules14062167; Cetinkaya Y, 2012, ARCH PHARM, V345, P323, DOI 10.1002/ardp.201100272; Ebrahimzadeh MA, 2010, PAK J PHARM SCI, V23, P29; Ewa F., 2009, PHARM REPORT, V61, P935; Fang YZ, 2002, NUTRITION, V18, P872, DOI 10.1016/S0899-9007(02)00916-4; Gocer H, 2011, INT J FOOD SCI NUTR, V62, P821, DOI 10.3109/09637486.2011.585963; Gulcin I, 2012, ARCH TOXICOL, V86, P345, DOI 10.1007/s00204-011-0774-2; Gulcin I, 2011, J MED FOOD, V14, P975, DOI 10.1089/jmf.2010.0197; Gulcin I, 2011, J FOOD SCI, V76, pC585, DOI 10.1111/j.1750-3841.2011.02142.x; Gulcin I, 2011, REC NAT PROD, V5, P158; Hanen F, 2011, IND CROP PROD, V34, P1066; Hashasa MM, 2010, ACTA PHARM, V60, P361; Hemalatha S, 2011, J CHEM PHARM RES, V3, P259; Karunakaran RJ, 2007, LWT, V40, P344; Kirmizigul S, 2007, PURE APPL CHEM, V79, P2297, DOI 10.1351/pac200779122297; Liyana-Pathirana CM, 2005, J AGR FOOD CHEM, V53, P2433, DOI 10.1021/jf049320i; Madhavi D. L., 1995, TOXICOLOGICAL ASPECT, P267; [Anonymous], 2009, INT J INTEGR BIOL; Mbaebie B. O., 2012, Asian Pacific Journal of Tropical Biomedicine, V2, P118, DOI 10.1016/S2221-1691(11)60204-9; Mohammed H.M., 2008, J SCI FOOD AGR, V89, P897; Mohammed F, 2010, AFR J BIOTECHNOL, V9, P326; Ndip Roland N, 2007, Afr Health Sci, V7, P228; Nihal TY., 2007, MOLECULES, V12, P484; Noha A., 2011, WORLD ACAD SCI ENGIN, V75, P86; Okwu DE, 2006, AFR J BIOTECHNOL, V5, P357; Olubunmi A.W., 2011, PHARMACOGN MAG, V7, P325; Onyilagha JC, 2009, INT J AGRIC BIOL, V11, P231; Ordonez AAL, 2006, FOOD CHEM, V97, P452, DOI 10.1016/j.foodchem.2005.05.024; Park EJ, 2010, LWT-FOOD SCI TECHNOL, V43, P655, DOI 10.1016/j.lwt.2009.11.005; PDR Health - Physicians Desktop Reference, HERB SUPPL RUT DRUGS; Pirwana K, 2008, AFRICAN J BIOTECH, V7, P4164; Re R, 1999, FREE RADICAL BIO MED, V26, P1231, DOI 10.1016/S0891-5849(98)00315-3; Shu-Dong W, 2010, INT J MOL SCI, V11, P4080; Smith-Palmer A, 1998, LETT APPL MICROBIOL, V26, P118, DOI 10.1046/j.1472-765X.1998.00303.x; Sun J, 2002, J AGR FOOD CHEM, V50, P7449, DOI 10.1021/jf0207530; Syeda B, 2008, PAK J ANAL ENV CHEM, V9, P78; Traber M, 2006, MODERN NUTR HLTH DIS, P396; Yang CS, 2001, ANNU REV NUTR, V21, P381, DOI 10.1146/annurev.nutr.21.1.381; Yazdanparast R, 2008, CHEM-BIOL INTERACT, V172, P176, DOI 10.1016/j.cbi.2008.01.006; Zovko K. M., 2010, FOOD CHEM TOXICOL, V48, P1537, DOI [10.1016/j.fct.2010.03.02120304028, DOI 10.1016/J.FCT.2010.03.021]
Cited Reference Count	45
Times Cited	12
Total Times Cited Count (WoS, BCI, and CSCD)	12
Publisher	BIOMED CENTRAL LTD
Publisher City	LONDON
Publisher Address	236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND
ISSN	1472-6882
29-Character Source Abbreviation	BMC COMPLEM ALTERN M
ISO Source Abbreviation	BMC Complement. Altern. Med.
Publication Date	NOV 9
Year Published	2012
Volume	12
Article Number	215
Digital Object Identifier (DOI)	10.1186/1472-6882-12-215
Page Count	9
Web of Science Category	Integrative & Complementary Medicine
Subject Category	Integrative & Complementary Medicine
Document Delivery Number	059SN
Unique Article Identifier	WOS:000312725700002
Plants associated with this reference	Carpobrotus edulis