Loading content, please wait..
loading..
Logo
Version 3.18
or
Publication Type J
Authors Fan, W., W. J. Chang, X. W. Liu, C. Xiao, J. L. Yang and Z. L. Zhang
Title Identification of up-regulated genes provides integrated insight into salt-induced tolerance mechanisms in Sesuvium portulacastrum roots
Source Acta Physiologiae Plantarum
Author Keywords Sesuvium portulacastrum Suppression subtractive hybridization mRNA differential expression profiles Salt tolerance crassulacean-acid-metabolism activated protein-kinase abiotic stress tolerance common ice plant mesembryanthemum-crystallinum transgenic tobacco salinity tolerance proteomic analysis responsive genes mangrove plant
Abstract Sesuvium portulacastrum, a promising facultative halophyte species, has a remarkable ability to survival at severe salt of similar to 1000 mM NaCl. However, genes encoding proteins involved in the process of salt tolerance in S. portulacastrum are limited. In this study, suppression subtractive hybridization together with reverse northern blot analysis and RT-PCR was used to identify up-regulated genes in roots after treatment with seawater for 2-24 h. We identified a total of 148 unigenes, and most of these genes were predominantly associated with 'signal transduction and transcription', 'stress/defense response', 'transport' and 'metabolism'. Comparative analysis between physiological response and differentially expressed genes highlighted that sodium (Na+) transport, compartmentation, reactive oxygen species (ROS) scavenging, and sugar-metabolizing regulation play important roles in salt tolerance of S. portulacastrum, whereas inhibition of root elongation induced by high salinity may be related to lignin deposition and disruption of calcium homeostasis.
Author Address [Fan, Wei] Yunnan Agr Univ, Coll Resources & Environm, Kunming 650201, Peoples R China. [Fan, Wei; Chang, Wenjun; Liu, Xiwen; Zhang, Zhili] Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab, Minist Agr Trop Biotechnol, Haikou 571101, Peoples R China. [Zhang, Zhili] Hainan Acad Agr Sci, Haikou 571100, Peoples R China. [Xiao, Chuan] Huazhong Univ Sci & Technol, Inst Resource Biol & Biotechnol, Dept Biotechnol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China. [Yang, Jianli] Zhejiang Univ, State Key Lab Plant Physiol & Biochem, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China. Zhang, ZL (reprint author), Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab, Minist Agr Trop Biotechnol, Haikou 571101, Peoples R China.; Zhang, ZL (reprint author), Hainan Acad Agr Sci, Haikou 571100, Peoples R China. fanwei1128@aliyun.com; cularmy@163.com; liuxiwen624716541@163.com; xiaochuanapple@163.com; yangjianli@zju.edu.cn; zzl_haas@163.com
ISSN 0137-5881
ISBN 0137-5881
29-Character Source Abbreviation Acta Physiol. Plant.
Publication Date Mar
Year Published 2017
Volume 39
Issue 3
Digital Object Identifier (DOI) 10.1007/s11738-017-2383-z
Unique Article Identifier WOS:000396112600021
Plants associated with this reference

LEGAL NOTICES — This website is protected by Copyright © The University of Sussex, 2012, 2013, 2014, 2015, 2016, 2017, 2018. The eHALOPH database is protected by Database Right and Copyright © The University of Sussex and other contributors, 2006, 2012, 2013, 2014, 2015, 2016, 2017, 2018. This database is based on an earlier work by James Aronson.
THIS WEBSITE AND THIS DATABASE ARE PROVIDED ON AN "AS IS" BASIS, AND YOU USE THEM AND RELY ON THEM AT YOUR OWN RISK.

Contact email: halophytes@sussex.ac.uk
Credits – Tim Flowers, Joaquim Santos, Moritz Jahns, Brian Warburton, Peter Reed