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Publication Type J
Authors Suzuki-Yamamoto, M; Mimura, T; Ashihara, H
Author Full Name Suzuki-Yamamoto, Mihoko; Mimura, Tetsuro; Ashihara, Hiroshi
Title Effect of short-term salt stress on the metabolic profiles of pyrimidine, purine and pyridine nucleotides in cultured cells of the mangrove tree, Bruguiera sexangula
Source PHYSIOLOGIA PLANTARUM
Language English
Document Type Article
Keywords Plus AVICENNIA-MARINA; XANTHINE DEHYDROGENASE; COMPATIBLE SOLUTES; ALDEHYDE OXIDASE; SALVAGE PATHWAYS; ADENOSINE KINASE; COFFEA-ARABICA; INORGANIC-IONS; HIGHER-PLANTS; DE-NOVO
Abstract To investigate the short-term (3h) effect of salt on the metabolism of purine, pyrimidine and pyridine nucleotides in mangrove (Bruguiera sexangula) cells, we examined the uptake and overall metabolism of radiolabelled intermediates involved in the de novo pathways and substrates of salvage pathways for nucleotide biosynthesis in the presence and absence of 100 mMNaCl. Uptake by the cells of substrates for the salvage pathways was much faster than uptake of intermediates of the de novo pathways. The activity of the de novo pyrimidine biosynthesis estimated by [2-(14) C]orotate metabolism was not significantly affected by the salt. About 20-30% of [2-C-14]uridine, [2-C-14]uracil and more than 50% of [2-C-14]cytidine were salvaged for pyrimidine nucleotide biosynthesis. However, substantial quantities of these compounds were degraded to (CO2)-C-14 via beta-ureidopropionate (beta-UP), and degradation of beta-UP was increased by the salt. The activities of the de novo pathway, estimated by [2-C-14] 5-aminoimidazole-4-carboxamide ribonucleoside, and the salvage pathways from [8-C-14]adenosine and [8-C-14]guanosine for the purine nucleotide biosynthesis were not influenced by the salt. Most [8-C-14]hypoxanthine was catabolised to (CO2)-C-14, and other purine compounds are also catabolised via xanthine. Purine catabolism was stimulated by the salt. [H-3] Quinolinate, [carbonyl-C-14]nicotinamide and [carboxyl-C-14]nicotinic acid were utilised for the biosynthesis of pyridine nucleotides. The salvage pathways for pyridine nucleotides were significantly stimulated by the salt. Trigonelline was synthesised from all pyridine precursors that were examined; its synthesis was also stimulated by the salt. We discuss the physiological role of the salt-stimulated reactions of nucleotide metabolism.
Author Address Ochanomizu Univ, Grad Sch Human & Sci, Bunkyo Ku, Tokyo 1128610, Japan; Kobe Univ, Fac Sci, Dept Biol, Nada Ku, Kobe, Hyogo 6578501, Japan; Ochanomizu Univ, Fac Sci, Dept Biol, Bunkyo Ku, Tokyo 1128610, Japan
Reprint Address Ashihara, H (reprint author), Ochanomizu Univ, Grad Sch Human & Sci, Bunkyo Ku, Tokyo 1128610, Japan.
E-mail Address ashihara@cc.ocha.ac.jp
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Cited Reference Count 56
Times Cited 10
Total Times Cited Count (WoS, BCI, and CSCD) 11
Publisher BLACKWELL PUBLISHING
Publisher City OXFORD
Publisher Address 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND
ISSN 0031-9317
29-Character Source Abbreviation PHYSIOL PLANTARUM
ISO Source Abbreviation Physiol. Plant.
Publication Date NOV
Year Published 2006
Volume 128
Issue 3
Beginning Page 405
Ending Page 414
Digital Object Identifier (DOI) 10.1111/j.1399-3054.2006.00753.x
Page Count 10
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number 096PT
Unique Article Identifier WOS:000241390000003
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