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Publication Type J
Authors Tuo, L; Li, J; Liu, SW; Liu, Y; Hu, L; Chen, L; Jiang, MG; Sun, CH
Author Full Name Tuo, Li; Li, Jing; Liu, Shao-Wei; Liu, Yang; Hu, Lin; Chen, Li; Jiang, Ming-Guo; Sun, Cheng-Hang
Title Microlunatus endophyticus sp nov., an endophytic actinobacterium isolated from bark of Bruguiera sexangula
Source INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Language English
Document Type Article
Keywords Plus DEOXYRIBONUCLEIC-ACID; MAXIMUM-LIKELIHOOD; GINSENG FIELD; SOIL; BACTERIUM; SEQUENCES; CHINA; RATES
Abstract A Gram-stain-positive, aerobic, coccoid, non-motile, non-spore-forming bacterium, designated strain S3Af-1(T), was isolated from surface-sterilized bark of Bruguiera sexangula collected from Dongzhaigang National Nature Reserve in Hainan, China, and examined using a polyphasic approach to clarify its taxonomic position. This bacterium did not produce substrate mycelia or aerial hyphae, and no diffusible pigments were observed on the media tested. Strain S3Af-1(T) grew optimally without NaCl, at 28-30 degrees C and at pH 7.0.Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S3Af-1(T) belonged to the genus Microlunatus and shared highest similarity with `Microlunatus terrae' BS6 (97.43 %) and Microlunatus soli CC-12602(T) (97.08 %). DNA DNA hybridization results indicated that the level of relatedness between strain S3Af-1(T) and M. soli CC-12602(T) was less than 70 %. The DNA G+C content of strain S3Af-1(T) was 67.1 mol%. The cell-wall peptidoglycan contained LL-2,6-diaminopimelic acid. MK-9(H-6) and MK-9(H4) were the predominant menaquinones. Phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid, two unidentified phospholipids and other lipids were detected in the polar lipid extracts. The major fatty acids were iso-C-16:0, anteiso-C-17:0 and anteiso-C-15:0. On the basis of phylogenetic analysis, and phenotypic and chemotaxonomic characteristics, strain S3Af-1(T) represents a novel species of the genus Microlunatus, for which the name Micrblunatus endophyticus sp. nov. is proposed. The type strain is S3Af-1(T) (=DSM 100019(T)=CGMCC 4.7306(T)).
Author Address [Tuo, Li] Zunyi Med Univ, Res Ctr Med & Biol, Zunyi 563003, Peoples R China; [Tuo, Li; Li, Jing; Liu, Shao-Wei; Sun, Cheng-Hang] Chinese Acad Med Sci, Inst Med Biotechnol, Beijing 100050, Peoples R China; [Tuo, Li; Li, Jing; Liu, Shao-Wei; Sun, Cheng-Hang] Peking Union Med Coll, Beijing 100050, Peoples R China; [Liu, Shao-Wei; Jiang, Ming-Guo] Guangxi Univ Nationalities, Guangxi Coll & Univ Key Lab Utilizat Microbial &, Nanning 530006, Peoples R China; [Liu, Yang] China Pharmaceut Univ, Coll Life Sci & Technol, Nanjing 210009, Jiangsu, Peoples R China; [Hu, Lin; Chen, Li] Chinese Acad Sci, Inst Zool, Beijing 100101, Peoples R China
Reprint Address Sun, CH (reprint author), Chinese Acad Med Sci, Inst Med Biotechnol, Beijing 100050, Peoples R China.; Sun, CH (reprint author), Peking Union Med Coll, Beijing 100050, Peoples R China.
E-mail Address chenghangsun@hotmail.com
Funding Agency and Grant Number National Natural Sciences Foundation of China (NSFC) [81172963, 31260004, 81321004]; National Science and Technology Major Project from the Ministry of Science and Technology of China [2012ZX09301-002-001-018]
Funding Text We are grateful to Cai-Rong Zhong from Hai Nan Dong Zhai Gang National Nature Reserve Authority for his assistance with collecting samples. This research was partly supported by the National Natural Sciences Foundation of China (NSFC, Grant nos. 81172963,31260004 and 81321004) and the National Science and Technology Major Project (Grant no. 2012ZX09301-002-001-018) from the Ministry of Science and Technology of China.
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Cited Reference Count 34
Times Cited 1
Total Times Cited Count (WoS, BCI, and CSCD) 2
Publisher SOC GENERAL MICROBIOLOGY
Publisher City READING
Publisher Address MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND
ISSN 1466-5026
29-Character Source Abbreviation INT J SYST EVOL MICR
ISO Source Abbreviation Int. J. Syst. Evol. Microbiol.
Publication Date JAN
Year Published 2016
Volume 66
Part Number 1
Beginning Page 481
Ending Page 486
Digital Object Identifier (DOI) 10.1099/ijsem.0.000779
Page Count 6
Web of Science Category Microbiology
Subject Category Microbiology
Document Delivery Number DF6GC
Unique Article Identifier WOS:000371452900069
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