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Publication Type J
Authors Parida, AK; Das, AB; Mohanty, P
Author Full Name Parida, AK; Das, AB; Mohanty, P
Title Investigations on the antioxidative defence responses to NaCl stress in a mangrove, Bruguiera parviflora: Differential regulations of isoforms of some antioxidative enzymes
Source PLANT GROWTH REGULATION
Language English
Document Type Article
Author Keywords antioxidative enzymes; Bruguiera parviflora; hydroponic culture; lipid peroxidation; mangrove; sodium chloride
Keywords Plus CHILLING-SENSITIVE PLANTS; SUPEROXIDE-DISMUTASE; SALT STRESS; GLUTATHIONE-REDUCTASE; LIPID-PEROXIDATION; TEMPERATURE STRESS; HYDROGEN-PEROXIDE; SALINITY STRESS; LEAVES; CATALASE
Abstract Two-month-old healthy seedlings of a true mangrove, Bruguiera parviflora, raised from propagules in normal nursery conditions were subjected to varying concentrations of NaCl for 45 d under hydroponic culture conditions to investigate the defence potentials of antioxidative enzymes against NaCl stress imposed oxidative stress. Changes in the activities of the antioxidative enzymes catalase ( CAT), ascorbate peroxidase (APX), guaiacol peroxidase ( POX), glutathione reductase (GR) and superoxide dismutase ( SOD) were assayed in leaves to monitor the temporal regulation. Among the oxidative stress triggered chemicals, the level of H2O2 was significantly increased while total ascorbate and total glutathione content decreased. The ratio of reduced to oxidized glutathiones, however, increased due to decreased levels of oxidized glutathione in the leaf tissue. Among the five antioxidative enzymes monitored, the APX, POX, GR and SOD specific activities were significantly enhanced at high concentration (400 mM NaCl), while the catalase activities declined, suggesting both up and downregulations of antioxidative enzymes occurred due to NaCl imposed osmotic and ionic stress. Analysis of the stress induced alterations in the isoforms of CAT, APX, POX, GR and SOD revealed differential regulations of the isoforms of these enzymes. In B. parviflora one isoform of each of Mn-SOD and Cu/Zn-SOD while three isoforms of Fe-SOD were observed by activity staining gel. Of these, only Mn-SOD and Fe-SOD2 content was preferentially elevated by NaCl treatment, whereas isoforms of Cu/Zn-SOD, Fe-SOD1 and Fe-SOD3 remained unchanged. Similarly, out of the six isoforms of POX, the POX-1,-2,-3 and -6 were enhanced due to salt stress but the levels of POX-4 and -5 remained same as in control plants suggesting preferential upregulation of selective POX isoforms. Activity staining gel revealed only one prominent band of APX and this band increased with increased salt concentration. Similarly, two isoforms of GR (GR1 and GR2) were visualized on activity staining gel and both these isoforms increased upon salt stress. In this mangrove four CAT-isoforms were identified, among which the prominent CAT-2 isoform level was maximally reduced again suggesting differential downregulation of CAT isoforms by NaCl stress. The results presented in this communication are the first report on the resolutions of isoforms APX, POX and GR out of five antioxidative enzymes studied in the leaf tissue of a true mangrove. The differential changes in the levels of the isoforms due to NaCl stress may be useful as markers for recognizing salt tolerance in mangroves. Further, detailed analysis of the isoforms of these antioxidative enzymes is required for using the various isoforms as salt stress markers. Our results indicate that the overproduction of H2O2 by NaCl treatment functions as a signal of salt stress and causes upregulation of APX, POX, GR and deactivations of CAT in B. parviflora. The concentrations of malondialdehyde, a product of lipid peroxidation and lipoxygenase activity remained unchanged in leaves treated with different concentrations of NaCl, which again suggests that the elevated levels of the antioxidant enzymes protect the plants against the activated oxygen species thus avoiding lipid peroxidation during salt stress.
Author Address Natl Inst Plant Biodivers Conservat, Bhubaneswar 751015, Orissa, India; Reg Plant Resource Ctr, Bhubaneswar 751015, Orissa, India
Reprint Address Das, AB (reprint author), Natl Inst Plant Biodivers Conservat, Bhubaneswar 751015, Orissa, India.
E-mail Address a_b_das@hotmail.com
ResearcherID Number PARIDA, ASISH KUMAR/C-4771-2009
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Cited Reference Count 50
Times Cited 31
Total Times Cited Count (WoS, BCI, and CSCD) 39
Publisher KLUWER ACADEMIC PUBL
Publisher City DORDRECHT
Publisher Address VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
ISSN 0167-6903
29-Character Source Abbreviation PLANT GROWTH REGUL
ISO Source Abbreviation Plant Growth Regul.
Publication Date MAR
Year Published 2004
Volume 42
Issue 3
Beginning Page 213
Ending Page 226
Digital Object Identifier (DOI) 10.1023/B:GROW.0000026508.63288.39
Page Count 14
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number 817WW
Unique Article Identifier WOS:000221208600003
Plants associated with this reference

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