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Version 3.18
Publication Type J
Authors Parida, AK; Das, AB; Mohanty, P
Author Full Name Parida, AK; Das, AB; Mohanty, P
Title Defense potentials to NaCl in a mangrove, Bruguiera parviflora: Differential changes of isoforms of some antioxidative enzymes
Language English
Document Type Article
Author Keywords antioxidative enzymes; Bruguiera parviflora; hydroponic culture; lipid peroxidation; mangrove; sodium chloride
Abstract In order to assess the role of the antioxidative defense system against salt treatment, the activities of some antioxidative enzymes and levels of antioxidants were monitored in a true mangrove, Bruguiera parviflora, subjected to varying levels of NaCl under hydroponic culture. In the leaves of B. parviflora, salt treatment preferentially enhanced the content of H2O2 as well as the activity of ascorbate peroxidase (APX), guaiacol peroxidase (GPX), glutathione reductase (GR), and superoxide dismutase (SOD), whereas it induced the decrease of total ascorbate and glutathione (GSH+GSSG) content as well as catalase (CAT) activity. Analysis of isoforms of antioxidative enzymes by native PAGE and activity staining revealed that leaves of B. parviflora had one isoform each of Mn-SOD and Cu/ZnSOD and three isoforms of Fe-SOD. Expression of Mn-SOD and Fe-SOD-2 was preferentially elevated by NaCl. Similarly, out of the six isoforms of GPX, the GPX-1, 2, 3 and 6 were enhanced by salt treatment but the levels of GPX-4 and -5 changed minimally as compared to those of a control. Activity staining gel revealed only one prominent isoform of APX and two isoforms of GR (GR-1 and GR-2), all of these isoforms increased upon salt exposure. Four CAT-isoforms were identified, among which the prominent CAT-2 isoform level was maximally reduced, suggesting differential down regulation of CAT isoforms by NaCl. The concentrations of malondialdehyde (MDA), a product of lipid peroxidation, remained unchanged in leaves of the plant treated with different concentrations of NaCl. This suggests that plants are protected against activated oxygen species by the elevated levels of certain antioxidative enzymes, thus avoiding lipid peroxidation during salt exposure. The differential changes in the levels of the isoforms due to NaCl treatment may be useful as markers for recognizing salt tolerance in mangroves.
Author Address Natl Inst Plant Biodivers Conservt & Res, Bhubaneswar 751015, Orissa, India; Reg Plant Resource Ctr, Bhubaneswar 751015, Orissa, India
Reprint Address Das, AB (reprint author), Natl Inst Plant Biodivers Conservt & Res, Bhubaneswar 751015, Orissa, India.
E-mail Address a_b_das@hotmail.com
ResearcherID Number PARIDA, ASISH KUMAR/C-4771-2009
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Cited Reference Count 56
Times Cited 135
Total Times Cited Count (WoS, BCI, and CSCD) 157
Publisher City JENA
Publisher Address BRANCH OFFICE JENA, P O BOX 100537, D-07705 JENA, GERMANY
ISSN 0176-1617
29-Character Source Abbreviation J PLANT PHYSIOL
ISO Source Abbreviation J. Plant Physiol.
Publication Date MAY
Year Published 2004
Volume 161
Issue 5
Beginning Page 531
Ending Page 542
Digital Object Identifier (DOI) 10.1078/0176-1617-01084
Page Count 12
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number 823LI
Unique Article Identifier WOS:000221613300004
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