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Publication Type J
Authors Parida, AK; Das, AB; Mittra, B; Mohanty, P
Author Full Name Parida, AK; Das, AB; Mittra, B; Mohanty, P
Title Salt-stress induced alterations in protein profile and protease activity in the mangrove Bruguiera parviflora
Source ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES
Language English
Document Type Article
Author Keywords hydroponic; polypeptide; protease
Keywords Plus MOLECULAR RESPONSES; RAPHANUS-SATIVUS; WATER-DEFICIT; NACL; GYMNORRHIZA; TOLERANT; SALINITY; LEAVES; ROOTS
Abstract Two-month-old seedlings of Bruguiera parvifora were treated with varying levels of NaCl (100, 200 and 400 mm) under hydroponic culture. Total proteins were extracted from leaves of control and NaCl treated plants after 7, 14, 30 and 45 d of treatment and analysed by SDS-PAGE. As visualized from SDS-PAGE, the intensity of several protein bands of molecular weight 17, 23, 32, 33 and 34 kDa decreased as a result of NaCl treatment. The degree of decrease of these protein bands seemed to be roughly proportional to the external NaCl concentration. The most obvious change concerned a 23 kDa-polypeptide (SSP-23), which disappeared after 45 d treatment in 400 mm NaCl. Moreover, the SSP-23 protein, which disappeared in B. parviflora under salinity stress, reappeared when these salinized seedlings were desalinized. These observations suggest the possible involvement of these polypeptides for osmotic adjustment under salt stress. NaCl stress also caused an increase in the activity of both acid and alkaline protease. The increasing activity of proteases functions as a signal of salt stress in B. parviflora, which induces the reduction of protein level.
Author Address Natl Inst Plant Biodivers Conservat & Res, Bhubaneswar 751015, Orissa, India; Reg Plant Resource Ctr, Bhubaneswar 751015, Orissa, India; Univ Delhi, Sch Environm Studies, Ctr Environm Management Degraded Ecosyst, Delhi 110007, India
Reprint Address Parida, AK (reprint author), Natl Inst Plant Biodivers Conservat & Res, Bhubaneswar 751015, Orissa, India.
E-mail Address asishparida2003@yahoo.com
ResearcherID Number PARIDA, ASISH KUMAR/C-4771-2009
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Cited Reference Count 30
Times Cited 32
Total Times Cited Count (WoS, BCI, and CSCD) 34
Publisher VERLAG Z NATURFORSCH
Publisher City TUBINGEN
Publisher Address POSTFACH 2645, W-7400 TUBINGEN, GERMANY
ISSN 0939-5075
29-Character Source Abbreviation Z NATURFORSCH C
ISO Source Abbreviation Z.Naturforsch.(C)
Publication Date MAY-JUN
Year Published 2004
Volume 59
Issue 5-6
Beginning Page 408
Ending Page 414
Page Count 7
Web of Science Category Biochemistry & Molecular Biology; Pharmacology & Pharmacy
Subject Category Biochemistry & Molecular Biology; Pharmacology & Pharmacy
Document Delivery Number 840NU
Unique Article Identifier WOS:000222866600022
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