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Publication Type J
Authors Parida, AK; Mittra, B; Das, AB; Das, TK; Mohanty, P
Author Full Name Parida, AK; Mittra, B; Das, AB; Das, TK; Mohanty, P
Title High salinity reduces the content of a highly abundant 23-kDa protein of the mangrove Bruguiera parviflora
Source PLANTA
Language English
Document Type Article
Author Keywords Bruguiera; mangrove; osmotic adjustment; protein (salt-sensitive); salt stress
Keywords Plus SALT-STRESS; WATER-DEFICIT; MOLECULAR RESPONSES; MEMBRANE-PROTEINS; TOLERANCE; ELECTROPHORESIS; CHLOROPLASTS; BARLEY; ROOTS
Abstract A significant decrease in the amount of a protein, whose migration in two-dimensional gel electrophoresis corresponds to an apparent molecular mass of 23 kDa and pI= 6.5, was observed in leaves of NaCl-treated Bruguiera parviflora (Roxb.) Wt. & Arn. ex Griff. seedlings. This particular salt-sensitive protein, designated as SSP-23, almost disappeared after 45 days of treatment in 400 mM NaCl as compared to untreated seedlings ( 0 mM NaCl) where the presence of the protein was significant. A polyclonal antibody raised against the 23-kDa protein was used to determine the subcellular localization of this protein in leaves by cross-reaction with proteins from isolated chloroplasts, mitochondria, peroxisomes and cytosol fractions on Western blots. SSP-23 was confirmed to be localized in the cytosol by immunoblotting. The disappearance of SSP-23 as a result of high NaCl treatment suggests that this protein is salt-sensitive and has a possible role in salt adaptation.
Author Address Natl Inst Plant Biodivers Conservat & Res, Bhubaneswar 751015, Orissa, India; Reg Plant Resource Ctr, Bhubaneswar 751015, Orissa, India; Univ Delhi, Sch Environm Studies, Ctr Environm Management Degraded Ecosyst, Delhi 110007, India; All India Inst Med Sci, Electron Microscopy Facil, New Delhi 110019, India
Reprint Address Das, AB (reprint author), Natl Inst Plant Biodivers Conservat & Res, Bhubaneswar 751015, Orissa, India.
E-mail Address a_b_das@hotmail.com
ResearcherID Number PARIDA, ASISH KUMAR/C-4771-2009
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Cited Reference Count 27
Times Cited 10
Total Times Cited Count (WoS, BCI, and CSCD) 14
Publisher SPRINGER
Publisher City NEW YORK
Publisher Address 233 SPRING STREET, NEW YORK, NY 10013 USA
ISSN 0032-0935
29-Character Source Abbreviation PLANTA
ISO Source Abbreviation Planta
Publication Date APR
Year Published 2005
Volume 221
Issue 1
Beginning Page 135
Ending Page 140
Digital Object Identifier (DOI) 10.1007/s00425-004-1415-2
Page Count 6
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number 919TX
Unique Article Identifier WOS:000228641600016
Plants associated with this reference

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