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Publication Type J
Authors Liu, SW; Xu, M; Tuo, L; Li, XJ; Hu, L; Chen, L; Li, RF; Sun, CH
Author Full Name Liu, Shao-Wei; Xu, Min; Tuo, Li; Li, Xiao-Jun; Hu, Lin; Chen, Li; Li, Rong-Feng; Sun, Cheng-Hang
Title Phycicoccus endophyticus sp nov., an endophytic actinobacterium isolated from Bruguiera gymnorhiza
Source INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Language English
Document Type Article
Keywords Plus GEN. NOV.; EMENDED DESCRIPTION; DEOXYRIBONUCLEIC-ACID; FOREST SOIL; TETRASPHAERA; CHINA; CHROMATOGRAPHY; MEMBER
Abstract A novel endophytic actinobacterium, designated strain IP6SC6(T), was isolated from surface-sterilized bark of Bruguiera gymnorhiza collected from Zhanjiang Mangrove Forest National Nature Reserve in Guangdong, China. Cells of strain IP6SC6(T) were Gram-stain-positive, aerobic, non-spore-forming, non-motile rods. Strain IP6SC6(T) grew at 20-42 degrees C (optimum, 37 degrees C), at pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-8 % (w/v) NaCl (optimum, 0-2 %). Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diamino acid of the peptidoglycan, galactose and glucose as whole-cell sugars, and MK-8(H-4) as the predominant menaquinone. The major polar lipids were diphosphatidylglycerol, phosphatidylinositol and an unknown lipid. The major fatty acids were iso-C-15 : 0, anteiso-C-15: 0, anteiso-C-17 : 0 and iso-C-16 : 0. The G + C content of the genomic DNA was 72.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain IP6SC6(T) belonged to the genus Phycicoccus and shared the highest sequence similarity with Phycicoccus jejuensis NRRL B-24460(T) (96.97 %). On the basis of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain IP6SC6(T) represents a novel species of the genus Phycicoccus, for which the name Phycicoccus endophyticus sp. nov. is proposed. The type strain is IP6SC6(T) (5DSM 100020(T)=CGMCC 4.7300(T)).
Author Address [Liu, Shao-Wei; Tuo, Li; Li, Xiao-Jun; Sun, Cheng-Hang] Chinese Acad Med Sci, Inst Med Biotechnol, Beijing 100050, Peoples R China; [Liu, Shao-Wei; Tuo, Li; Li, Xiao-Jun; Sun, Cheng-Hang] Peking Union Med Coll, Beijing 100050, Peoples R China; [Xu, Min] Guilin Med Univ, Fac Basic Med Sci, Guilin 541004, Peoples R China; [Li, Xiao-Jun] Hebei North Univ, Coll Lab Med Sci, Zhangjiakou 075000, Peoples R China; [Hu, Lin; Chen, Li] Chinese Acad Sci, Inst Zool, Beijing 100101, Peoples R China; [Li, Rong-Feng] Johns Hopkins Univ, Dept Chem, Charles & 34Th St, Baltimore, MD 21218 USA
Reprint Address Sun, CH (reprint author), Chinese Acad Med Sci, Inst Med Biotechnol, Beijing 100050, Peoples R China.; Sun, CH (reprint author), Peking Union Med Coll, Beijing 100050, Peoples R China.
E-mail Address chenghangsun@hotmail.com
Funding Agency and Grant Number National Natural Sciences Foundation of China (NSFC) [81172963, 81373308]; National Science and Technology Major Project from the Ministry of Science and Technology of China [2012ZX09301-002-001-018]
Funding Text We are grateful to Dr J.-N. Liang at the Institute of Microbiology, Chinese Academy of Sciences, for her assistance with transmission electron microscopy. This research was supported by the National Natural Sciences Foundation of China (NSFC, Grant nos. 81172963 and 81373308), and the National Science and Technology Major Project (Grant no. 2012ZX09301-002-001-018) from the Ministry of Science and Technology of China.
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Publisher SOC GENERAL MICROBIOLOGY
Publisher City READING
Publisher Address MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND
ISSN 1466-5026
29-Character Source Abbreviation INT J SYST EVOL MICR
ISO Source Abbreviation Int. J. Syst. Evol. Microbiol.
Publication Date MAR
Year Published 2016
Volume 66
Part Number 3
Beginning Page 1105
Ending Page 1111
Digital Object Identifier (DOI) 10.1099/ijsem.0.000842
Page Count 7
Web of Science Category Microbiology
Subject Category Microbiology
Document Delivery Number DJ3JX
Unique Article Identifier WOS:000374102200001
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