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Publication Type J
Authors Ezawa, S; Tada, Y
Author Full Name Ezawa, Shota; Tada, Yuichi
Title Identification of salt tolerance genes from the mangrove plant Bruguiera gymnorhiza using Agrobacterium functional screening
Source PLANT SCIENCE
Language English
Document Type Article
Author Keywords Agrobacterium functional screening; Bruguiera gymnorhiza; cDNA expression library; Mangrove; Salt tolerance
Keywords Plus ARABIDOPSIS-THALIANA; TRANSCRIPTION FACTOR; STRESS TOLERANCE; MOLECULAR CHARACTERIZATION; OVER-EXPRESSION; OSMOTIC-STRESS; HIGH-SALINITY; PROTEIN GENE; ENTH DOMAIN; GYMNORRHIZA
Abstract To identify key genes in the regulation of salt tolerance in the mangrove plant Bruguiera gymnorhiza, cDNA expression libraries were constructed from salt-treated roots and leaves using the host organism Agrobacterium tumefaciens. Functional screening of the Agrobacterium libraries identified 44 putative salt tolerance genes in B.gymnorhiza. A cDNA clone which is homologous to an unknown cDNA from the mangrove plant K. candel and the cyc02 gene from Catharanthus roseus conferred the highest level of salt tolerance (450 mM NaCl) to A. tumefaciens, indicating that it plays a major role in the regulation of salt tolerance in mangrove plants. Several of the genes that we identified have not previously been implicated in plant salt tolerance. Transgenic Arabidopsis plants expressing Bg70 and cyc02 homologue exhibited increased tolerance to NaCl. These results demonstrate that Agrobacterium functional screening is an effective supplemental method to pre-screen genes involved in abiotic stress tolerance. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
Author Address [Tada, Yuichi] Tokyo Univ Technol, Sch Bion, Tokyo 1920982, Japan; [Ezawa, Shota] Tokyo Univ Technol, Grad Sch Bion, Tokyo 1920982, Japan
Reprint Address Tada, Y (reprint author), Tokyo Univ Technol, Sch Bion, 1404-1 Katakura, Tokyo 1920982, Japan.
E-mail Address tadayui@bs.teu.ac.jp
ORCID Number Tada, Yuichi/0000-0001-7093-1677
Funding Agency and Grant Number Ministry of Education, Culture, Sports, Science and Technology of Japan [(SA030051) 2003-2007]; KAKENHI [(20580008]
Funding Text We wish to thank Dr. Tsuyoshi Nakagawa (Shimane University) for providing us the destination vector pGWB2. This work was partially supported by the Ministry of Education, Culture, Sports, Science and Technology of Japan,
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Cited Reference Count 43
Times Cited 18
Total Times Cited Count (WoS, BCI, and CSCD) 20
Publisher ELSEVIER IRELAND LTD
Publisher City CLARE
Publisher Address ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND
ISSN 0168-9452
29-Character Source Abbreviation PLANT SCI
ISO Source Abbreviation Plant Sci.
Publication Date FEB
Year Published 2009
Volume 176
Issue 2
Beginning Page 272
Ending Page 278
Digital Object Identifier (DOI) 10.1016/j.plantsci.2008.11.005
Page Count 7
Web of Science Category Biochemistry & Molecular Biology; Plant Sciences
Subject Category Biochemistry & Molecular Biology; Plant Sciences
Document Delivery Number 396BG
Unique Article Identifier WOS:000262566600014
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