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Publication Type J
Authors Wong, YY; Ho, CL; Nguyen, PD; Teo, SS; Harikrishna, JA; Rahim, RA; Wong, MCVL
Author Full Name Wong, Yeen-Yee; Ho, Chai-Ling; Nguyen, Phuoc Dang; Teo, Swee-Sen; Harikrishna, Jennifer Ann; Rahim, Raha Abdul; Wong, Michael C. V. L.
Title Isolation of salinity tolerant genes from the mangrove plant, Bruguiera cylindrica by using suppression subtractive hybridization (SSH) and bacterial functional screening
Source AQUATIC BOTANY
Language English
Document Type Article
Author Keywords mangrove; bacterial functional screening; Bruguiera cylindrica; salinity tolerance; sequence analysis; suppression subtractive hybridization (SSH)
Keywords Plus SALT-STRESS; SUPEROXIDE-DISMUTASE; RESPONSIVE GENES; ARABIDOPSIS; GYMNORRHIZA; EXPRESSION; IDENTIFICATION; GIGANTEA; PROTEIN
Abstract In this study, we have identified and isolated 126 salinity tolerant cDNAs from the root of a mangrove plant, Bruguiera cylindrica (L.) Blume by using suppression subtractive hybridization (SSH) and bacterial functional screening. Sequencing of 51 subtracted cDNA clones that were differentially expressed in the root of B. cylindrica exposed to 20 parts per thousand (ppt) NaCl water revealed 10 tentative unique genes (TUGs) with putative functions in protein synthesis, storage and destination, metabolism, intracellular trafficking and other functions; and 9 unknown proteins. Meanwhile, the 75 cDNA sequences of B. cylindrica that conferred salinity tolerance to Escherichia coli consisted of 29 TUGs with putative functions in transportation, metabolism and other functions; and 33 with unknown functions. Both approaches yielded 42 unique sequencess that have not been reported else where to be stress related and might provide further understanding of adaptations of this plant to salinity stress. (c) 2006 Elsevier B.V. All rights reserved.
Author Address Univ Pertanian Malaysia, Fac Biotechnol & Biomol Sci, Serdang 43400, Selangor, Malaysia; MUST, Unit GL33, Petaling Jaya 47301, Selangor, Malaysia; Univ Malaysia Sabah, Biotechnol Res Inst, Kota Kinabalu 88999, Sabah, Malaysia
Reprint Address Ho, CL (reprint author), Univ Pertanian Malaysia, Fac Biotechnol & Biomol Sci, Serdang 43400, Selangor, Malaysia.
E-mail Address clho@putra.upm.edu.my
ResearcherID Number Harikrishna, Jennifer/B-2778-2010; Wong, Clemente Michael /M-8372-2013
ORCID Number Harikrishna, Jennifer/0000-0002-4687-709X;
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Cited Reference Count 32
Times Cited 19
Total Times Cited Count (WoS, BCI, and CSCD) 26
Publisher ELSEVIER SCIENCE BV
Publisher City AMSTERDAM
Publisher Address PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
ISSN 0304-3770
29-Character Source Abbreviation AQUAT BOT
ISO Source Abbreviation Aquat. Bot.
Publication Date FEB
Year Published 2007
Volume 86
Issue 2
Beginning Page 117
Ending Page 122
Digital Object Identifier (DOI) 10.1016/j.aquabot.2006.09.009
Page Count 6
Web of Science Category Plant Sciences; Marine & Freshwater Biology
Subject Category Plant Sciences; Marine & Freshwater Biology
Document Delivery Number 130TX
Unique Article Identifier WOS:000243823000003
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