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Publication Type J
Authors Ben Mansour, R., M. K. Wided, S. Cluzet, S. Krisab, T. Richard and R. Ksouri
Title LC-MS identification and preparative HPLC isolation of Frankenia pulverulenta phenolics with antioxidant and neuroprotective capacities in PC12 cell line
Source Pharmaceutical Biology
Author Keywords beta-Amyloid peptide LC-DADESI-MS fractionation MTT test ORAC in-vitro antioxidant oxidative stress mitochondrial dysfunction lipid-peroxidation mass-spectrometry induced toxicity ellagic acid assay fluorescein aggregation
Abstract Context: Frankenia pulverulenta L. (Frankeniaceae) is a medicinal species with carminative, analgesic and antiviral properties. However, phytochemical investigations, antioxidant and neuroprotective capacities of this plant remain unclear. Objective: This work assesses the phenolic composition of F. pulverulenta shoot and root and evaluates their antioxidant and neuroprotective capacities. Materials and methods: Successive fractionation of F. pulverulenta shoot and root using 6 solvents were used. Antioxidant capacity of these fractions was assessed through four in vitro tests (DPPH, ABTS, Fe-che-lating activity and ORAC). Phenolic identification, purification as well as neuroprotective activity of ethyl acetate (EtOAc) fraction and purified molecules were assessed. Results: Among the tested fractions, EtOAc shoot and root fractions possessed considerable phenolic contents (383 and 374mg GAE/g E, respectively) because of their important ORAC (821 and 1054mg of TE/g E), DPPH (586 and 750mg of TE/g) and ABTS (1453 and 1319mg of TE/g) results. Moreover, gallic acid, quercetin, quercetin galloyl glucoside, trigalloyl hexoside, procyanidin dimers and sulfated flavonoids were identified by LC-DAD-ESI-MS for the first time in this species. The relevant cytoprotective capacity (at 300 mu g/mL) against beta-amyloid peptide induced toxicity in PC12 cells of EtOAc fractions were corroborated with the chemical composition. In addition, purified molecules were tested for their ORAC and neuroprotective activity. Quercetin showed the best ORAC value (33.55 mmol TE/g polyphenols); nevertheless, procyanidin dimer exhibited an exceptionally efficient neuroprotective activity (100% of viability at 50 mu g/mL). Discussion and conclusions: These findings suggest that this halophyte is a promising source of antioxidant and neuroprotective molecules for pharmaceutical purposes.
Author Address [Ben Mansour, Rim; Wided, Megdiche Ksouri; Ksouri, Riadh] Ctr Biotechnol Technopk Borj Cedria CBBC, Lab Aromat & Med Plants, BP 901, Hammam Lif 2050, Tunisia. [Cluzet, Stephanie; Krisab, Stephanie; Richard, Tristan] Univ Bordeaux, ISW, GESVAB EA3675, Villenave Dornon, France. Ben Mansour, R (reprint author), Ctr Biotechnol Technopk Borj Cedria CBBC, Lab Aromat & Med Plants, BP 901, Hammam Lif 2050, Tunisia. rim_ben_mansour@hotmail.fr
ISSN 1388-0209
ISBN 1388-0209
29-Character Source Abbreviation Pharm. Biol.
Year Published 2017
Volume 55
Issue 1
Beginning Page 880-887
Digital Object Identifier (DOI) 10.1080/13880209.2016.1278452
Unique Article Identifier WOS:000395131900016

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