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Publication Type J
Authors Ragasa, CY; Espineli, DL; Shen, CC
Author Full Name Ragasa, Consolacion Y.; Espineli, Dinah L.; Shen, Chien-Chang
Title Cytotoxic Triterpene from Barringtonia asiatica
Source PHARMACEUTICAL CHEMISTRY JOURNAL
Language English
Document Type Article
Author Keywords Barringtonia asiatica; Lecythidaceae; betulinic acid; germanicol; MTT; cytotoxic activity
Keywords Plus BETULINIC ACID; CELLS; APOPTOSIS; CANCER; ACTIVATION; SAPONINS; SQUALENE; AGENT; SEEDS
Abstract Triterpenes isolated from the dichloromethane extract of Barringtonia asiatica, namely, a mixture of betulinic acid (1) and 22-O-tigloylcamelliagenin A (2) in a 1 : 2 ratio and a mixture of 3 beta-olean-18-en-3-yl palmitate (7), 3 beta-urs-12-en-3-yl palmitate (8) and 3 beta-olean-12-en-3-yl palmitate (9) in a 4 : 1 : 2 ratio (isolated from the bark), as well as germanicol caffeoyl ester (3), germanicol trans-coumaroyl ester (4), germanicol cis-coumaroyl ester (5) and germanicol (6) (from the leaves), and a phenolic compound, verimol K (10) from the flowers, were assessed for cytotoxicity against a human cancer cell line, colon carcinoma (HCT 116), using the MTT assay. The mixture of 1 and 2 exhibited IC50 =8.0 mu g center dot mL(-1) against this cell line, while 6 exhibited an IC50 value of 29.6 mu g center dot mL(-1). The other compounds tested (3 - 5, 7 - 10) were inactive against the HCT 116 cell line. The mixture of 1 and 2 and compound 6 were further tested for cytotoxicity against the non-small cell lung adenocarcinoma (A549) cell line. The mixture of 1 and 2 and compound 6 exhibited IC50 values of 6.0 and 35.6 mu g center dot mL(-1), respectively. The cytotoxicity for the mixture of 1 and 2 may be attributed to betulinic acid (1), a known cytotoxic compound.
Author Address [Ragasa, Consolacion Y.; Espineli, Dinah L.] De La Salle Univ, Dept Chem, Manila 1004, Philippines; [Ragasa, Consolacion Y.; Espineli, Dinah L.] De La Salle Univ, Ctr Nat Sci & Ecol Res, Manila 1004, Philippines; [Shen, Chien-Chang] Natl Res Inst Chinese Med, Taipei 112, Taiwan
Reprint Address Ragasa, CY (reprint author), De La Salle Univ, Dept Chem, 2401 Taft Ave, Manila 1004, Philippines.
E-mail Address consolacion.ragasa@dlsu.edu.ph
Funding Agency and Grant Number Centennial Fund of De La Salle University - Manila
Funding Text A research grant from the Centennial Fund of De La Salle University - Manila is gratefully acknowledged. The MTT assay was conducted at the Institute of Biology, University of the Philippines, Diliman, Quezon City.
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Cited Reference Count 29
Publisher SPRINGER
Publisher City NEW YORK
Publisher Address 233 SPRING ST, NEW YORK, NY 10013 USA
ISSN 0091-150X
29-Character Source Abbreviation PHARM CHEM J+
ISO Source Abbreviation Pharm. Chem. J.
Publication Date NOV
Year Published 2014
Volume 48
Issue 8
Beginning Page 529
Ending Page 533
Digital Object Identifier (DOI) 10.1007/s11094-014-1144-1
Page Count 5
Web of Science Category Chemistry, Medicinal; Pharmacology & Pharmacy
Subject Category Pharmacology & Pharmacy
Document Delivery Number AX4JQ
Unique Article Identifier WOS:000346899500008
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