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| Publication Type | J |
| Authors | Sporna-Kucab, A; Ignatova, S; Garrard, I; Wybraniec, S |
| Author Full Name | Sporna-Kucab, Aneta; Ignatova, Svetlana; Garrard, Ian; Wybraniec, Slawomir |
| Title | Versatile solvent systems for the separation of betalains from processed Beta vulgaris L. juice using counter-current chromatography |
| Source | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES |
| Language | English |
| Document Type | Article |
| Author Keywords | Betanin; Betalains; Betacyanins; Counter-current chromatography; Beta vulgaris L |
| Keywords Plus | DECARBOXYLATED BETACYANINS; BOUGAINVILLEA-GLABRA; OXIDATION |
| Abstract | Two mixtures of decarboxylated and dehydrogenated betacyanins from processed red beet roots (Beta vulgaris L) juice were fractionated by high performance counter-current chromatography (HPCCC) producing a range of isolated components. Mixture 1 contained mainly betacyanins, 14,15-dehydro-betanin (neobetanin) and their decarboxylated derivatives while mixture 2 consisted of decarboxy- and dehydro-betacyanins. The products of mixture 1 arose during thermal degradation of betanin/isobetanin in mild conditions while the dehydro-betacyanins of mixture 2 appeared after longer heating of the juice from B. vulgaris L. Two solvent systems were found to be effective for the HPCCC. A highly polar, high salt concentration system of 1-PrOH-ACN-(NH4)(2)SO4 (satd. soln)-water (v/v/v/v, 1:0.:1.2:1) (tail-to-head mode) enabled the purification of 2-decarboxy-betanin/-isobetanin, 2,17-bidecarboxy-betanin/-isobetanin and neobetanin (all from mixture 1) plus 17-decarboxy-neobetanin, 2,15,17-tridecarboxy-2,3-dehydro-neobetanin, 2-decarboxy-neobetanin and 2,15,17-tridecarboxy-neobetanin (from mixture 2). The other solvent system included heptafluorobutyric acid (HFBA) as ion-pair reagent and consisted of tert-butyl methyl ether (TBME)-1-BuOH-ACN-water (acidified with 0.7% HFBA) (2:2:1:5, v/v/v/v) (head-to-tail mode). This system enabled the HPCCC purification of 2,17-bidecarboxy-betanin/-isobetanin and neobetanin (from mixture 1) plus 2,15,17-tridecarboxy-2,3-dehydro-neobetanin, 2,17-bidecarboxy-2,3-dehydro-neobetanin and 2,15,17-tridecarboxy-neobetanin (mixture 2). The results of this research are crucial in finding effective isolation methods of betacyanins and their derivatives which are meaningful compounds due their colorant properties and potential health benefits regarding antioxidant and cancer prevention. The pigments were detected by LC-DAD and LC-MS/MS techniques. (C) 2013 Elsevier B.V. All rights reserved. |
| Author Address | [Sporna-Kucab, Aneta; Wybraniec, Slawomir] Cracow Univ Technol, Dept Analyt Chem, Inst C1, Fac Chem Engn & Technol, PL-31155 Krakow, Poland; [Ignatova, Svetlana; Garrard, Ian] Brunel Univ, Brunel Inst Bioengn, Uxbridge UB8 3PH, Middx, England |
| Reprint Address | Sporna-Kucab, A (reprint author), Cracow Univ Technol, Dept Analyt Chem, Inst C1, Fac Chem Engn & Technol, Ul Warszawska 24, PL-31155 Krakow, Poland. |
| E-mail Address | anetasporna@chemia.pk.edu.pl |
| Funding Agency and Grant Number | European Union through the European Social Fund [UDA-POKL.04.01.01-00-029/10-00]; UK Higher Education Infrastructure Fund [HEIF4] |
| Funding Text | The research was supported by the European Union through the European Social Fund within |
| Cited References | Berthod A., 2002, COUNTERCURRENT CHROM; Costa FD, 2010, J SEP SCI, V33, P336, DOI 10.1002/jssc.200900632; Dagenhardt A., 2001, J LIQ CHROMATOGR R T, V24, P1745; Fahey JW, 2003, J CHROMATOGR A, V996, P85, DOI 10.1016/S0021-9673(03)00607-1; Fisher D, 2005, J LIQ CHROMATOGR R T, V28, P1913, DOI 10.1081/JLC-200063563; Herbach KM, 2004, EUR FOOD RES TECHNOL, V219, P377, DOI 10.1007/s00217-004-0948-8; Herbach K.M., 2006, J FOOD SCI, V71, P41, DOI DOI 10.1111/J.1750-3841.2006.00022.X; Ito Y, 2005, J CHROMATOGR A, V1065, P145, DOI 10.1016/j.chroma.2004.12.044; Ito Y., 1996, CHEM ANAL, V132; Jerz G, 2008, J CHROMATOGR A, V1190, P63, DOI 10.1016/j.chroma.2008.02.093; Jerz G, 2010, J CHROMATOGR A, V1217, P4544, DOI 10.1016/j.chroma.2010.04.061; Stintzing FC, 2007, TRENDS FOOD SCI TECH, V18, P514, DOI 10.1016/j.tifs.2007.04.012; Strack D, 1993, METHODS PLANT BIOCH, V8, P421; Winterhalter P., 2007, TRENDS FOOD SCI TECH, V18, P508; Wybraniec S, 2006, TETRAHEDRON LETT, V47, P1725, DOI 10.1016/j.tetlet.2006.01.046; Wybraniec S, 2005, J AGR FOOD CHEM, V53, P3483, DOI 10.1021/jf048088d; Wybraniec S, 2004, J CHROMATOGR A, V1029, P97, DOI 10.1016/j.chroma.2003.12.055; Wybraniec S, 2007, ANAL BIOANAL CHEM, V389, P1611, DOI 10.1007/s00216-007-1550-2; Wybraniec S, 2013, J AGR FOOD CHEM, V61, P6465, DOI 10.1021/jf400818s; Wybraniec S, 2011, J AGR FOOD CHEM, V59, P9612, DOI 10.1021/jf2020107; Wybraniec S, 2010, J CHROMATOGR B, V878, P538, DOI 10.1016/j.jchromb.2009.12.029; Wybraniec S, 2009, J CHROMATOGR A, V1216, P6890, DOI 10.1016/j.chroma.2009.08.035 |
| Cited Reference Count | 22 |
| Times Cited | 8 |
| Total Times Cited Count (WoS, BCI, and CSCD) | 8 |
| Publisher | ELSEVIER SCIENCE BV |
| Publisher City | AMSTERDAM |
| Publisher Address | PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
| ISSN | 1570-0232 |
| 29-Character Source Abbreviation | J CHROMATOGR B |
| ISO Source Abbreviation | J. Chromatogr. B |
| Publication Date | DEC 15 |
| Year Published | 2013 |
| Volume | 941 |
| Beginning Page | 54 |
| Ending Page | 61 |
| Digital Object Identifier (DOI) | 10.1016/j.jchromb.2013.10.001 |
| Page Count | 8 |
| Web of Science Category | Biochemical Research Methods; Chemistry, Analytical |
| Subject Category | Biochemistry & Molecular Biology; Chemistry |
| Document Delivery Number | 257ET |
| Unique Article Identifier | WOS:000327367100009 |
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