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Publication Type J
Authors Sporna-Kucab, A; Ignatova, S; Garrard, I; Wybraniec, S
Author Full Name Sporna-Kucab, Aneta; Ignatova, Svetlana; Garrard, Ian; Wybraniec, Slawomir
Title Versatile solvent systems for the separation of betalains from processed Beta vulgaris L. juice using counter-current chromatography
Source JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Language English
Document Type Article
Author Keywords Betanin; Betalains; Betacyanins; Counter-current chromatography; Beta vulgaris L
Keywords Plus DECARBOXYLATED BETACYANINS; BOUGAINVILLEA-GLABRA; OXIDATION
Abstract Two mixtures of decarboxylated and dehydrogenated betacyanins from processed red beet roots (Beta vulgaris L) juice were fractionated by high performance counter-current chromatography (HPCCC) producing a range of isolated components. Mixture 1 contained mainly betacyanins, 14,15-dehydro-betanin (neobetanin) and their decarboxylated derivatives while mixture 2 consisted of decarboxy- and dehydro-betacyanins. The products of mixture 1 arose during thermal degradation of betanin/isobetanin in mild conditions while the dehydro-betacyanins of mixture 2 appeared after longer heating of the juice from B. vulgaris L. Two solvent systems were found to be effective for the HPCCC. A highly polar, high salt concentration system of 1-PrOH-ACN-(NH4)(2)SO4 (satd. soln)-water (v/v/v/v, 1:0.:1.2:1) (tail-to-head mode) enabled the purification of 2-decarboxy-betanin/-isobetanin, 2,17-bidecarboxy-betanin/-isobetanin and neobetanin (all from mixture 1) plus 17-decarboxy-neobetanin, 2,15,17-tridecarboxy-2,3-dehydro-neobetanin, 2-decarboxy-neobetanin and 2,15,17-tridecarboxy-neobetanin (from mixture 2). The other solvent system included heptafluorobutyric acid (HFBA) as ion-pair reagent and consisted of tert-butyl methyl ether (TBME)-1-BuOH-ACN-water (acidified with 0.7% HFBA) (2:2:1:5, v/v/v/v) (head-to-tail mode). This system enabled the HPCCC purification of 2,17-bidecarboxy-betanin/-isobetanin and neobetanin (from mixture 1) plus 2,15,17-tridecarboxy-2,3-dehydro-neobetanin, 2,17-bidecarboxy-2,3-dehydro-neobetanin and 2,15,17-tridecarboxy-neobetanin (mixture 2). The results of this research are crucial in finding effective isolation methods of betacyanins and their derivatives which are meaningful compounds due their colorant properties and potential health benefits regarding antioxidant and cancer prevention. The pigments were detected by LC-DAD and LC-MS/MS techniques. (C) 2013 Elsevier B.V. All rights reserved.
Author Address [Sporna-Kucab, Aneta; Wybraniec, Slawomir] Cracow Univ Technol, Dept Analyt Chem, Inst C1, Fac Chem Engn & Technol, PL-31155 Krakow, Poland; [Ignatova, Svetlana; Garrard, Ian] Brunel Univ, Brunel Inst Bioengn, Uxbridge UB8 3PH, Middx, England
Reprint Address Sporna-Kucab, A (reprint author), Cracow Univ Technol, Dept Analyt Chem, Inst C1, Fac Chem Engn & Technol, Ul Warszawska 24, PL-31155 Krakow, Poland.
E-mail Address anetasporna@chemia.pk.edu.pl
Funding Agency and Grant Number European Union through the European Social Fund [UDA-POKL.04.01.01-00-029/10-00]; UK Higher Education Infrastructure Fund [HEIF4]
Funding Text The research was supported by the European Union through the European Social Fund within
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Cited Reference Count 22
Times Cited 8
Total Times Cited Count (WoS, BCI, and CSCD) 8
Publisher ELSEVIER SCIENCE BV
Publisher City AMSTERDAM
Publisher Address PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
ISSN 1570-0232
29-Character Source Abbreviation J CHROMATOGR B
ISO Source Abbreviation J. Chromatogr. B
Publication Date DEC 15
Year Published 2013
Volume 941
Beginning Page 54
Ending Page 61
Digital Object Identifier (DOI) 10.1016/j.jchromb.2013.10.001
Page Count 8
Web of Science Category Biochemical Research Methods; Chemistry, Analytical
Subject Category Biochemistry & Molecular Biology; Chemistry
Document Delivery Number 257ET
Unique Article Identifier WOS:000327367100009
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