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Publication Type J
Authors Jithesh, M. N., S. R. Prashanth, K. R. Sivaprakash and A. Parida
Title Monitoring expression profiles of antioxidant genes to salinity, iron, oxidative, light and hyperosmotic stresses in the highly salt tolerant grey mangrove, Avicennia marina (Forsk.) Vierh. by mRNA analysis
Source Plant Cell Reports
Author Keywords Avicennia marina catalase Cu-ZnSOD ferritin ROS cytosolic ascorbate peroxidase nicotiana-plumbaginifolia l superoxide-dismutase ferritin synthesis transgenic plants oxygen radicals salicylic-acid water-deficit metabolism leaves
Abstract Plant photosynthesis results in the production of molecular oxygen. An inevitable consequence of this normal process is the production of reactive oxygen species (ROS) by the transfer of electrons to molecular oxygen. Plants are adequately protected by the presence of multiple antioxidative enzymes in different organelles of the plant such as chloroplasts, cytosol, mitochondria and peroxisomes. Under high light and CO2 limiting conditions caused by environmental stress like salinity, these antioxidative enzymes play an important role in scavenging toxic radicals. To investigate the functions of antioxidative enzymes in a mangrove plant, we isolated three cDNAs encoding cytosolic Cu-Zn SOD (Sod1), catalase (Cat1) and ferritin (Fer1) from Avicennia marina cDNA library. Sod1, Cat1 and Fer1 cDNA encoded full-length proteins with 152, 492 and 261 amino acids respectively. We studied the expression of these antioxidant genes in response to salt, iron, hydrogen peroxide, mannitol and light stress by mRNA expression analysis. Cat1, Fer1 showed short-term induction while Sod1 transcript was found to be unaltered in response to NaCl stress. A decrease in mRNA levels was observed for Sod1, Cat1 while Fer1 mRNA levels remained unaltered with osmotic stress treatment. Sod1, Cat1 and Fer1 mRNA levels were induced by iron, light stress and by direct H2O2 stress treatment, thus confirming their role in oxidative stress response.
Author Address MS Swaminathan Res Fdn, Taramani Inst Area, Madras 600113, Tamil Nadu, India. Parida, A (reprint author), MS Swaminathan Res Fdn, Taramani Inst Area, 3 Cross St, Madras 600113, Tamil Nadu, India. ajay@mssrf.res.in
ISSN 0721-7714
ISBN 0721-7714
29-Character Source Abbreviation Plant Cell Reports
Publication Date Aug
Year Published 2006
Volume 25
Issue 8
Beginning Page 865-876
Digital Object Identifier (DOI) 10.1007/s00299-006-0127-4
Unique Article Identifier WOS:000239087900015
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