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Publication Type J
Authors Jyothi-Prakash, P. A., B. Mohanty, E. Wijaya, T. M. Lim, Q. S. Lin, C. S. Loh and P. P. Kumar
Title Identification of salt gland-associated genes and characterization of a dehydrin from the salt secretor mangrove Avicennia officinalis
Source Bmc Plant Biology
Author Keywords Avicennia officinalis Salinity Dehydrin Subtractive hybridization Leaf salt glands Drought stress suppression subtractive hybridization marina forssk vierh expression analysis aegiceras-corniculatum arabidopsis-thaliana salinity tolerance plant dehydrins abscisic-acid stress proteins
Abstract Background: Salt stress is a major challenge for growth and development of plants. The mangrove tree Avicennia officinalis has evolved salt tolerance mechanisms such as salt secretion through specialized glands on its leaves. Although a number of structural studies on salt glands have been done, the molecular mechanism of salt secretion is not clearly understood. Also, studies to identify salt gland-specific genes in mangroves have been scarce. Results: By subtractive hybridization (SH) of cDNA from salt gland-rich cell layers (tester) with mesophyll tissues as the driver, several Expressed Sequence Tags (ESTs) were identified. The major classes of ESTs identified include those known to be involved in regulating metabolic processes (37%), stress response (17%), transcription (17%), signal transduction (17%) and transport functions (12%). A visual interactive map generated based on predicted functional gene interactions of the identified ESTs suggested altered activities of hydrolase, transmembrane transport and kinases. Quantitative Real-Time PCR (qRT-PCR) was carried out to validate the expression specificity of the ESTs identified by SH. A Dehydrin gene was chosen for further experimental analysis, because it is significantly highly expressed in salt gland cells, and dehydrins are known to be involved in stress remediation in other plants. Full-length Avicennia officinalis Dehydrin1 (AoDHN1) cDNA was obtained by Rapid Amplification of cDNA Ends. Phylogenetic analysis and further characterization of this gene suggested that AoDHN1 belongs to group II Late Embryogenesis Abundant proteins. qRT-PCR analysis of Avicennia showed up-regulation of AoDHN1 in response to salt and drought treatments. Furthermore, some functional insights were obtained by growing E. coli cells expressing AoDHN1. Growth of E. coli cells expressing AoDHN1 was significantly higher than that of the control cells without AoDHN1 under salinity and drought stresses, suggesting that the mangrove dehydrin protein helps to mitigate the abiotic stresses. Conclusions: Thirty-four ESTs were identified to be enriched in salt gland-rich tissues of A. officinalis leaves. qRT-PCR analysis showed that 10 of these were specifically enriched in the salt gland-rich tissues. Our data suggest that one of the selected genes, namely, AoDHN1 plays an important role to mitigate salt and drought stress responses.
Author Address [Jyothi-Prakash, Pavithra A.; Lim, Tit-Meng; Lin, Qingsong; Loh, Chiang-Shiong; Kumar, Prakash P.] Natl Univ Singapore, Dept Biol Sci, Singapore 117548, Singapore. [Jyothi-Prakash, Pavithra A.; Loh, Chiang-Shiong] Natl Univ Singapore, NUS Environm Res Inst, Singapore 117548, Singapore. [Mohanty, Bijayalaxmi] Natl Univ Singapore, Dept Biomol & Chem Engn, Singapore 117548, Singapore. [Wijaya, Edward] Osaka Univ, IFReC, Suita, Osaka 5650871, Japan. [Kumar, Prakash P.] Natl Univ Singapore, Temasek Life Sci Lab, Singapore 117548, Singapore. Kumar, PP (reprint author), Natl Univ Singapore, Dept Biol Sci, 14 Sci Dr 4, Singapore 117548, Singapore. dbskumar@nus.edu.sg
ISSN 1471-2229
ISBN 1471-2229
29-Character Source Abbreviation BMC Plant Biol.
Publication Date Nov
Year Published 2014
Volume 14
Digital Object Identifier (DOI) 10.1186/s12870-014-0291-6
Unique Article Identifier WOS:000346927600002
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