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Publication Type J
Authors Wang, L. X., D. Z. Pan, X. J. Lv, C. L. Cheng, J. Li, W. Y. Liang, J. H. Xing and W. Chen
Title A multilevel investigation to discover why Kandelia candel thrives in high salinity
Source Plant Cell and Environment
Author Keywords DNA damage repair free amino acids mangrove tree phenylpropanoids salt tolerance gamma-aminobutyric-acid salt tolerance mangrove plant proteomic analysis glutamate-decarboxylase bruguiera-gymnorrhiza antioxidant activity dna-repair stress transcriptome
Abstract The halophilic mangrove species Kandelia candel is an excellent model for understanding why halophytes thrive in high salinity. Preliminary transcriptomic analyses revealed that genes involved in diverse functions, such as in phenylpropanoid and amino acid metabolisms, and those in DNA replication and damage repair were highly responsive to salt stress. Proteomic analyses revealed that the proteins involved in light reaction of photosynthesis, amino acid and carbohydrate metabolisms, secondary metabolite biosynthesis and posttranslational modification showed increased levels in response to salt stress. The metabolisms of phenylpropanoids and amino acids under salt stress were systematically examined based on the preliminary omics analyses. The activities of phenylpropanoid biosynthetic enzymes and the contents of phenols, flavonoids, anthocyanins and lignins were significantly increased under salt stress. In the free amino acid pool, glutamate was the most abundant. Together with -aminobutyric acid, glutamate levels further increased, while proline levels remained unchanged in response to salt stress. These findings point to the potential importance of phenylpropanoids and free amino acids in salt tolerance of K.candel that have been observed, but not systemically investigated at the levels of gene expression, enzyme activity and metabolite accumulation in glycophytes and non-tree halophytes. Little is known about salt-tolerance mechanisms in tree halophytes. In this study, systematic investigations at the levels of gene expression, enzyme activity and metabolite concentration based on preliminary RNA-Seq and proteomic analyses were conducted in Kandelia candel. The salt-tolerant characteristics discovered include the accumulation of phenylpropanoids and free amino acids, especially glutamate and -aminobutyric acid, but not proline.
Author Address [Wang, Lingxia; Pan, Dezhuo; Lv, Xiaojie; Li, Jian; Xing, Jianhong; Chen, Wei] Fujian Agr & Forestry Univ, Sch Life Sci, Fuzhou 350002, Peoples R China. [Wang, Lingxia; Liang, Wenyu] Ningxia Univ, Sch Life Sci, Yinchuan 750021, Peoples R China. [Cheng, Chi-Lien] Univ Iowa, Dept Biol, 210 Biol Bldg, Iowa City, IA 52242 USA. [Chen, Wei] Fujian Agr & Forestry Univ, Fujian Prov Key Lab Crop Mol & Cell Biol, Fuzhou 350002, Peoples R China. Chen, W (reprint author), Fujian Agr & Forestry Univ, Sch Life Sci, Fuzhou 350002, Peoples R China.; Chen, W (reprint author), Fujian Agr & Forestry Univ, Fujian Prov Key Lab Crop Mol & Cell Biol, Fuzhou 350002, Peoples R China. weichen909@163.com
ISSN 0140-7791
ISBN 0140-7791
29-Character Source Abbreviation Plant Cell Environ.
Publication Date Nov
Year Published 2016
Volume 39
Issue 11
Beginning Page 2486-2497
Digital Object Identifier (DOI) 10.1111/pce.12804
Unique Article Identifier WOS:000385842400012
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