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Publication Type J
Authors Aouissat, M., M. Belkhoja, K. Hcini, D. J. Walker and E. Correal
Title Estimation of nuclear DNA content in Algerian populations of Atriplex halimus and Atriplex canescens by flow cytometry
Source Anales de Biologia
Abstract Atriplex halimus is a perennial native shrub of the Mediterranean Basin with an excellent tolerance to drought and salinity, also, Atriplex canescens, four-wing saltbush, is adapted to desert climates. The aim of this work was to estimate nuclear DNA content in Algerian populations of A. halimus and A. canescens by flow cytometry, an efficient method to estimate ploidy levels and to determine nuclear DNA content in plants. Flow cytomertry is a technique which permits rapid estimation of nuclear DNA content. With respect to nuclear DNA, the 2C DNA content of population Cala Tarida (Spain) was estimated to be 2.46pg. There was no significant difference among the populations of A. halimus, whose 2C DNA content ranged from 4.91 to 5.04pg. However, the 2C DNA content of A. canescens ranged from 3.14 to 3.27.
Author Address Hcini, Kheiria; Fac Sci Tunis, Dept Sci Biol, El Manar II Tunis 2092, Tunisia
ISSN 1138-3399
ISBN 1138-3399
Year Published 2009
Volume 31
Beginning Page 15-18
Unique Article Identifier BCI:BCI201100551526
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