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Publication Type J
Authors Sun, X., P. Jia, J. Zhang and H. Pan
Title Cloning and characterization of a salt responsive gene AcPsbQ1 from Atriplex canescens
Source Yichuan
Abstract PsbQ is an extrinsic subunit of the photosystem II in eukaryotic photosynthetic organisms. Numerous studies have demonstrated that PsbQ can stabilize the inorganic cofactors and enhance the oxygen release in PSII. The decrease of photosynthesis rate under salinity condition is normally attributed to the high concentration of injurious ions, such as Na+ and C1, which accumulate in the chloroplast and damage thylakoid membrane under salinity stress. In this study, AcPsbQl was isolated from a halophyte Atriplex canescens cDNA library. The AcPsbQl contains an open reading frame of 699 bp encoding a 233 amino acid protein. In order to investigate its function, AcPsbQl was cloned and transformed into Saccharomyces cerevisiae INVScl. The heterologous expression of AcPsbQl in transgenic yeast significantly helped to increase the adapting and recovery ability of yeast cells under the salt and drought. Quantitative real-time PCR assay was performed to reveal the expression pattern of AcPsbQl under different abiotic stresses. On exposure to NaCl stress, the transcript level of AcPsbQl was significantly enhanced. AcPsbQl expression level was also up-regulated under drought stress. These results indicated that AcPsbQl might involve in the response to salt stress in A. canescens.
ISSN 0253-9772
ISBN 0253-9772
Publication Date Jan
Year Published 2015
Volume 37
Issue 1
Beginning Page 84-90
Digital Object Identifier (DOI) 10.16288/j.yczz.2015.01.012
Unique Article Identifier BCI:BCI201600720023
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