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Publication Type J
Authors Zhao, Q., J. W. Suo, S. X. Chen, Y. D. Jin, X. L. Ma, Z. P. Yin, Y. H. Zhang, T. Wang, J. Luo, W. H. Jin, X. Zhang, Z. Q. Zhou and S. J. Dai
Title Na2CO3-responsive mechanisms in halophyte Puccinellia tenuiflora roots revealed by physiological and proteomic analyses
Source Scientific Reports
Author Keywords stress-responsive proteins expressed sequence tags oryza-sativa l. salt-stress gene-expression environmental-stresses salinity tolerance over-expression binding protein seedling roots
Abstract Soil alkalization severely affects crop growth and agricultural productivity. Alkali salts impose ionic, osmotic, and high pH stresses on plants. The alkali tolerance molecular mechanism in roots from halophyte Puccinellia tenuiflora is still unclear. Here, the changes associated with Na2CO3 tolerance in P. tenuiflora roots were assessed using physiological and iTRAQ-based quantitative proteomic analyses. We set up the first protein dataset in P. tenuiflora roots containing 2,671 non-redundant proteins. Our results showed that Na2CO3 slightly inhibited root growth, caused ROS accumulation, cell membrane damage, and ion imbalance, as well as reduction of transport and protein synthesis/turnover. The Na2CO3-responsive patterns of 72 proteins highlighted specific signaling and metabolic pathways in roots. Ca2+ signaling was activated to transmit alkali stress signals as inferred by the accumulation of calcium-binding proteins. Additionally, the activities of peroxidase and glutathione peroxidase, and the peroxiredoxin abundance were increased for ROS scavenging. Furthermore, ion toxicity was relieved through Na+ influx restriction and compartmentalization, and osmotic homeostasis reestablishment due to glycine betaine accumulation. Importantly, two transcription factors were increased for regulating specific alkali-responsive gene expression. Carbohydrate metabolism-related enzymes were increased for providing energy and carbon skeletons for cellular metabolism. All these provide new insights into alkali-tolerant mechanisms in roots.
Author Address [Zhao, Qi; Zhang, Yuhong; Zhou, Zhiqiang; Dai, Shaojun] Northeast Forestry Univ, Key Lab Forest Plant Ecol, Minist Educ, Harbin 150040, Peoples R China. [Zhao, Qi; Suo, Jinwei; Jin, Yudan; Ma, Xiaolin; Dai, Shaojun] Northeast Forestry Univ, Alkali Soil Nat Environm Sci Ctr, Key Lab Salinealkali Vegetat Ecol Restorat Oil Fi, Minist Educ, Harbin 150040, Peoples R China. [Chen, Sixue] Univ Florida, Interdisciplinary Ctr Biotechnol Res, Genet Inst, Dept Biol,Plant Mol & Cellular Biol Program, Gainesville, FL 32610 USA. [Wang, Tai; Zhang, Xia] Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China. [Luo, Ji; Jin, Wenhai] AB Sciex Asia Pacific Applicat Support Ctr, Shanghai 200233, Peoples R China. Zhou, ZQ; Dai, SJ (reprint author), Northeast Forestry Univ, Key Lab Forest Plant Ecol, Minist Educ, Harbin 150040, Peoples R China.; Dai, SJ (reprint author), Northeast Forestry Univ, Alkali Soil Nat Environm Sci Ctr, Key Lab Salinealkali Vegetat Ecol Restorat Oil Fi, Minist Educ, Harbin 150040, Peoples R China. zhiqiangzhou@hotmail.com; daishaojun@hotmail.com
ISSN 2045-2322
ISBN 2045-2322
29-Character Source Abbreviation Sci Rep
Publication Date Sep
Year Published 2016
Volume 6
Digital Object Identifier (DOI) 10.1038/srep32717
Unique Article Identifier WOS:000382467100001
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