Publication Type | J |
Authors | Kamal, Z; Ullah, F; Ayaz, M; Sadiq, A; Ahmad, S; Zeb, A; Hussain, A; Imran, M |
Author Full Name | Kamal, Zul; Ullah, Farhat; Ayaz, Muhammad; Sadiq, Abdul; Ahmad, Sajjad; Zeb, Anwar; Hussain, Abid; Imran, Muhammad |
Title | Anticholinesterse and antioxidant investigations of crude extracts, subsequent fractions, saponins and flavonoids of atriplex laciniata L.: potential effectiveness in Alzheimer's and other neurological disorders |
Source | BIOLOGICAL RESEARCH |
Language | English |
Document Type | Article |
Author Keywords | Phenolic & flavonoid contents; DPPH; ABTS; H2O2; Gallic acid; IC50 and anticholinesterase |
Keywords Plus | RANDOMIZED CONTROLLED-TRIAL; TOTAL PHENOLIC CONTENTS; POLYGONUM-HYDROPIPER L; TRADITIONAL MEDICINE; METHANOLIC EXTRACT; DISEASE; ACETYLCHOLINESTERASE; PLANTS; CHINESE; PHYTOCHEMICALS |
Abstract | Background: Atriplex laciniata L. was investigated for phenolic, flavonoid contents, antioxidant, anticholinesterase activities, in an attempt to explore its effectiveness in Alzheimer's and other neurological disorders. Plant crude methanolic extract (Al.MeF), subsequent fractions; n-hexane (Al.HxF), chloroform (Al.CfF), ethyl acetate (Al.EaF), aqueous (Al.WtF), Saponins (Al.SPF) and Flavonoids (Al.FLVF) were investigated for DPPH, ABTS and H2O2 free radical scavenging activities. Further these extracts were subjected to acetylcholinesterase (AChE) & butyrylcholinesterase (BChE) inhibitory activities using Ellman's assay. Phenolic and Flavonoid contents were determined and expressed in mg Gallic acid GAE/g and Rutin RTE/g of samples respectively. Results: In DPPH free radicals scavenging assay, Al.FLVF, Al.SPF and Al.MeF showed highest activity causing 89.41 +/- 0.55, 83.37 +/- 0.34 and 83.37 +/- 0.34% inhibition of free radicals respectively at 1 mg/mL concentration. IC50 for these fractions were 33, 83 and 82 mu g/mL respectively. Similarly, plant extracts showed high ABTS scavenging potential, i.e. Al.FLVF (90.34 +/- 0.55), Al.CfF (83.42 +/- 0.57), Al.MeF (81.49 +/- 0.60) with IC50 of 30, 190 and 70 mu g/ml respectively. further, H2O2 percent scavenging was highly appraised in Al.FLVF (91.29 +/- 0.53, IC50 75), Al.SPF (85.35 +/- 0.61, IC50 70) and Al.EaF (83.48 +/- 0.67, IC50 270 mu g/mL). All fractions exhibited concentration dependent AChE inhibitory activity as; Al.FLVF, 88.31 +/- 0.57 (IC50 70 mu g/mL), Al.SPF, 84.36 +/- 0.64 (IC50 90 mu g/mL), Al.MeF, 78.65 +/- 0.70 (IC50 280 mu g/mL), Al.EaF, 77.45 +/- 0.46 (IC50 270 mu g/mL) and Al.WtF 72.44 +/- 0.58 (IC50 263 mu g/mL) at 1 mg/mL. Likewise the percent BChE inhibitory activity was most obvious in Al.FLVF 85.46 +/- 0.62 (IC50 100 mu g/mL), Al.CfF 83.49 +/- 0.46 (IC50 160 mu g/mL), Al.MeF 82.68 +/- 0.60 (IC50 220 mu g/mL) and Al.SPF 80.37 +/- 0.54 (IC50 120 mu g/mL). Conclusions: These results stipulate that A. laciniata is enriched with phenolic and flavonoid contents that possess significant antioxidant and anticholinestrase effects. This provide pharmacological basis for the presence of compounds that may be effective in Alzheimer's and other neurological disorders. |
Author Address | [Kamal, Zul] Shaheed Benazir Butto Univ, Dept Pharm, Sheringal 18000, Kpk, Pakistan; [Kamal, Zul; Ullah, Farhat; Ayaz, Muhammad; Sadiq, Abdul; Ahmad, Sajjad; Zeb, Anwar; Hussain, Abid; Imran, Muhammad] Univ Malakand, Dept Pharm, Chakdara 18000, Kpk, Pakistan |
Reprint Address | Ayaz, M (reprint author), Univ Malakand, Dept Pharm, Chakdara 18000, Kpk, Pakistan. |
E-mail Address | ayazuop@gmail.com |
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Cited Reference Count | 55 |
Times Cited | 2 |
Total Times Cited Count (WoS, BCI, and CSCD) | 2 |
Publisher | SOC BIOLGIA CHILE |
Publisher City | SANTIAGO |
Publisher Address | CASILLA 16164, SANTIAGO 9, CHILE |
ISSN | 0716-9760 |
29-Character Source Abbreviation | BIOL RES |
ISO Source Abbreviation | Biol. Res. |
Publication Date | APR 1 |
Year Published | 2015 |
Volume | 48 |
Article Number | 21 |
Digital Object Identifier (DOI) | 10.1186/s40659-015-0011-1 |
Page Count | 11 |
Web of Science Category | Biology |
Subject Category | Life Sciences & Biomedicine - Other Topics |
Document Delivery Number | CL6NJ |
Unique Article Identifier | WOS:000357083500001 |
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