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Version 3.20
Publication Type J
Authors Kamal, Z; Ullah, F; Ayaz, M; Sadiq, A; Ahmad, S; Zeb, A; Hussain, A; Imran, M
Author Full Name Kamal, Zul; Ullah, Farhat; Ayaz, Muhammad; Sadiq, Abdul; Ahmad, Sajjad; Zeb, Anwar; Hussain, Abid; Imran, Muhammad
Title Anticholinesterse and antioxidant investigations of crude extracts, subsequent fractions, saponins and flavonoids of atriplex laciniata L.: potential effectiveness in Alzheimer's and other neurological disorders
Language English
Document Type Article
Author Keywords Phenolic & flavonoid contents; DPPH; ABTS; H2O2; Gallic acid; IC50 and anticholinesterase
Abstract Background: Atriplex laciniata L. was investigated for phenolic, flavonoid contents, antioxidant, anticholinesterase activities, in an attempt to explore its effectiveness in Alzheimer's and other neurological disorders. Plant crude methanolic extract (Al.MeF), subsequent fractions; n-hexane (Al.HxF), chloroform (Al.CfF), ethyl acetate (Al.EaF), aqueous (Al.WtF), Saponins (Al.SPF) and Flavonoids (Al.FLVF) were investigated for DPPH, ABTS and H2O2 free radical scavenging activities. Further these extracts were subjected to acetylcholinesterase (AChE) & butyrylcholinesterase (BChE) inhibitory activities using Ellman's assay. Phenolic and Flavonoid contents were determined and expressed in mg Gallic acid GAE/g and Rutin RTE/g of samples respectively. Results: In DPPH free radicals scavenging assay, Al.FLVF, Al.SPF and Al.MeF showed highest activity causing 89.41 +/- 0.55, 83.37 +/- 0.34 and 83.37 +/- 0.34% inhibition of free radicals respectively at 1 mg/mL concentration. IC50 for these fractions were 33, 83 and 82 mu g/mL respectively. Similarly, plant extracts showed high ABTS scavenging potential, i.e. Al.FLVF (90.34 +/- 0.55), Al.CfF (83.42 +/- 0.57), Al.MeF (81.49 +/- 0.60) with IC50 of 30, 190 and 70 mu g/ml respectively. further, H2O2 percent scavenging was highly appraised in Al.FLVF (91.29 +/- 0.53, IC50 75), Al.SPF (85.35 +/- 0.61, IC50 70) and Al.EaF (83.48 +/- 0.67, IC50 270 mu g/mL). All fractions exhibited concentration dependent AChE inhibitory activity as; Al.FLVF, 88.31 +/- 0.57 (IC50 70 mu g/mL), Al.SPF, 84.36 +/- 0.64 (IC50 90 mu g/mL), Al.MeF, 78.65 +/- 0.70 (IC50 280 mu g/mL), Al.EaF, 77.45 +/- 0.46 (IC50 270 mu g/mL) and Al.WtF 72.44 +/- 0.58 (IC50 263 mu g/mL) at 1 mg/mL. Likewise the percent BChE inhibitory activity was most obvious in Al.FLVF 85.46 +/- 0.62 (IC50 100 mu g/mL), Al.CfF 83.49 +/- 0.46 (IC50 160 mu g/mL), Al.MeF 82.68 +/- 0.60 (IC50 220 mu g/mL) and Al.SPF 80.37 +/- 0.54 (IC50 120 mu g/mL). Conclusions: These results stipulate that A. laciniata is enriched with phenolic and flavonoid contents that possess significant antioxidant and anticholinestrase effects. This provide pharmacological basis for the presence of compounds that may be effective in Alzheimer's and other neurological disorders.
Author Address [Kamal, Zul] Shaheed Benazir Butto Univ, Dept Pharm, Sheringal 18000, Kpk, Pakistan; [Kamal, Zul; Ullah, Farhat; Ayaz, Muhammad; Sadiq, Abdul; Ahmad, Sajjad; Zeb, Anwar; Hussain, Abid; Imran, Muhammad] Univ Malakand, Dept Pharm, Chakdara 18000, Kpk, Pakistan
Reprint Address Ayaz, M (reprint author), Univ Malakand, Dept Pharm, Chakdara 18000, Kpk, Pakistan.
E-mail Address ayazuop@gmail.com
Cited References Akhondzadeh Shahin, 2006, Am J Alzheimers Dis Other Demen, V21, P113, DOI 10.1177/153331750602100211; Wang R, 2006, ACTA PHARMACOL SIN, V27, P1, DOI 10.1111/j.1745-7254.2006.00255.x; Howes MJR, 2003, PHYTOTHER RES, V17, P1, DOI 10.1002/ptr.1280; Howes MJR, 2003, PHARMACOL BIOCHEM BE, V75, P513, DOI 10.1016/S0091-3057(03)00128-X; Perry NSL, 2000, J PHARM PHARMACOL, V52, P895, DOI 10.1211/0022357001774598; Ayaz M, 2014, BMC COMPLEM ALTERN M, V14, DOI 10.1186/1472-6882-14-145; Reddy PH, 2005, BRAIN RES REV, V49, P618, DOI 10.1016/j.brainresrev.2005.03.004; Anekonda TS, 2005, BRAIN RES REV, V50, P361, DOI 10.1016/j.brainresrev.2005.09.001; KNAPP MJ, 1994, JAMA-J AM MED ASSOC, V271, P985, DOI 10.1001/jama.271.13.985; Kim DO, 2003, FOOD CHEM, V81, P321, DOI 10.1016/S0308-8146(02)00423-5; Ha SK, 2012, NEUROPHARMACOLOGY, V63, P211, DOI 10.1016/j.neuropharm.2012.03.016; REINIKAINEN KJ, 1990, J NEUROSCI RES, V27, P576, DOI 10.1002/jnr.490270419; De Strooper B, 2000, J CELL SCI, V113, P1857; Ayaz M, 2014, BMC COMPLEM ALTERN M, V14, DOI 10.1186/1472-6882-14-465; McGeer PL, 2007, NEUROBIOL AGING, V28, P639, DOI 10.1016/j.neurobiolaging.2006.03.013; Pietta PG, 2000, J NAT PROD, V63, P1035, DOI 10.1021/np9904509; Shah SMH, 2015, PAK J PHARM SCI, V28, P23; Amel O. H., 2013, Asian Pacific Journal of Tropical Disease, V3, P367, DOI 10.1016/S2222-1808(13)60086-9; Park HH, 2008, ARCH PHARM RES, V31, P1303, DOI 10.1007/s12272-001-2110-5; DeKosky ST, 2008, JAMA-J AM MED ASSOC, V300, P2253, DOI 10.1001/jama.2008.683; Zeb A, 2014, BIOL RES, V47, DOI 10.1186/0717-6287-47-76; SHAHIDI F, 1992, CRIT REV FOOD SCI, V32, P67; Tildesley NTJ, 2003, PHARMACOL BIOCHEM BE, V75, P669, DOI 10.1016/S0091-3057(03)00122-9; Refolo LM, 2001, NEUROBIOL DIS, V8, P890, DOI 10.1006/nbdi.2001.0422; Zahran MA, 2010, PLANT VEG, V4, P249, DOI 10.1007/978-90-481-8595-5_4; Re R, 1999, FREE RADICAL BIO MED, V26, P1231, DOI 10.1016/S0891-5849(98)00315-3; Rinaldi P, 2003, NEUROBIOL AGING, V24, P915, DOI 10.1016/S0197-4580(03)00031-9; Thatoi HN, 2014, ACTA PHYSIOL PLANT, V36, P561, DOI 10.1007/s11738-013-1438-z; Szilagyi G, 2005, J NEUROL SCI, V229, P275, DOI 10.1016/j.jns.2004.11.053; PALMER AM, 1987, J NEUROCHEM, V48, P8, DOI 10.1111/j.1471-4159.1987.tb13120.x; Debnath T, 2011, FOOD CHEM, V128, P697, DOI 10.1016/j.foodchem.2011.03.090; Zeb A, 2014, BIOL RES, V47, DOI 10.1186/0717-6287-47-57; Lopes DVS, 2004, EUR J PHARMACOL, V495, P87, DOI 10.1016/j.ejphar.2004.05.026; Allain H, 2003, FUND CLIN PHARMACOL, V17, P419, DOI 10.1046/j.1472-8206.2003.00153.x; Hagerman AE, 1998, J AGR FOOD CHEM, V46, P1887, DOI 10.1021/jf970975b; DAVIES P, 1980, NATURE, V288, P279, DOI 10.1038/288279a0; Yasar S, 2005, NEUROBIOL AGING, V26, P157, DOI 10.1016/j.neurobiolaging.2004.03.009; Moyo M, 2010, FOOD CHEM, V123, P69, DOI 10.1016/j.foodchem.2010.03.130; Lashuel HA, 2002, NATURE, V418, P291, DOI 10.1038/418291a; Trevisan MTS, 2003, QUIM NOVA, V26, P301, DOI 10.1590/S0100-40422003000300002; Lee ST, 2008, ALZ DIS ASSOC DIS, V22, P222, DOI 10.1097/WAD.0b013e31816c92e6; BRANDWILLIAMS W, 1995, FOOD SCI TECHNOL-LEB, V28, P25; RUCH RJ, 1989, CARCINOGENESIS, V10, P1003, DOI 10.1093/carcin/10.6.1003; Heinrich M, 2004, J ETHNOPHARMACOL, V92, P147, DOI 10.1016/S0378-8741(04)00073-X; Barlow SM, 1990, FOOD ANTIOXIDANTS, P253; DAVIES P, 1976, LANCET, V2, P1403; ELLMAN GL, 1961, BIOCHEM PHARMACOL, V7, P88, DOI 10.1016/0006-2952(61)90145-9; Ichikawa H, 2001, J MED FOOD, V4, P211, DOI 10.1089/10966200152744481; Imran M, 2014, PHARMACOLOGYONLINE, V3, P26; Mikaili Peyman, 2012, Asian Pacific Journal of Tropical Biomedicine, V2, pS1189; Shah SMM, 2014, BMC COMPLEM ALTERN M, V14, DOI 10.1186/1472-6882-14-181; Shah SMM, 2014, BIOL RES, V47, DOI 10.1186/0717-6287-47-65; Sharma GN, 2011, INT J PHARM CHEM RES, V2, P27; Tundis R, 2009, J ENZYM INHIB MED CH, V24, P818, DOI 10.1080/14756360802399662; Voet D, 1995, BIOCHEMISTRY-US, P390
Cited Reference Count 55
Times Cited 2
Total Times Cited Count (WoS, BCI, and CSCD) 2
Publisher City SANTIAGO
Publisher Address CASILLA 16164, SANTIAGO 9, CHILE
ISSN 0716-9760
29-Character Source Abbreviation BIOL RES
ISO Source Abbreviation Biol. Res.
Publication Date APR 1
Year Published 2015
Volume 48
Article Number 21
Digital Object Identifier (DOI) 10.1186/s40659-015-0011-1
Page Count 11
Web of Science Category Biology
Subject Category Life Sciences & Biomedicine - Other Topics
Document Delivery Number CL6NJ
Unique Article Identifier WOS:000357083500001
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