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Publication Type J
Authors Kamal, Z; Ullah, F; Ayaz, M; Sadiq, A; Ahmad, S; Zeb, A; Hussain, A; Imran, M
Author Full Name Kamal, Zul; Ullah, Farhat; Ayaz, Muhammad; Sadiq, Abdul; Ahmad, Sajjad; Zeb, Anwar; Hussain, Abid; Imran, Muhammad
Title Anticholinesterse and antioxidant investigations of crude extracts, subsequent fractions, saponins and flavonoids of atriplex laciniata L.: potential effectiveness in Alzheimer's and other neurological disorders
Source BIOLOGICAL RESEARCH
Language English
Document Type Article
Author Keywords Phenolic & flavonoid contents; DPPH; ABTS; H2O2; Gallic acid; IC50 and anticholinesterase
Keywords Plus RANDOMIZED CONTROLLED-TRIAL; TOTAL PHENOLIC CONTENTS; POLYGONUM-HYDROPIPER L; TRADITIONAL MEDICINE; METHANOLIC EXTRACT; DISEASE; ACETYLCHOLINESTERASE; PLANTS; CHINESE; PHYTOCHEMICALS
Abstract Background: Atriplex laciniata L. was investigated for phenolic, flavonoid contents, antioxidant, anticholinesterase activities, in an attempt to explore its effectiveness in Alzheimer's and other neurological disorders. Plant crude methanolic extract (Al.MeF), subsequent fractions; n-hexane (Al.HxF), chloroform (Al.CfF), ethyl acetate (Al.EaF), aqueous (Al.WtF), Saponins (Al.SPF) and Flavonoids (Al.FLVF) were investigated for DPPH, ABTS and H2O2 free radical scavenging activities. Further these extracts were subjected to acetylcholinesterase (AChE) & butyrylcholinesterase (BChE) inhibitory activities using Ellman's assay. Phenolic and Flavonoid contents were determined and expressed in mg Gallic acid GAE/g and Rutin RTE/g of samples respectively. Results: In DPPH free radicals scavenging assay, Al.FLVF, Al.SPF and Al.MeF showed highest activity causing 89.41 +/- 0.55, 83.37 +/- 0.34 and 83.37 +/- 0.34% inhibition of free radicals respectively at 1 mg/mL concentration. IC50 for these fractions were 33, 83 and 82 mu g/mL respectively. Similarly, plant extracts showed high ABTS scavenging potential, i.e. Al.FLVF (90.34 +/- 0.55), Al.CfF (83.42 +/- 0.57), Al.MeF (81.49 +/- 0.60) with IC50 of 30, 190 and 70 mu g/ml respectively. further, H2O2 percent scavenging was highly appraised in Al.FLVF (91.29 +/- 0.53, IC50 75), Al.SPF (85.35 +/- 0.61, IC50 70) and Al.EaF (83.48 +/- 0.67, IC50 270 mu g/mL). All fractions exhibited concentration dependent AChE inhibitory activity as; Al.FLVF, 88.31 +/- 0.57 (IC50 70 mu g/mL), Al.SPF, 84.36 +/- 0.64 (IC50 90 mu g/mL), Al.MeF, 78.65 +/- 0.70 (IC50 280 mu g/mL), Al.EaF, 77.45 +/- 0.46 (IC50 270 mu g/mL) and Al.WtF 72.44 +/- 0.58 (IC50 263 mu g/mL) at 1 mg/mL. Likewise the percent BChE inhibitory activity was most obvious in Al.FLVF 85.46 +/- 0.62 (IC50 100 mu g/mL), Al.CfF 83.49 +/- 0.46 (IC50 160 mu g/mL), Al.MeF 82.68 +/- 0.60 (IC50 220 mu g/mL) and Al.SPF 80.37 +/- 0.54 (IC50 120 mu g/mL). Conclusions: These results stipulate that A. laciniata is enriched with phenolic and flavonoid contents that possess significant antioxidant and anticholinestrase effects. This provide pharmacological basis for the presence of compounds that may be effective in Alzheimer's and other neurological disorders.
Author Address [Kamal, Zul] Shaheed Benazir Butto Univ, Dept Pharm, Sheringal 18000, Kpk, Pakistan; [Kamal, Zul; Ullah, Farhat; Ayaz, Muhammad; Sadiq, Abdul; Ahmad, Sajjad; Zeb, Anwar; Hussain, Abid; Imran, Muhammad] Univ Malakand, Dept Pharm, Chakdara 18000, Kpk, Pakistan
Reprint Address Ayaz, M (reprint author), Univ Malakand, Dept Pharm, Chakdara 18000, Kpk, Pakistan.
E-mail Address ayazuop@gmail.com
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Cited Reference Count 55
Times Cited 2
Total Times Cited Count (WoS, BCI, and CSCD) 2
Publisher SOC BIOLGIA CHILE
Publisher City SANTIAGO
Publisher Address CASILLA 16164, SANTIAGO 9, CHILE
ISSN 0716-9760
29-Character Source Abbreviation BIOL RES
ISO Source Abbreviation Biol. Res.
Publication Date APR 1
Year Published 2015
Volume 48
Article Number 21
Digital Object Identifier (DOI) 10.1186/s40659-015-0011-1
Page Count 11
Web of Science Category Biology
Subject Category Life Sciences & Biomedicine - Other Topics
Document Delivery Number CL6NJ
Unique Article Identifier WOS:000357083500001
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