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Publication Type J
Authors Singh, D., N. S. Yadav, V. Tiwari, P. K. Agarwal and B. Jha
Title A SNARE-Like Superfamily Protein SbSLSP from the Halophyte Salicornia brachiata Confers Salt and Drought Tolerance by Maintaining Membrane Stability, K+/Na+ Ratio, and Antioxidant Machinery
Source Frontiers in Plant Science
Author Keywords abiotic stress SNARE-like superfamily protein clathrin adaptor protein complex clathrin-coated vesicles halophyte Salicornia brachiata salt-inducible gene extreme halophyte abiotic stress arabidopsis-thaliana transcription factors transgenic tobacco hydrogen-peroxide oxidative stress plant-growth water-stress e. coli
Abstract About 1000 salt-responsive ESTs were identified from an extreme halophyte Salicornia brachiata. Among these, a novel salt-inducible gene SbSLSP (alicornia brachiata SNARE-like superfamily protein), showed up-regulation upon salinity and dehydration stress. The presence of cis regulatory motifs related to abiotic stress in the putative promoter region supports our finding that SbSLSP gene is inducible by abiotic stress. The SbSLSP protein showed a high sequence identity to hypothetical/uncharacterized proteins from Beta vulgaris, Spinacia oleracea, Eucalyptus grandis, and Prunus persica and with SNARE-like superfamily proteins from Zostera marina and Arabidopsis thaliana. Bioinformatics analysis predicted a clathrin adaptor complex small-chain domain and N-myristoylation site in the SbSLSP protein. Subcellular localization studies indicated that the SbSLSP protein is mainly localized in the plasma membrane. Using transgenic tobacco lines, we establish that overexpression of SbSLSP resulted in elevated tolerance to salt and drought stress. The improved tolerance was confirmed by alterations in a range of physiological parameters, including high germination and survival rate, higher leaf chlorophyll contents, and reduced accumulation of Na+ ion and reactive oxygen species (ROS). Furthermore, overexpressing lines also showed lower water loss, higher cell membrane stability, and increased accumulation of proline and ROS-scavenging enzymes. Overexpression of SbSLSP also enhanced the transcript levels of ROS-scavenging and signaling enzyme genes. This study is the first investigation of the function of the SbSLSP gene as a novel determinant of salinity/drought tolerance. The results suggest that SbSLSP could be a potential candidate to increase salinity and drought tolerance in crop plants for sustainable agriculture in semi-arid saline soil.
Author Address [Singh, Dinkar; Yadav, Narendra Singh; Tiwari, Vivekanand; Agarwal, Pradeep K.; Jha, Bhavanath] Cent Salt & Marine Chem Res Inst, CSIR, Div Marine Biotechnol & Ecol, Bhavnagar 364002, Gujarat, India. [Agarwal, Pradeep K.; Jha, Bhavanath] CSIR, Acad Sci & Innovat Res, New Delhi 110001, India. Jha, B (reprint author), Cent Salt & Marine Chem Res Inst, CSIR, Div Marine Biotechnol & Ecol, Bhavnagar 364002, Gujarat, India.; Jha, B (reprint author), CSIR, Acad Sci & Innovat Res, New Delhi 110001, India. bjha@csmcri.org
ISSN 1664-462X
ISBN 1664-462X
29-Character Source Abbreviation Front. Plant Sci.
Publication Date Jun
Year Published 2016
Volume 7
Digital Object Identifier (DOI) 10.3389/fpls.2016.00737
Unique Article Identifier WOS:000376860700001
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