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| Publication Type | J |
| Authors | Arbi, S. B., D. Chekireb, P. Quatrini, V. Catania, D. Cheriet and A. Ouartsi |
| Title | Phenotypic and genotypic characterization of root nodules rhizobia of Medicago littoralis Rhode and Melilotus indicus (L.) All. growing in the Oasis of Touggourt, Oued Righ Valley, in the Algerian Sahara |
| Source | Symbiosis |
| Author Keywords | Rhizobia Medicago Melilotus Oasis soil Phenotype Genotype sp-nov sinorhizobium-meliloti phaseolus-vulgaris nitrogen-fixation sativa diversity soils symbionts ensifer tunisia |
| Abstract | Forty rhizobial strains were isolated from root nodules of Medicago littoralis Rhode and Melilotus indicus (L.) harvested from the sandy soils of Touggourt's oases in the Oued Righ Valley, Algerian Sahara. The isolates were studied for their cultural, biochemical and symbiotic effectiveness. All of them were fast-growing bacteria; utilized a wide range of carbon sources, produced abundant extracellular polysaccharides, tolerated high concentrations of NaCl (up to 2.5 %), grew at temperatures between 28 and 45 A degrees C and at pH values between 4.5 and 9. The isolates were sensitive to the antibiotics kanamycin, tetracycline and rifampicin but showed resistance to neomycin and erythromycin. All the isolates induced the formation of effective nodules on their host plants. On the basis of the physiological, biochemical and symbiotic effectiveness, we selected six strains MD05, MD09, MD12, ML08, ML17 and ML22 for genotypic characterization. Phylogenetic analysis of the selected strains based on 16S ribosomal RNA gene showed that these strains of bacteria were affiliated to the Ensifer meliloti group. |
| Author Address | [Arbi, Souad Baba; Chekireb, Djamel; Cheriet, Dahbia; Ouartsi, Akila] Univ Badji Mokhtar Annaba, Fac Sci, Dept Biochem, Lab Appl Biochem & Microbiol, Sidi Amar 23200, Annaba, Algeria. [Quatrini, Paola; Catania, Valentina] Univ Palermo, Dept Biol Chem & Pharmaceut Sci & Technol STEBICE, I-90128 Palermo, Italy. Arbi, SB (reprint author), Univ Badji Mokhtar Annaba, Fac Sci, Dept Biochem, Lab Appl Biochem & Microbiol, BP 12, Sidi Amar 23200, Annaba, Algeria. souadbabaarbi@gmail.com |
| ISSN | 0334-5114 |
| ISBN | 0334-5114 |
| 29-Character Source Abbreviation | Symbiosis |
| Publication Date | Jun |
| Year Published | 2015 |
| Volume | 66 |
| Issue | 2 |
| Beginning Page | 75-87 |
| Digital Object Identifier (DOI) | 10.1007/s13199-015-0336-0 |
| Unique Article Identifier | WOS:000362591700003 |
| Plants associated with this reference |