Loading content, please wait..
loading..
Logo
Version 3.21
or
Publication Type J
Authors Barkla, B. J., R. Vera-Estrella and C. Raymond
Title Single-cell-type quantitative proteomic and ionomic analysis of epidermal bladder cells from the halophyte model plant Mesembryanthemum crystallinum to identify salt-responsive proteins
Source Bmc Plant Biology
Author Keywords Proteomics Trichome Salinity Salt tolerance Crassulacean acid metabolism (CAM) Ionomics Chloride Sodium V-ATPase Single cell-type crassulacean acid metabolism vacuolar h+-atpase label-free shotgun proteomics salinity tolerance transport metabolomics arabidopsis expression trichome
Abstract Background: Epidermal bladder cells (EBC) are large single-celled, specialized, and modified trichomes found on the aerial parts of the halophyte Mesembryanthemum crystallinum. Recent development of a simple but high throughput technique to extract the contents from these cells has provided an opportunity to conduct detailed single-cell-type analyses of their molecular characteristics at high resolution to gain insight into the role of these cells in the salt tolerance of the plant. Results: In this study, we carry out large-scale complementary quantitative proteomic studies using both a label (DIGE) and label-free (GeLC-MS) approach to identify salt-responsive proteins in the EBC extract. Additionally we perform an ionomics analysis (ICP-MS) to follow changes in the amounts of 27 different elements. Using these methods, we were able to identify 54 proteins and nine elements that showed statistically significant changes in the EBC from salt-treated plants. GO enrichment analysis identified a large number of transport proteins but also proteins involved in photosynthesis, primary metabolism and Crassulacean acid metabolism (CAM). Validation of results by western blot, confocal microscopy and enzyme analysis helped to strengthen findings and further our understanding into the role of these specialized cells. As expected EBC accumulated large quantities of sodium, however, the most abundant element was chloride suggesting the sequestration of this ion into the EBC vacuole is just as important for salt tolerance. Conclusions: This single-cell type omics approach shows that epidermal bladder cells of M. crystallinum are metabolically active modified trichomes, with primary metabolism supporting cell growth, ion accumulation, compatible solute synthesis and CAM. Data are available via ProteomeXchange with identifier PXD004045.
Author Address [Barkla, Bronwyn J.; Raymond, Carolyn] So Cross Univ, Southern Cross Plant Sci, Lismore, NSW 2480, Australia. [Vera-Estrella, Rosario] Univ Nacl Autonoma Mexico, Inst Biotecnol, Cuernavaca 62191, Morelos, Mexico. Barkla, BJ (reprint author), So Cross Univ, Southern Cross Plant Sci, Lismore, NSW 2480, Australia. bronwyn.barkla@scu.edu.au
ISSN 1471-2229
ISBN 1471-2229
29-Character Source Abbreviation BMC Plant Biol.
Publication Date May
Year Published 2016
Volume 16
Digital Object Identifier (DOI) 10.1186/s12870-016-0797-1
Unique Article Identifier WOS:000375856000002
Plants associated with this reference

LEGAL NOTICES — This website is protected by Copyright © The University of Sussex, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021. The eHALOPH database is protected by Database Right and Copyright © The University of Sussex and other contributors, 2006, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021. This database is based on an earlier work by James Aronson.
THIS WEBSITE AND THIS DATABASE ARE PROVIDED ON AN "AS IS" BASIS, AND YOU USE THEM AND RELY ON THEM AT YOUR OWN RISK.

Contact email: halophytes@sussex.ac.uk
Credits – Tim Flowers, Joaquim Santos, Moritz Jahns, Brian Warburton, Peter Reed