Loading content, please wait..
loading..
Logo
Version 3.24
or
Publication Type J
Authors Nishiuchi, S; Liu, S; Takano, T
Author Full Name Nishiuchi, Shunsaku; Liu, Shenkui; Takano, Tetsuo
Title Isolation and characterization of a metallothionein-1 protein in Chloris virgata Swartz that enhances stress tolerances to oxidative, salinity and carbonate stress in Saccharomyces cerevisiaei
Source BIOTECHNOLOGY LETTERS
Language English
Document Type Article
Author Keywords alkaline salt; Chloris virgata; metallothionein; reactive oxidant species; salinity
Keywords Plus TYPE-2 METALLOTHIONEIN; ESCHERICHIA-COLI; EXPRESSION; RICE; SEEDLINGS; L.
Abstract Chloris virgata Swartz (C. virgata) is a gramineous wild plant that is found in alkaline soil areas in northeast China and is highly tolerant to carbonate stress. We constructed a cDNA library from C. virgata seedlings treated with NaHCO3, and isolated a type1 metallothionein (MT1) gene (ChlMT1: AB294238) from the library. The amino acid sequence of ChlMT1 contained 12 cysteine residues that constituted the Cys-X-Cys (X = amino acid except Cys) motifs in the N- and C-terminal regions. Northern hybridization showed that expression of ChlMT1 was induced by several abiotic stresses, from salts (NaCl and NaHCO3), a ROS inducer (paraquat), and metals (CUSO4, ZnSO4, and COCl2). ChlMT1 expression in leaf was induced by 200 mM NaCl and 100 mM NaHCO3. About 5 mu M Paraquat, 500 mu M Zn2+, and 500 mu M Co2+ also induced expression of ChlMT1 in leaf after 6 h, and 100 mu M CU2+ induced it after 24 h. Saccharomyces cerevisiae when transformed with the ChlMT1 gene had dramatically increased tolerances to salts (NaCl and NaHCO3) and ROS.
Author Address Univ Tokyo, ANESC, Tokyo 1880002, Japan; NE Forestry Univ, ASNESC, Stress Mol Biol Lab, Harbin 150040, Peoples R China
Reprint Address Takano, T (reprint author), Univ Tokyo, ANESC, 1-1-1 Midori Cho, Tokyo 1880002, Japan.
E-mail Address takano@anesc.u-tokyo.ac.jp
Cited References Huang CH, 2005, J EXP BOT, V56, P3041, DOI 10.1093/jxb/eri301; Mir G, 2004, J EXP BOT, V55, P2483, DOI 10.1093/jxb/erh254; Cobbett C, 2002, ANNU REV PLANT BIOL, V53, P159, DOI 10.1146/annurev.arplant.53.100301.135154; Takahashi R, 2007, BIOTECHNOL LETT, V29, P501, DOI 10.1007/s10529-006-9246-9; ROBINSON NJ, 1993, BIOCHEM J, V295, P1; Zhou GK, 2006, J BIOCHEM MOL BIOL, V39, P595; Navabpour S, 2003, J EXP BOT, V54, P2285, DOI 10.1093/jxb/erg267; Akashi K, 2004, BIOCHEM BIOPH RES CO, V323, P72, DOI 10.1016/j.bbrc.2004.08.056; Wong HL, 2004, PLANT PHYSIOL, V135, P1447, DOI 10.1104/pp.103.036384; Guo J.X., 1998, J NE NORMAL U, V2, P61; Jin SM, 2006, BIOTECHNOL LETT, V28, P1749, DOI 10.1007/s10529-006-9152-1; LIU S, 2006, ILLUSTRATED BOOK WIL; Menezes-Benavente L, 2004, REDOX REP, V9, P29, DOI 10.1179/135100004225003888; An ZG, 2006, J EXP BOT, V57, P3575, DOI 10.1093/jxb/erl102
Cited Reference Count 14
Times Cited 15
Total Times Cited Count (WoS, BCI, and CSCD) 23
Publisher SPRINGER
Publisher City DORDRECHT
Publisher Address VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
ISSN 0141-5492
29-Character Source Abbreviation BIOTECHNOL LETT
ISO Source Abbreviation Biotechnol. Lett.
Publication Date AUG
Year Published 2007
Volume 29
Issue 8
Beginning Page 1301
Ending Page 1305
Digital Object Identifier (DOI) 10.1007/s10529-007-9396-4
Page Count 5
Web of Science Category Biotechnology & Applied Microbiology
Subject Category Biotechnology & Applied Microbiology
Document Delivery Number 192SZ
Unique Article Identifier WOS:000248224600024
Plants associated with this reference

LEGAL NOTICES — This website is protected by Copyright © The University of Sussex, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022. The eHALOPH database is protected by Database Right and Copyright © The University of Sussex and other contributors, 2006, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022. This database is based on an earlier work by James Aronson.
THIS WEBSITE AND THIS DATABASE ARE PROVIDED ON AN "AS IS" BASIS, AND YOU USE THEM AND RELY ON THEM AT YOUR OWN RISK.

Contact email: halophytes@sussex.ac.uk
Credits – Tim Flowers, Joaquim Santos, Moritz Jahns, Brian Warburton, Peter Reed