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Publication Type J
Authors Mohamed, E., R. Matsuda, A. A. El-khatib, K. Takechi, H. Takano and S. Takio
Title Characterization of the superoxide dismutase genes of the halophyte Suaeda maritima in Japan and Egypt
Source Plant Cell Reports
Author Keywords Halophyte Iron Salt stress Superoxide dismutase Suaeda maritima salt tolerance natural halophyte stress tolerance transgenic rice salinity plants nacl arabidopsis enzymes growth
Abstract Suaeda maritima varieties native to Japan and Egypt were cultured under aseptic conditions. The varieties differed in genetic distance but exhibited similar expression profiles of superoxide dismutase isozyme genes. The expression characteristics of superoxide dismutase (SOD; EC 1.15.1.1) isozyme genes from halophytic Suaeda marit ima plants native to Japan and Egypt were analyzed using young plants grown under aseptic conditions. A phylogenetic tree based on internal transcribed spacer sequences suggested that Egyptian S. maritima is related to European and India S. maritima, while Japanese S. maritima belongs to a separate clade. An in-gel SOD activity staining assay revealed that leaves from both the Egyptian and Japanese varieties showed high levels of CuZn-SOD and Fe-SOD activity, but no Mn-SOD activity; conversely, stems from both varieties showed Mn-SOD activity as well as other SOD isozyme activities. In Japanese S. maritima leaves, SOD activity was increased by incubation in growth medium containing 400 mM NaCl, while Egyptian S. maritima leaves showed elevated SOD activity in the absence of high salt. Genes encoding Mn-SOD and Fe-SOD were isolated from both plant types. RT-PCR analysis revealed that all SOD isozyme-encoding genes were expressed at the same levels in leaves from both plant types grown in normal or high-salt medium. In contrast, the expression of genes encoding choline monooxygenase and betaine aldehyde dehydrogenase, which are involved in betacyanin biosynthesis, was increased in high-salt medium. In leaves of Japanese S. maritima plants, Fe deficiency without high salt exposure preferentially decreased Fe-SOD activity. On the other hand, Fe deficiency with high salt exposure decreased not only Fe-SOD activity but also CuZn-SOD activity, suggesting that Fe availability is involved in the up-regulation of SOD isozymes mediating salt tolerance.
Author Address [Mohamed, Elsayed; Takio, Susumu] Kumamoto Univ, Ctr Marine Environm Studies, Kurokami, Kumamoto 8608555, Japan. [Mohamed, Elsayed] Al Azhar Univ, Fac Sci, Assiut, Egypt. [Matsuda, Ryuya; Takechi, Katsuaki; Takano, Hiroyoshi] Kumamoto Univ, Grad Sch Sci & Technol, Kurokami, Kumamoto 8608555, Japan. [El-khatib, Ahmed A.] Sohag Univ, Fac Sci, Sohag 82524, Egypt. Takio, S (reprint author), Kumamoto Univ, Ctr Marine Environm Studies, Kurokami, Kumamoto 8608555, Japan. stakio@gpo.kumamoto-u.ac.jp
ISSN 0721-7714
ISBN 0721-7714
Publication Date Dec
Year Published 2015
Volume 34
Issue 12
Beginning Page 2099-2110
Digital Object Identifier (DOI) 10.1007/s00299-015-1854-1
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