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Publication Type J
Authors Chamkouri, N., S. Khodadoust and F. Ghalavandi
Title Solid-Phase Extraction Coupled with HPLC-DAD for Determination of B Vitamin Concentrations in Halophytes
Source Journal of Chromatographic Science
Author Keywords performance liquid-chromatography water-soluble vitamins capillary-electrophoresis salinity
Abstract In our study, a simple and sensitive method was proposed for determination of vitamins B group in leaves of Suaeda aegyptiaca and Suaeda Vera (Halophytes) by HPLC-DAD with solid-phase extraction (SPE) as the preconcentration step. The variables affecting the extraction and determination conditions were investigated and optimized. A preconcentration step based on SPE is necessary to remove interfering components. The limit of detection obtained was <5 mg kg(-1), the relative standard deviation (RSD) was <4%, and the recovery was in the range of 91-102%. The linear correlation coefficient was 0.999. The method was successfully applied to determine group B vitamins in the halophyte samples. As a whole, these findings may confirm the interesting potential of this halophyte as a valuable source of natural bioactive molecules.
Author Address [Chamkouri, Narges; Ghalavandi, Forogh] Abadan Sch Med Sci, Abadan, Iran. [Khodadoust, Saeid] Behbahan Khatam Alanbia Univ Technol, Dept Chem, Behbahan, Iran. Chamkouri, N (reprint author), Abadan Sch Med Sci, Abadan, Iran. narges.chamkouri@gmail.com
ISSN 0021-9665
ISBN 0021-9665
Publication Date Nov-Dec
Year Published 2015
Volume 53
Issue 10
Beginning Page 1720-1724
Digital Object Identifier (DOI) 10.1093/chromsci/bmv080
Unique Article Identifier WOS:000368427200014
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