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Publication Type J
Authors Alves, A., R. Riesco, A. Correia and M. E. Trujillo
Title Microbacterium proteolyticum sp nov isolated from roots of Halimione portulacoides
Source International Journal of Systematic and Evolutionary Microbiology
Author Keywords redefined genus microbacterium deoxyribonucleic-acid lipid-composition orla-jensen reclassification identification chromatography hybridization endophytes sequences
Abstract An endophytic actinobacterial strain RZ36(T), isolated from roots of the salt-marsh plant Halimione portulacoides, was subjected to a polyphasic taxonomic characterization. 16S rRNA gene sequence analysis revealed that this strain belonged to the genus Microbacterium. The closest phylogenetic relative was Microbacterium hominis DSM 12509(T), with a pairwise 16S rRNA gene sequence similarity of 98.8%. The DNA DNA hybridization value between strain RZ36(T) and M. hominis DSM 12509(T) was 16%. The affiliation to the genus Microbacterium was corroborated by phenotypic and chemotaxonomic characteristics. The cell-wall peptidoglycan type was B2 beta and the diagnostic diamino acid was ornithine. Whole-cell sugars detected were galactose, glucose, rhamnose, ribose and xylose. The major fatty acids were anteiso-C-15:0 and iso-C-16:0 and the major menaquinone was MK-11 (64%). Main polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. The genomic DNA G +C content was 69.7 mol%. Thus, on the basis of phenotypic, genotypic and chemotaxonomic data, strain RZ36(T) is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium proteolyticum sp. nov. (type strain RZ36(T)=DSM 27100(T)=CECT 8350(T)) is proposed.
Author Address [Alves, Artur; Correia, Antonio] Univ Aveiro, CESAM, Dept Biol, P-3810193 Aveiro, Portugal. [Riesco, Raul; Trujillo, Martha E.] Univ Salamanca, Lab 214, Dept Microbiol & Genet, Salamanca 37007, Spain. Alves, A (reprint author), Univ Aveiro, CESAM, Dept Biol, Campus Santiago, P-3810193 Aveiro, Portugal. arturalves@ua.pt
ISSN 1466-5026
ISBN 1466-5026
Publication Date Jun
Year Published 2015
Volume 65
Beginning Page 1794-1798
Digital Object Identifier (DOI) 10.1099/ijs.0.000177
Unique Article Identifier WOS:000360029800015
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