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| Publication Type | J |
| Authors | Alves, A., R. Riesco, A. Correia and M. E. Trujillo |
| Title | Microbacterium proteolyticum sp nov isolated from roots of Halimione portulacoides |
| Source | International Journal of Systematic and Evolutionary Microbiology |
| Author Keywords | redefined genus microbacterium deoxyribonucleic-acid lipid-composition orla-jensen reclassification identification chromatography hybridization endophytes sequences |
| Abstract | An endophytic actinobacterial strain RZ36(T), isolated from roots of the salt-marsh plant Halimione portulacoides, was subjected to a polyphasic taxonomic characterization. 16S rRNA gene sequence analysis revealed that this strain belonged to the genus Microbacterium. The closest phylogenetic relative was Microbacterium hominis DSM 12509(T), with a pairwise 16S rRNA gene sequence similarity of 98.8%. The DNA DNA hybridization value between strain RZ36(T) and M. hominis DSM 12509(T) was 16%. The affiliation to the genus Microbacterium was corroborated by phenotypic and chemotaxonomic characteristics. The cell-wall peptidoglycan type was B2 beta and the diagnostic diamino acid was ornithine. Whole-cell sugars detected were galactose, glucose, rhamnose, ribose and xylose. The major fatty acids were anteiso-C-15:0 and iso-C-16:0 and the major menaquinone was MK-11 (64%). Main polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. The genomic DNA G +C content was 69.7 mol%. Thus, on the basis of phenotypic, genotypic and chemotaxonomic data, strain RZ36(T) is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium proteolyticum sp. nov. (type strain RZ36(T)=DSM 27100(T)=CECT 8350(T)) is proposed. |
| Author Address | [Alves, Artur; Correia, Antonio] Univ Aveiro, CESAM, Dept Biol, P-3810193 Aveiro, Portugal. [Riesco, Raul; Trujillo, Martha E.] Univ Salamanca, Lab 214, Dept Microbiol & Genet, Salamanca 37007, Spain. Alves, A (reprint author), Univ Aveiro, CESAM, Dept Biol, Campus Santiago, P-3810193 Aveiro, Portugal. arturalves@ua.pt |
| ISSN | 1466-5026 |
| ISBN | 1466-5026 |
| Publication Date | Jun |
| Year Published | 2015 |
| Volume | 65 |
| Beginning Page | 1794-1798 |
| Digital Object Identifier (DOI) | 10.1099/ijs.0.000177 |
| Unique Article Identifier | WOS:000360029800015 |
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