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Publication Type J
Authors Kang, H., S. Koppula, H. K. Kim and T. K. Park
Title Suaeda japonica Makino Attenuates Lipopolysaccharide-Induced Neuro-Inflammatory Responses in BV-2 Microglia via NF-kappa B Signaling
Source Tropical Journal of Pharmaceutical Research
Author Keywords Suaeda japonica Antioxidant activity Anti-inflammatory activity Microglial Cells iNOS IL-6 nitric-oxide synthase induction
Abstract Purpose: To evaluate in vitro anti-oxidant and anti-neuro-inflammatory activities of Suaeda japonica extract (SJE) in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells. Methods: 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay was used to study the antioxidant effects. 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyl-tetrazolium bromide (MTT) assay was used to study cell viabilities. LPS-stimulated BV- microglial cells were used to study the gene expression and production of inflammatory mediators determined by Western blot analysis. Results: SJE significantly inhibited the DPPH generated free radicals, and suppressed LPS-induced expression of inducible nitric oxide synthase (iNOS) and production of nitric oxide (NO) in a concentration-dependent manner. It decreased LPS-induced expression of some inflammatory mediators and pro-inflammatory cytokines (cyclooxygenase-2 and interleukin (IL)-6). This suppression of inflammatory mediators was nuclear factor kappa-B (NF-kappa B)-dependent. Conclusion: Our findings imply that SJE may be a potential therapeutic agent in regulating microglia-mediated neuroinflammatory responses observed in several neurodegenerative diseases.
Author Address [Kang, Hyun; Koppula, Sushruta; Park, Tae-Kyu] Konkuk Univ, Coll Biomed & Hlth Sci, Res Inst Biomed & Hlth Sci, Dept Biotechnol, Chungju 380701, South Korea. [Kim, Hoi-Ki] Fanipin Korea Co Ltd, Ochang 208211, South Korea. [Kang, Hyun] KuGen Healthcare Inst, Chungju 380150, South Korea. Park, TK (reprint author), Konkuk Univ, Coll Biomed & Hlth Sci, Res Inst Biomed & Hlth Sci, Dept Biotechnol, Chungju 380701, South Korea. tkpark@kku.ac.kr
ISSN 1596-5996
ISBN 1596-5996
Publication Date Jun
Year Published 2013
Volume 12
Issue 3
Beginning Page 351-356
Digital Object Identifier (DOI) 10.4314/tjpr.v12i3.12
Unique Article Identifier WOS:000320760400012
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