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Publication Type J
Authors Shukla, P. S., P. Agarwal, K. Gupta and P. K. Agarwal
Title Molecular characterization of an MYB transcription factor from a succulent halophyte involved in stress tolerance
Source Aob Plants
Author Keywords Abiotic stress halophyte R2R3-MYB Salicornia brachiata transcription factor yeast salicornia-brachiata roxb r2r3-myb gene family dna-binding domain arabidopsis-thaliana transgenic arabidopsis dehydration tolerance ectopic expression abiotic stresses protein drought
Abstract Abiotic stresses like drought, salinity and extreme temperature significantly affect crop productivity. Plants respond at molecular, cellular and physiological levels for management of stress tolerance. Functional and regulatory genes play a major role in controlling these abiotic stresses through an intricate network of transcriptional machinery. Transcription factors are potential tools for manipulating stress tolerance since they control a large number of downstream genes. In the present study, we have isolated SbMYB44 from a succulent halophyte, Salicornia brachiata Roxb. SbMYB44 with an open-reading frame of 810 bp encodes a protein of 269 amino acids, with an estimated molecular mass of 30.31 kDa and an isoelectric point of 6.29. The in silico analysis revealed that the SbMYB44 protein contains the conserved R2R3 imperfect repeats, two SANT domains and post-translational modification sites. The SbMYB44 transcript showed up-regulation in response to salinity, desiccation, high temperature, and abscisic acid and salicylic acid treatments. The SbMYB44 recombinant protein showed binding to dehydration-responsive cis-elements (RD22 and MBS-1), suggesting its possible role in stress signalling. Overexpression of SbMYB44 enhanced the growth of yeast cells under both ionic and osmotic stresses.
Author Address [Shukla, Pushp Sheel; Agarwal, Parinita; Gupta, Kapil; Agarwal, Pradeep K.] CSIR, CSMCRI, Wasteland Res Div, Bhavnagar 364002, Gujarat, India. [Shukla, Pushp Sheel; Agarwal, Pradeep K.] CSIR, CSMCRI, Acad Sci & Innovat Res, Bhavnagar 364002, Gujarat, India. Agarwal, PK (reprint author), CSIR, CSMCRI, Wasteland Res Div, Gijubhai Badheka Marg, Bhavnagar 364002, Gujarat, India. pagarwal@csmcri.org
ISSN 2041-2851
ISBN 2041-2851
Year Published 2015
Volume 7
Digital Object Identifier (DOI) 10.1093/aobpla/plv054
Unique Article Identifier WOS:000358833700001
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