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Publication Type J
Authors Sanadhya, P., P. Agarwal, J. Khedia and P. K. Agarwal
Title A Low-Affinity K+ Transporter AlHKT2;1 from Recretohalophyte Aeluropus lagopoides Confers Salt Tolerance in Yeast
Source Molecular Biotechnology
Author Keywords Aeluropus lagopoides HKT transporter Salt stress AlHKT2 1 S. cerevisiae HKT TRANSPORTERS POTASSIUM TRANSPORTER CATION TRANSPORTER SACCHAROMYCES-CEREVISIAE SODIUM-TRANSPORT ARABIDOPSIS PLANTS RICE SELECTIVITY CLONING
Abstract The high-affinity potassium transporters (HKT) are highly important for stress tolerance in plants as they uniquely maintain K+/Na+ ratio for their survival and growth. In this study a novel HKT gene AlHKT2;1 was isolated and characterized from salt secreting halophyte, Aeluropus lagopoides. The AlHKT2;1 cDNA comprised of an open reading frame of 1,581 bp, encoding a protein of 526 amino acid residues. It belongs to class II HKTs and showed high homology with other HKT genes. Functional characterization of AlHKT2;1 in both K+ uptakeaEurodeficient (W Delta 6) and Na+-sensitive yeast mutants (G19) showed the characteristic feature of low-affinity K+ transporter supporting the growth at > 1 mM KCl concentration. The transformed yeast cells showed high sensitivity to NaCl; however, the addition of KCl along with NaCl support the growth of AlHKT2;1 expressing mutant. Ion content analysis of yeast cells with AlHKT2;1 grown in high NaCl medium supplemented with KCl revealed that salt tolerance was correlated with accumulation of K+ during salt stress. These results suggest that AlHKT2;1 plays an important role in the K+ uptake during salt stress and in maintaining a high K+/Na+ ratio in the cytosol.
Author Address [Sanadhya, Payal; Agarwal, Parinita; Khedia, Jackson; Agarwal, Pradeep K.] CSIR, CSIR CSMCRI, Discipline Wasteland Res, Bhavnagar 364002, Gujarat, India. [Sanadhya, Payal; Khedia, Jackson; Agarwal, Pradeep K.] CSIR, CSIR CSMCRI, Acad Sci & Innovat Res, Bhavnagar 364002, Gujarat, India. Agarwal, PK (reprint author), CSIR, CSIR CSMCRI, Discipline Wasteland Res, Gijubhai Badheka Marg, Bhavnagar 364002, Gujarat, India. pagarwal@csmcri.org
ISSN 1073-6085
ISBN 1073-6085
29-Character Source Abbreviation Mol. Biotechnol.
Publication Date Jun
Year Published 2015
Volume 57
Issue 6
Beginning Page 489-498
Digital Object Identifier (DOI) 10.1007/s12033-015-9842-9
Unique Article Identifier WOS:000354462100001
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