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Publication Type J
Authors Krishnamurthy, P., X. F. Tan, T. K. Lim, T. M. Lim, P. P. Kumar, C. S. Loh and Q. S. Lin
Title Proteomic analysis of plasma membrane and tonoplast from the leaves of mangrove plant Avicennia officinalis
Source Proteomics
Author Keywords Avicennia officinalis Plant proteomics Plasma membrane Salt secretion Tonoplast h+-translocating atpases salt-responsive proteins marina forsskal vierh heat-shock proteins arabidopsis-thaliana mesophyll-cells stress-response signal-transduction salinity tolerance expression
Abstract In order to understand the salt tolerance and secretion in mangrove plant species, gel electrophoresis coupled with LC-MS-based proteomics was used to identify key transport proteins in the plasma membrane (PM) and tonoplast fractions of Avicennia officinalis leaves. PM and tonoplast proteins were purified using two-aqueous-phase partitioning and density gradient centrifugation, respectively. Forty of the 254 PM proteins and 31 of the 165 tonoplast proteins identified were predicted to have transmembrane domains. About 95% of the identified proteins could be classified based on their functions. The major classes of proteins were predicted to be involved in transport, metabolic processes, defense/stress response, and signal transduction, while a few of the proteins were predicted to be involved in other functions such as membrane trafficking. The main classes of transporter proteins identified included H+-ATPases, ATP-binding cassette transporters, and aquaporins, all of which could play a role in salt secretion. These data will serve as the baseline membrane proteomic dataset for Avicennia species. Further, this information can contribute to future studies on understanding the mechanism of salt tolerance in halophytes in addition to salt secretion in mangroves. All MS data have been deposited in the ProteomeXchange with identifier PXD000837 (http://proteomecentral.proteomexchange.org/dataset/PXD000837).
Author Address [Krishnamurthy, Pannaga; Tan, Xing Fei; Lim, Teck Kwang; Lim, Tit-Meng; Kumar, Prakash P.; Loh, Chiang-Shiong; Lin, Qingsong] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore. [Krishnamurthy, Pannaga; Loh, Chiang-Shiong] Natl Univ Singapore, NUS Environm Res Inst NERI, Singapore 117548, Singapore. Lin, QS (reprint author), Natl Univ Singapore, Dept Biol Sci, 14 Sci Dr 4, Singapore 117543, Singapore. dbslinqs@nus.edu.sg
ISSN 1615-9853
ISBN 1615-9853
29-Character Source Abbreviation Proteomics
Publication Date Nov
Year Published 2014
Volume 14
Issue 21-22
Beginning Page 2545-2557
Digital Object Identifier (DOI) 10.1002/pmic.201300527
Unique Article Identifier WOS:000344807600019
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