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Publication Type J
Authors Ahmed, M. Z., T. Shimazaki, S. Gulzar, A. Kikuchi, B. Gul, M. A. Khan, H. W. Koyro, B. Huchzermeyer and K. N. Watanabe
Title The influence of genes regulating transmembrane transport of Na+ on the salt resistance of Aeluropus lagopoides
Source Functional Plant Biology
Author Keywords gene expression growth ion regulation Na+ sequestration photosynthesis salt stress plant-water status salinity tolerance antiporter gene mesembryanthemum-crystallinum plasma-membrane suaeda-salsa aestivum l. stress expression responses
Abstract Plantlets of Aeluropus lagopoides (Linn.) Trin. Ex Thw. were grown at different NaCl concentrations (26, 167, 373 and 747 mM) for 3, 7 and 15 days; their growth, osmotic adjustment, gas exchange, ion compartmentalisation and expression of various genes related to Na+ flux was studied. Plantlets showed optimal growth in non-saline (control; 26mM NaCl) solutions, whereas CO2/H2O gas exchange, leaf water concentration and water use efficiency decreased under all salinity treatments, accompanied by increased leaf senescence, root ash, sodium content and leaf osmolality. A decrease in malondialdehyde (MDA) content with time was correlated with Na+ accumulation in the leaf apoplast and a concomitant increase in Na+ secretion rate. A. lagopoides accumulated a higher concentration of Na+ in root than in leaf vacuoles, corresponding with higher expression of V-NHX and lower expression of PM-NHX in root than leaf tissue. It appears that V-ATPase plays a vital role during Na+ transport by producing an electromotive force, driving ion transport. Leaf calcium increased with increasing salinity, with more rapid accumulation at high salinity than at low salinity, indicating a possible involvement of Ca2+ in maintaining K+ : Na+ ratio. Our results suggest that A. lagopoides successfully compartmentalised Na+ at salinities up to 373mM NaCl by upregulating the gene expression of membrane linked transport proteins (V-NHX and PM-NHX). At higher salinity (747mMNaCl), a reduction in the expression of V-NHX and PM-NHX in leaves without any change in the rate of salt secretion, is a possible cause of the toxicity of NaCl.
Author Address [Ahmed, Muhammad Zaheer; Gulzar, Salman; Gul, Bilquees; Khan, M. Ajmal] Univ Karachi, Inst Sustainable Halophyte Utilisat, Karachi 75270, Pakistan. [Ahmed, Muhammad Zaheer; Shimazaki, Takayoshi; Kikuchi, Akira; Watanabe, Kazuo N.] Univ Tsukuba, Ctr Gene Res, Tsukuba, Ibaraki 3058572, Japan. [Khan, M. Ajmal] Qatar Univ, Coll Arts & Sci, Dept Int Affairs, Qatar Shell Professorial Chair Sustainable Dev, Doha, Qatar. [Koyro, Hans-W.] Univ Giessen, Inst Plant Ecol, D-35392 Giessen, Germany. [Huchzermeyer, Bernhard] Leibniz Univ Hannover, Inst Bot, D-30419 Hannover, Germany. Khan, MA (reprint author), Univ Karachi, Inst Sustainable Halophyte Utilisat, Karachi 75270, Pakistan. ajmal.khan@qu.edu.qa
ISSN 1445-4408
ISBN 1445-4408
29-Character Source Abbreviation Funct. Plant Biol.
Year Published 2013
Volume 40
Issue 8-9
Beginning Page 860-871
Digital Object Identifier (DOI) 10.1071/fp12346
Unique Article Identifier WOS:000322672000009
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