Loading content, please wait..
loading..
Logo
Version 3.20
or
Publication Type J
Authors Yi, X. P., Y. Sun, Q. Yang, A. P. Guo, L. Chang, D. Wang, Z. Tong, X. Jin, L. M. Wang, J. L. Yu, W. H. Jin, Y. M. Xie and X. C. Wang
Title Quantitative proteomics of Sesuvium portulacastrum leaves revealed that ion transportation by V-ATPase and sugar accumulation in chloroplast played crucial roles in halophyte salt tolerance
Source Journal of Proteomics
Author Keywords Halophyte Ion transportation Quantitative proteomics Sesuvium portulacastrum Sugar accumulation Vacuolar ATPase long-term salinity salicornia-europaea thellungiella-halophila proline accumulation arabidopsis-thaliana osmotic-stress comparative genomics responsive proteins gene-expression suaeda-salsa
Abstract Physiological and proteomic responses of Sesuvium portulacastrum leaves under salinity were investigated. Different from glycophytes, this halophyte had optimal growth at 200-300 mM NaCl and accumulated more starch grains in chloroplasts under high salinity. Increased contents of soluble sugars, proline, and Na+ were observed upon salinity. X-ray microanalysis revealed that Na+ was mainly compartmentalized into cell vacuole. Quantitative proteomics produced 96 salt responsive proteins, and the majority was chloroplast-located proteins. Gene ontology analysis revealed that proteins involved in ion binding, proton transport, photosynthesis and ATP synthesis were overrepresented. The expressions of a Na+/H+ antiporter and several ATP synthase subunits were activated upon high salinity. ATP hydrolysis assay demonstrated that V-ATPase activity at tonoplast was dramatically increased upon NaCl whereas vacuolar H+-pyrophosphatase and plasma membrane P-ATPase activities were not increased, which indicated that sodium compartmentalization was mainly performed by enhancing V-ATPase activity rather than P-ATPase and H+-pyrophosphatase. Accumulation of soluble sugars as well as sodium compartmentalization maintained the osmotic balance between vacuole and cytoplasm, which finally established ionic homeostasis in saline cells in true halophytes. Biological significance Physiological and proteomic analyses of S. portulacastrum leaves under different salinities were investigated. This true halophyte accumulated more soluble sugars, starch, proline and Na+ under high salinity. Differential proteomics produced 96 salt responsive proteins and the majority was involved in ion binding, proton transport, photosynthesis, and ATP synthesis. A Na+/H+ antiporter and several ATP synthase subunits were induced upon high salinity. ATP hydrolysis assay demonstrated that V-ATPase activity at tonoplast was dramatically increased whereas vacuolar H+-pyrophosphatase and plasma membrane ATPase activities were stable upon NaCl. These findings demonstrated that the increased Na+ was compartmentalized into vacuole by enhancing V-ATPase activity rather than H+-ATPase. Crown Copyright (C) 2014 Published by Elsevier B.V. All rights reserved.
ISSN 1874-3919
ISBN 1874-3919
29-Character Source Abbreviation J. Proteomics
Publication Date Mar
Year Published 2014
Volume 99
Beginning Page 84-100
Digital Object Identifier (DOI) 10.1016/j.jprot.2014.01.017
Unique Article Identifier WOS:000334010100006
Plants associated with this reference

LEGAL NOTICES — This website is protected by Copyright © The University of Sussex, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020. The eHALOPH database is protected by Database Right and Copyright © The University of Sussex and other contributors, 2006, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020. This database is based on an earlier work by James Aronson.
THIS WEBSITE AND THIS DATABASE ARE PROVIDED ON AN "AS IS" BASIS, AND YOU USE THEM AND RELY ON THEM AT YOUR OWN RISK.

Contact email: halophytes@sussex.ac.uk
Credits – Tim Flowers, Joaquim Santos, Moritz Jahns, Brian Warburton, Peter Reed