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Publication Type J
Authors Zhang, L., X. L. Ma, Q. Zhang, C. L. Ma, P. P. Wang, Y. F. Sun, Y. X. Zhao and H. Zhang
Title Expressed sequence tags from a NaCl-treated Suaeda salsa cDNA library
Source Gene
Language English
Author Keywords salt tolerance functional analyses expressed sequence tags halophytic model Suaeda salsa osmotic-stress saccharomyces-cerevisiae freezing tolerance protein-kinase ice plant arabidopsis yeast overexpression adaptations metabolism Genetics & Heredity
Abstract Past efforts to improve plant tolerance to osmotic stress have had limited success owing to the genetic complexity of stress responses. The first step towards cataloging and categorizing genetically complex abotic stress responses is the rapid discovery of genes by the large-scale partial sequencing of randomly selected cDNA clones or expressed sequence tags (ESTs). Suaeda salsa, which can survive seawater-level salinity, is a favorite halophytic model for salt tolerant research. We constructed a NaCl-treated cDNA library of Suaeda salsa and sequenced 1048 randomly selected clones, out of which 1016 clones produced readable sequences (773 showed homology to previously identified genes, 227 matched unknown protein coding regions, 16 anomalous sequences or sequences of bacterial origin were excluded from further analysis). By sequence analysis we identified 492 unique clones: 315 showed homology to previously identified genes, 177 matched unknown protein coding regions (101 of which have been found before in other organisms and 76 are completely novel). All our EST data are available on the Internet. We believe that our dbEST and the associated DNA materials will be a useful source to scientists engaging in stress-tolerance study. (C) 2001 Elsevier Science B.V. All rights reserved.
Author Address Shandong Normal Univ, Dept Biol, Key Lab Plant Stress Res, Jinan 250014, Shandong Provin, Peoples R China. Zhang, H (reprint author), Shandong Normal Univ, Dept Biol, Key Lab Plant Stress Res, 88 Wenhua E Rd, Jinan 250014, Shandong Provin, Peoples R China.
ISSN 0378-1119
ISBN 0378-1119
29-Character Source Abbreviation Gene
Publication Date Apr
Year Published 2001
Volume 267
Issue 2
Beginning Page 193-200
Digital Object Identifier (DOI) 10.1016/s0378-1119(01)00403-6
Unique Article Identifier WOS:000168416600006
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