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Publication Type J
Authors Yuan, F., M. Chen, B. Y. Leng and B. S. Wang
Title An efficient autofluorescence method for screening Limonium bicolor mutants for abnormal salt gland density and salt secretion
Source South African Journal of Botany
Author Keywords Autofluorescence Limonium bicolor Salt glands Mutant Salt tolerance fluorescence microscopy salinity tolerance drought tolerance induced mutations stomatal density gamma-radiation leaf-area arabidopsis protein plant
Abstract With the spread of saline soils worldwide, it has become increasingly important to understand salt-tolerant mechanisms and to develop halophytes with increased salt tolerance. Limonium bicolor is a typical recretohalophyte and has a typical salt excretory structure in the epidermis called the salt gland. A method that can be used to screen a large population of L bicolor mutants for altered salt gland density and altered salt secretion is needed but is currently unavailable. Leaves of 1-month-old L bicolor seedlings were processed by three traditional methods [epidermal peel, nail impression, and clearing/differential interference contrast microscope (clearing/DIC) method] and a fluorescence method (fluorescence microscopic examination of cleared leaves). With the fluorescence method, the autofluorescence of salt glands under UV excitation (330-380 nm) was easily distinguished with the least labor and time. The fluorescence method was used to screen similar to 10,000 seedlings (which grew from gamma-irradiated seeds). Four mutants with reduced salt gland density and 15 with increased salt gland density were obtained. Both kinds of mutants will be useful for the isolation of genes involved in salt gland development and salt secretion in L bicolor and other halophytes. The fluorescence method was also successfully used to observe the salt glands of Aegialitis rotundifolia and the stomata and trichomes of Arabidopsis. The fluorescence method described here will be useful for examining plant epidermal structures that have autofluorescence under UV or other wavelengths. (C) 2013 SAAB. Published by Elsevier B.V. All rights reserved.
Author Address [Yuan, F.; Chen, M.; Leng, B. Y.; Wang, B. S.] Shandong Normal Univ, Coll Life Sci, Key Lab Plant Stress Res, Jinan 250014, Shandong, Peoples R China. Wang, BS (reprint author), Shandong Normal Univ, Coll Life Sci, Key Lab Plant Stress Res, Jinan 250014, Shandong, Peoples R China. yuanfang19861114@126.com; chenminrundong@126.com; lengbingying@sina.cn; bswang@sdnu.edu.cn
ISSN 0254-6299
ISBN 0254-6299
29-Character Source Abbreviation S. Afr. J. Bot.
Publication Date Sep
Year Published 2013
Volume 88
Beginning Page 110-117
Digital Object Identifier (DOI) 10.1016/j.sajb.2013.06.007
Unique Article Identifier WOS:000328179500018
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