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Publication Type J
Authors Yu, J. J., S. X. Chen, Q. Zhao, T. Wang, C. P. Yang, C. Diaz, G. R. Sun and S. J. Dai
Title Physiological and Proteomic Analysis of Salinity Tolerance in Puccinellia tenuiflora
Source Journal of Proteome Research
Author Keywords proteomics halophyte Puccinellia tenuiflora salinity tolerance expressed sequence tags pentose-phosphate pathway improves salt tolerance chlorophyll fluorescence photosynthetic responses thellungiella-halophila transgenic tobacco molecular-cloning alpha-tocopherol gene-expression
Abstract Soil salinity poses a serious threat to agriculture productivity throughout the world. Studying mechanisms of salinity tolerance in halophytic plants will provide valuable information for engineering plants for enhanced salt tolerance. Monocotyledonous Puecinellia tenuiflora is a halophytic species that widely distributed in the saline-alkali soil of the Songnen plain in northeastern China. Here we investigate the molecular mechanisms underlying moderate salt tolerance of P. tenuiflora using a combined physiological and proteomic approach. The changes in biomass, inorganic ion content, osmolytes, photosynthesis, defense-related enzyme activities, and metabolites in the course of salt treatment were analyzed in the leaves. Comparative proteomic analysis revealed 107 identities (representing 93 unique proteins) differentially expressed in P. tenuiflora leaves under saline conditions. These proteins were mainly involved in photosynthesis, stress and defense, carbohydrate and energy metabolism, protein metabolism, signaling, membrane, and transport. Our results showed that reduction of photosynthesis under salt treatment was attributed to the down-regulation of the light-harvesting complex (LHC) and Calvin cycle enzymes. Selective uptake of inorganic ions, high K(+)/Na(+) ratio, Ca(2+) concentration changes, and an accumulation of osmolytes contributed to ion balance and osmotic adjustment in leaf cells. Importantly, P. tenuiflora plants developed diverse reactive oxygen species (ROS) scavenging mechanisms in their leaves to cope with moderate salinity, including enhancement of the photorespiration pathway and thermal dissipation, synthesis of the low-molecular-weight antioxidant alpha-tocopherol, and an accumulation of compatible solutes. This study provides important information toward improving salt tolerance of cereals.
Author Address [Sun, GR] Binzhou Polytech Coll, Binzhou 256603, Shandong, Peoples R China [Yu, JJ; Zhao, Q; Yang, CP; Dai, SJ] NE Forestry Univ, Alkali Soil Nat Environm Sci Ctr, Key Lab Saline Alkali Vegetat Ecol Restorat Oil F, Minist Educ, Harbin 150040, Peoples R China [Chen, SX; Diaz, C] Univ Florida, Dept Biol, Genet Inst, Interdisciplinary Ctr Biotechnol Res, Gainesville, FL 32610 USA [Dai, SJ] Harbin Normal Univ, Coll Life Sci & Technol, Harbin 150080, Peoples R China [Wang, T] Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China Sun, GR (reprint author), Binzhou Polytech Coll, Binzhou 256603, Shandong, Peoples R China grsun@live.cn daishaojun@hotmail.com
ISSN 1535-3893
ISBN 1535-3893
29-Character Source Abbreviation J. Proteome Res.
Publication Date Sep
Year Published 2011
Volume 10
Issue 9
Beginning Page 3852-3870
Digital Object Identifier (DOI) 10.1021/pr101102p
Unique Article Identifier WOS:000294446600002
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