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Publication Type J
Authors You, Y. H., H. Yoon, S. M. Kang, J. H. Shin, Y. S. Choo, I. J. Lee, J. M. Lee and J. G. Kim
Title Fungal Diversity and Plant Growth Promotion of Endophytic Fungi from Six Halophytes in Suncheon Bay
Source Journal of Microbiology and Biotechnology
Author Keywords Endophytic fungi fungal diversity plant growth promotion gibberellin halophyte salt marsh carex-kobomugi ohwi gibberellin production metabolism stress strain
Abstract Endophytic fungi were isolated from roots of six halophytes in Suncheon Bay. The endophytic fungi of 35 species isolated from halophytes were identified by internal transcribed spacer (ITS) containing the ITSI, 5.8s, and ITS2 regions. All fungal strains were analyzed to diversity at the genus level. Fungal culture filtrates (FCF) of endophytic fungi were treated to Waito-c rice (WR) seedling for plant growth-promoting verification. It was confirmed that fungal strain Sj-2-2 provided plant growth promotion (PGP) to WR seedling. Then, PGP of Suaeda japonica was confirmed by treating culture filtrate of Sj-2-2. As a result, it was verified that culture filtrate of Sj-2-2 had more advanced PGP than positive control when treated to S. japonica. The secondary metabolites involved in culture filtrate of Sj-2-2 were identified by HPLC and GC-MS SIM analysis. The presence of physiologically bioactive gibberellins (GAs) and other inactive GAs in culture filtrate of Sj-2-2 was detected. The molecular analysis of sequences of Sj-2-2 showed the similarity to Penicillium sp. of 99% homology. The PGP of Sj-2-2 as well as symbiosis between endophytic fungi and halophytes growing naturally in salt marsh was confirmed. Sj-2-2 was identified as a new fungal strain producing GAs by molecular analysis of sequences. Consequently, the Sj-2-2 fungal strain was named as Penicillium sp. Sj-2-2. In this study, the diversity of endophytic fungi isolated from roots of halophytes in salt marsh and the PGP of a new gibberellin-producing fungal strain were confirmed.
Author Address [You, Young-Hyun; Yoon, Hyeokjun; Kim, Jong-Guk] Kyungpook Natl Univ, Dept Life Sci & Biotechnol, Taegu 702701, South Korea. [Kang, Sang-Mo; Shin, Jae-Ho; Lee, In-Jung] Kyungpook Natl Univ, Sch Appl Biosci, Taegu 702701, South Korea. [Choo, Yeon-Sik] Kyungpook Natl Univ, Dept Biol, Coll Natl Sci, Taegu 702701, South Korea. [Lee, Jin-Man] Hoseo Univ, Dept Food Sci & Technol, Asan 336795, South Korea. [Lee, Jin-Man] Hoseo Univ, Inst Basic Sci, Asan 336795, South Korea. Kim, JG (reprint author), Kyungpook Natl Univ, Dept Life Sci & Biotechnol, Taegu 702701, South Korea. kimjg@knu.ac.kr
ISSN 1017-7825
ISBN 1017-7825
29-Character Source Abbreviation J. Microbiol. Biotechnol.
Publication Date Nov
Year Published 2012
Volume 22
Issue 11
Beginning Page 1549-1556
Digital Object Identifier (DOI) 10.4014/jmb.1205.05010
Unique Article Identifier WOS:000311761600014
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