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Publication Type J
Authors Yang, Y. L., Y. Y. Zhang, J. Lu, H. Zhang, Y. Liu, Y. Jiang and R. X. Shi
Title Exogenous H2O2 increased catalase and peroxidase activities and proline content in Nitraria tangutorum callus
Source Biologia Plantarum
Author Keywords antioxidative enzymes calcium gamma-glutamyl kinase proline dehydrogenase hydrogen-peroxide arabidopsis-thaliana oxidative stress salt stress signal-transduction tolerance accumulation cells expression nacl
Abstract Antioxidative responses and proline accumulation induced by exogenous H2O2 were investigated in the callus from halophyte Nitraria tangutorum Bobr. H2O2-treated callus exhibited higher H2O2 content than untreated callus. The activities of catalase (CAT) and peroxidase (POD) significantly increased in the callus treated with H2O2, while ascorbate peroxidase (APX) activity decreased. In addition, significantly enhanced proline content was observed in the callus treated by H2O2, which could be alleviated by H2O2 scavenger dimethylthiourea and calcium (Ca) chelator ethylene glycol bis-(beta-aminoethyl ether)-N,N,N',N'-tetra-acetic acid (EGTA). Moreover, gamma-glutamyl kinase (GK) activity increased in H2O2-treated callus, but proline dehydrogenase (PDH) activity decreased significantly, and the reduction was partly abolished by EGTA or Ca channel blocker verapamil. Assays using a scanning electron microscope showed significantly enhanced Ca content in H2O2-treated callus.
Author Address [Yang, Y. L.; Zhang, Y. Y.; Lu, J.; Zhang, H.; Liu, Y.; Jiang, Y.; Shi, R. X.] NW Normal Univ, Sch Life Sci, Lanzhou 730070, Peoples R China. Yang, YL (reprint author), NW Normal Univ, Sch Life Sci, Lanzhou 730070, Peoples R China xbsfyangyingli@163.com
ISSN 0006-3134
ISBN 0006-3134
29-Character Source Abbreviation Biol. Plant.
Publication Date Jun
Year Published 2012
Volume 56
Issue 2
Beginning Page 330-336
Digital Object Identifier (DOI) 10.1007/s10535-012-0094-2
Unique Article Identifier WOS:000301179100018
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