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Version 3.21
Publication Type J
Authors Xing, K., S. Qin, G. K. Bian, Y. J. Zhang, W. D. Zhang, C. C. Dai, C. H. Liu, W. J. Li and J. H. Jiang
Title Pseudonocardia nantongensis sp nov., a novel endophytic actinomycete isolated from the coastal halophyte Tamarix chinensis Lour
Source Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
Author Keywords Pseudonocardia nantongensis sp nov Coastal halophyte Endophytic Polyphasic taxonomy emended genus pseudonocardia deoxyribonucleic-acid maximum-likelihood proposal sequences nocardia combine china trees root
Abstract A novel isolate, designated strain KLBMP 1282(T) was isolated from the surface-sterilized leaves of a coastal halophyte Tamarix chinensis Lour., collected from Nantong, Jiangsu Province, east of China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that this strain belongs to the genus Pseudonocardia, being most closely related to Pseudonocardia kongjuensis LM 157(T) (98.33 %), Pseudonocardia autotrophica IMSNU 20050(T) (97.77 %), Pseudonocardia endophytica YIM 56035(T) (97.63 %), Pseudonocardia ammonioxydans H9 (T) (97.62 %) and Pseudonocardia compacta IMSNU 20111(T) (97.56 %); similarity to other type strains of the genus Pseudonocardia was < 97.5 %. Chemotaxonomic data confirmed the affiliation of strain KLBMP 1282(T) to the genus Pseudonocardia. Strain KLBMP 1282(T) contained MK-8(H-4) as the predominant ubiquinone and iso-C-16:0 as the major fatty acid. The polar lipids detected in strain KLBMP 1282(T) were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, one unknown phospholipid and four unknown glycolipids. The DNA G + C content of strain KLBMP 1282(T) was 73.1 mol %. The results of DNA-DNA hybridizations and the phylogenetic analysis, together with the phenotypic and biochemical tests, allowed the differentiation of strain KLBMP 1282(T) from strains of other recognized Pseudonocardia species. Therefore, strain KLBMP 1282(T) represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia nantongensis sp. nov. is proposed. The type strain is KLBMP 1282(T) (=KCTC 29053(T) = NBRC 108677(T)).
Author Address [Xing, Ke; Qin, Sheng; Bian, Guang-Kai; Zhang, Yue-Ji; Zhang, Wen-Di; Jiang, Ji-Hong] Jiangsu Normal Univ, Key Lab Biotechnol Med Plant Jiangsu Prov, Sch Life Sci, Xuzhou 221116, Jiangsu, Peoples R China. [Dai, Chuan-Chao] Nanjing Normal Univ, Coll Life Sci, Nanjing 210046, Jiangsu, Peoples R China. [Liu, Chang-Hong] Nanjing Univ, Coll Life Sci, Nanjing 210093, Jiangsu, Peoples R China. [Li, Wen-Jun] Yunnan Univ, Key Lab Microbial Resources, Minist Educ, Yunnan 650091, Peoples R China. [Li, Wen-Jun] Yunnan Univ, Lab Conservat & Utilizat Bioresources, Yunnan Inst Microbiol, Yunnan 650091, Peoples R China. Qin, S (reprint author), Jiangsu Normal Univ, Key Lab Biotechnol Med Plant Jiangsu Prov, Sch Life Sci, Xuzhou 221116, Jiangsu, Peoples R China. shengqin@jsnu.edu.cn; jhjiang@jsnu.edu.cn
ISSN 0003-6072
ISBN 0003-6072
29-Character Source Abbreviation Antonie Van Leeuwenhoek
Publication Date Nov
Year Published 2012
Volume 102
Issue 4
Beginning Page 659-667
Digital Object Identifier (DOI) 10.1007/s10482-012-9764-5
Unique Article Identifier WOS:000310026300014
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