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Publication Type J
Authors Seal, C. E., R. Zammit, P. Scott, T. J. Flowers and I. Kranner
Title Glutathione half-cell reduction potential and alpha-tocopherol as viability markers during the prolonged storage of Suaeda maritima seeds
Source Seed Science Research
Author Keywords ageing electrolyte leakage glutathione seed Suaeda maritima tocopherol viability lipid-peroxidation oxidative stress membrane damage salt tolerance desiccation tolerance biochemical-changes active oxygen l. seeds l dum recalcitrant
Abstract Antioxidants protect seeds from oxidative damage during storage, supporting the maintenance of seed viability and the ability to germinate post-storage. No data on antioxidants during long-term storage are available for the seeds of the halophyte Suaeda maritima. Therefore, changes in lipid-soluble antioxidants in the tocopherol family (alpha-, gamma-, delta-tocopherol), were investigated in seeds stored for up to 16 years at 40 degrees C at a seed moisture content of 10-13%, as we as changes in the water-soluble antioxidant glutathione (GSH) and electrolyte leakage. Seed oil content was also measured and determined to be 22%. During the first 3 years of storage, seed viability remained high and the concentration of total tocopherol was stable. Thereafter both seed viability and alpha-tocopherol concentration rapidly decreased and electrolyte leakage increased while gamma-tocopherol and delta-tocopherol concentrations did not correlate with seed viability. Although the concentrations of neither GSH nor glutathione disulphide (GSSG) alone were correlated with seed viability, the glutathione half-cell reduction potential (E(GSSG/2GSH)) was strongly correlated with viability throughout storage and increased before the onset of viability loss. Hence in this species E(GSSG/2GSH) appeared to be an 'early warning' system preceding viability loss while alpha-tocopherol concentration changed concomitantly with viability.
Author Address [Seal, CE; Kranner, I] Royal Bot Gardens, Seed Conservat Dept, Richmond RH17 6TN, W Sussex, England [Zammit, R; Scott, P; Flowers, TJ] Univ Sussex, Sch Life Sci, Brighton BN1 9QG, E Sussex, England Seal, CE (reprint author), Royal Bot Gardens, Seed Conservat Dept, Wakehurst Pl, Richmond RH17 6TN, W Sussex, England c.seal@kew.org
ISSN 0960-2585
ISBN 0960-2585
29-Character Source Abbreviation Seed Sci. Res.
Publication Date Mar
Year Published 2010
Volume 20
Issue 1
Beginning Page 47-53
Digital Object Identifier (DOI) 10.1017/s0960258509990250
Unique Article Identifier WOS:000274691600006
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