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Publication Type J
Authors Sonjak, S., T. Beguiristain, C. Leyval and M. Regvar
Title Temporal temperature gradient gel electrophoresis (TTGE) analysis of arbuscular mycorrhizal fungi associated with selected plants from saline and metal polluted environments
Source Plant and Soil
Author Keywords Arbuscular mycorrhizal fungi (AMF) Halophytes Metal pollution Salterns Thlaspi praecox TTGE HYPERACCUMULATOR THLASPI-PRAECOX HEAVY-METAL COLONIZATION DIVERSITY ROOTS EVOLUTION BRASSICACEAE COMMUNITIES ALIGNMENT SOILS
Abstract Some plants are more mycorrhizal than others and mycorrhizal colonisation of plants in extreme environments is frequently additionally reduced due to decreased spore density and/or diversity and therefore frequently overlooked. We analysed two plant species from both metal polluted and saline enriched soils with differing mycorrhizal colonisation levels/status using classical and molecular methods. The selected plant species were Sesleria caerulea (L.) Ard. and Thlaspi praecox Wulfen from a metal polluted site, and Limonium angustifolium (Tausch) Degen [Statice serotina Rchb., L. vulgare Mill. subsp. Serotinum (Rchb.) Gams] and Salicornia europaea L. from the Secovlje salterns in Slovenia. Despite the high mycorrhizal frequencies (F%) observed, the presence of arbuscules (A%) was at best low in S. caerulea and T. praecox, and undetectable in L. angustifolium and S. europaea. Temporal temperature gradient gel electrophoresis (TTGE) was applied to field-collected samples from both burdened environments and proved to be an effective technique for rapid profiling and identification of arbuscular mycorrhizal fungi (AMF). Sequencing and phylogenetic analysis confirmed the association of AMF of the genus Glomus with roots of all four plant species. This is the first report on the identification and profiling of Glomeromycota in the field-collected Cd/Zn metal hyperaccumulator T. praecox growing at a highly metal polluted site, as well as in L. angustifolium and S. europaea collected in a saline environment. The identification of AMF from both ecosystems only partially resembles previous identifications on the basis of spores.
Author Address [Sonjak, Silva; Regvar, Marjana] Univ Ljubljana, Biotech Fac, Dept Biol, SI-1000 Ljubljana, Slovenia. [Beguiristain, Thierry; Leyval, Corinne] Nancy Univ, CNRS, Lab Interact Microorganismes Mineraux Mat Organ S, UMR 7131,Fac Sci, F-54506 Vandoeuvre Les Nancy, France. Regvar, M, Univ Ljubljana, Biotech Fac, Dept Biol, Vecna Pot 111, SI-1000 Ljubljana, Slovenia. marjana.regvar@bf.uni-lj.si
ISSN 0032-079X
ISBN 0032-079X
29-Character Source Abbreviation Plant Soil
Publication Date Jan
Year Published 2009
Volume 314
Issue 1-2
Beginning Page 25-34
Digital Object Identifier (DOI) 10.1007/s11104-008-9702-5
Unique Article Identifier ISI:000261579500003
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