Loading content, please wait..
loading..
Logo
Version 3.20
or
Publication Type J
Authors Sobhanian, H., N. Motamed, F. R. Jazii, T. Nakamura and S. Komatsu
Title Salt Stress Induced Differential Proteome and Metabolome Response in the Shoots of Aeluropus lagopoides (Poaceae), a Halophyte C(4) Plant
Source Journal of Proteome Research
Author Keywords Aeluropus lagopoides C(4) photosynthesis halophyte metabolome proteome salt stress glyoxalase-i rice leaf brassica-juncea salinity stress gene family tolerance glutathione l. proteins growth
Abstract A proteomic approach was used to identify proteins affected by salt in the halophyte C(4) plant Aeluropus lagopoides (Poaceae) in an attempt to understand the mechanism of salt tolerance. Plants were treated with 450 mM NaCl for 10 days, and proteins were then extracted from the shoots and separated by two-dimensional polyacrylamide gel electrophoresis. A total of 1805 protein spots were detected, of which 39 were up-regulated and 44 were down-regulated by treatment with NaCl. Metabolism-related proteins were up-regulated, whereas photosynthesis-related proteins were down-regulated. Dose-dependence studies showed that the up-regulation continued at NaCl concentrations above 450 mM for defense-related proteins alone. Western blot analysis confirmed the down-regulation of RuBisCO LSU and RuBisCO SSU and severe down-regulation of RuBisCO activase. The activity of glyoxalase I increased with increasing NaCl concentration. Metabolome studies indicated up-regulation of amino acids and down-regulation of tricarboxylic acid cycle-related metabolites. These studies suggest that up-regulation of energy formation, amino acid biosynthesis, C(4) photosynthesis, and detoxification are the main strategies for salt tolerance in A. lagopoides.
Author Address [Sobhanian, H; Nakamura, T; Komatsu, S] Natl Inst Crop Sci, Tsukuba, Ibaraki 3058518, Japan [Sobhanian, H; Motamed, N] Univ Tehran, Coll Sci, Sch Biol, Tehran 141556455, Iran [Sobhanian, H; Jazii, FR] Natl Inst Genet Engn & Biotechnol, Tehran 141556343, Iran Komatsu, S (reprint author), Natl Inst Crop Sci, Tsukuba, Ibaraki 3058518, Japan skomatsu@affrc.go.jp
ISSN 1535-3893
ISBN 1535-3893
29-Character Source Abbreviation J. Proteome Res.
Publication Date Jun
Year Published 2010
Volume 9
Issue 6
Beginning Page 2882-2897
Digital Object Identifier (DOI) 10.1021/pr900974k
Unique Article Identifier WOS:000278243300009
Plants associated with this reference

LEGAL NOTICES — This website is protected by Copyright © The University of Sussex, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020. The eHALOPH database is protected by Database Right and Copyright © The University of Sussex and other contributors, 2006, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020. This database is based on an earlier work by James Aronson.
THIS WEBSITE AND THIS DATABASE ARE PROVIDED ON AN "AS IS" BASIS, AND YOU USE THEM AND RELY ON THEM AT YOUR OWN RISK.

Contact email: halophytes@sussex.ac.uk
Credits – Tim Flowers, Joaquim Santos, Moritz Jahns, Brian Warburton, Peter Reed